Initiating Antiretroviral Treatment Early in Infancy Has Long-term Benefits on the Human Immunodeficiency Virus Reservoir in Late Childhood and Adolescence.


Journal

Clinical infectious diseases : an official publication of the Infectious Diseases Society of America
ISSN: 1537-6591
Titre abrégé: Clin Infect Dis
Pays: United States
ID NLM: 9203213

Informations de publication

Date de publication:
06 12 2021
Historique:
received: 26 10 2020
pubmed: 7 8 2021
medline: 15 3 2022
entrez: 6 8 2021
Statut: ppublish

Résumé

Early combined antiretroviral therapy (cART) limits the total HIV-DNA load in children. However, data on its impact in older children and adolescents remain scarce. This study compares HIV reservoirs in children (5-12 years) and adolescents (13-17 years) who started cART <6 months (early [E-] group) or >2 years (late [L-] group). The ANRS-EP59-CLEAC study prospectively enrolled 76 patients perinatally infected with HIV-1 who reached HIV-RNA <400 copies/mL <24 months after cART initiation, regardless of subsequent viral suppression (E-group: 27 children, 9 adolescents; L-group: 19 children, 21 adolescents). Total and integrated HIV-DNA were quantified in blood and in CD4+ T-cell subsets. A substudy assessed HIV reservoir inducibility after ex vivo peripheral blood mononuclear cell (PBMC) stimulation. Total HIV-DNA levels were lower in early- versus late-treated patients (children: 2.14 vs 2.87 log copies/million PBMCs; adolescents: 2.25 vs 2.74 log; P < .0001 for both). Low reservoir was independently associated with treatment precocity, protective HLA, and low cumulative viremia since cART initiation. The 60 participants with undetectable integrated HIV-DNA started cART earlier than other patients (4 vs 54 months; P = .03). In those with sustained virological control, transitional and effector memory CD4+ T cells were less infected in the E-group than in the L-group (P = .03 and .02, respectively). Viral inducibility of reservoir cells after normalization to HIV-DNA levels was similar between groups. Early cART results in a smaller blood HIV reservoir until adolescence, but all tested participants had an inducible reservoir. This deserves cautious consideration for HIV remission strategies.

Sections du résumé

BACKGROUND
Early combined antiretroviral therapy (cART) limits the total HIV-DNA load in children. However, data on its impact in older children and adolescents remain scarce. This study compares HIV reservoirs in children (5-12 years) and adolescents (13-17 years) who started cART <6 months (early [E-] group) or >2 years (late [L-] group).
METHODS
The ANRS-EP59-CLEAC study prospectively enrolled 76 patients perinatally infected with HIV-1 who reached HIV-RNA <400 copies/mL <24 months after cART initiation, regardless of subsequent viral suppression (E-group: 27 children, 9 adolescents; L-group: 19 children, 21 adolescents). Total and integrated HIV-DNA were quantified in blood and in CD4+ T-cell subsets. A substudy assessed HIV reservoir inducibility after ex vivo peripheral blood mononuclear cell (PBMC) stimulation.
RESULTS
Total HIV-DNA levels were lower in early- versus late-treated patients (children: 2.14 vs 2.87 log copies/million PBMCs; adolescents: 2.25 vs 2.74 log; P < .0001 for both). Low reservoir was independently associated with treatment precocity, protective HLA, and low cumulative viremia since cART initiation. The 60 participants with undetectable integrated HIV-DNA started cART earlier than other patients (4 vs 54 months; P = .03). In those with sustained virological control, transitional and effector memory CD4+ T cells were less infected in the E-group than in the L-group (P = .03 and .02, respectively). Viral inducibility of reservoir cells after normalization to HIV-DNA levels was similar between groups.
CONCLUSIONS
Early cART results in a smaller blood HIV reservoir until adolescence, but all tested participants had an inducible reservoir. This deserves cautious consideration for HIV remission strategies.

Identifiants

pubmed: 34355738
pii: 6062433
doi: 10.1093/cid/ciaa1931
doi:

Substances chimiques

DNA, Viral 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

e4214-e4222

Subventions

Organisme : ANRS

Investigateurs

Mary-France Courcoux (MF)
Catherine Dollfus (C)
Marie-Dominique Tabone (MD)
Geneviève Vaudre (G)
Corinne Fourcade (C)
Josiane Warsazawski (J)
Jérôme Lechenadec (J)
Olivia Dialla (O)
Laura Nailler (L)
Lamya Ait Si Selmi (LAS)
Isabelle Leymarie (I)
Thierry Wack (T)
Alexandre Hoctin (A)
Razika Feraon-Nanache (R)
Isabelle Hau (I)
Cécile Gakobwa (C)
Véronique Avettand-Fenoël (V)
Stéphane Blanche (S)
Marine Fillion (M)
Pierre Frange (P)
Nizar Mahlaoui (N)
Adeline Mélard (A)
Florence Veber (F)
Marie-Christine Mourey (MC)
Valérie Marcou (V)
Albert Faye (A)
Martine Lévine (M)
Sandrine Richard (S)
Brigitte Autran (B)
Assia Samri (A)
Mariama Diallo (M)
Sophie Caillat-Zucman (S)
Kahina Amokrane (K)
Rayna Ivanova-Derin (R)
Anne Chacé (A)
Florence Buseyne (F)
Thomas Montange (T)
Damien Batalie (D)
Ingrid Fert (I)
Asier Saez-Cirion (A)
Valérie Monceaux (V)
Daniel Scott-Algara (D)
Lucie Marchand (L)
Delphine Lebrasseur (D)
Axel Levier (A)

Informations de copyright

© The Author(s) 2021. Published by Oxford University Press for the Infectious Diseases Society of America. All rights reserved. For permissions, e-mail: journals.permissions@oup.com.

Auteurs

Véronique Avettand-Fenoel (V)

Assistance Publique-Hôpitaux de Paris (AP-HP), Laboratoire de Microbiologie Clinique, Hôpital Necker-Enfants Malades, Paris, France.
Université de Paris, Faculté de Médecine, Paris, France.
Institut National de la Santé et de la Recherche Médicale (INSERM) U1016, Centre National de recherche Scientifique (CNRS) 8104, Institut Cochin, Paris,France.

Jérôme Lechenadec (J)

Département d'épidémiologie, Centre de Recherche en Epidémiologie et Santé des Populations, INSERM, Le Kremlin-Bicêtre, Villejuif, France.

Mariama Sadjo Diallo (MS)

Sorbonne Université, INSERM 1135, Centre d'immunologie et des Maladies Infectieuses, Cimi-Paris Paris, France.

Marine Fillion (M)

Université de Paris, Faculté de Médecine, Paris, France.
Institut National de la Santé et de la Recherche Médicale (INSERM) U1016, Centre National de recherche Scientifique (CNRS) 8104, Institut Cochin, Paris,France.

Adeline Melard (A)

Université de Paris, Faculté de Médecine, Paris, France.
Institut National de la Santé et de la Recherche Médicale (INSERM) U1016, Centre National de recherche Scientifique (CNRS) 8104, Institut Cochin, Paris,France.

Assia Samri (A)

Sorbonne Université, INSERM 1135, Centre d'immunologie et des Maladies Infectieuses, Cimi-Paris Paris, France.

Catherine Dollfus (C)

AP-HP Sorbonne Université, Service d'Hématologie-Oncologie Pédiatrique, Hôpital d'Enfants Armand Trousseau, Paris, France.

Stéphane Blanche (S)

Université de Paris, Faculté de Médecine, Paris, France.
AP-HP, Service d'Immuno-Hématologie Pédiatrique, Hôpital Necker-Enfants Malades, Paris, France.

Albert Faye (A)

Université de Paris, Faculté de Médecine, Paris, France.
AP-HP, Pédiatrie Générale et Maladies Infectieuses, Hôpital Robert Debré, Paris, France.

Kahina Amokrane (K)

Université de Paris, Faculté de Médecine, Paris, France.
AP-HP, Laboratoire d'Immunologie et Histocompatibilité, Hôpital St-Louis, Paris, France.

Brigitte Autran (B)

Sorbonne Université, INSERM 1135, Centre d'immunologie et des Maladies Infectieuses, Cimi-Paris Paris, France.

Florence Buseyne (F)

Unité d'Épidémiologie et Physiopathologie des Virus Oncogènes, Institut Pasteur, Paris, France.
CNRS 3569, Paris, France.

Josiane Warszawski (J)

Département d'épidémiologie, Centre de Recherche en Epidémiologie et Santé des Populations, INSERM, Le Kremlin-Bicêtre, Villejuif, France.

Pierre Frange (P)

Assistance Publique-Hôpitaux de Paris (AP-HP), Laboratoire de Microbiologie Clinique, Hôpital Necker-Enfants Malades, Paris, France.
Université de Paris, Faculté de Médecine, Paris, France.
Equipe Hopistalo-Universitaire 7328, Institut Imagine, Université de Paris, Paris, France.

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