Maintenance of Ligament Homeostasis of Spheroid-Colonized Embroidered and Functionalized Scaffolds after 3D Stretch.
Animals
Anterior Cruciate Ligament
/ cytology
Cell Adhesion
Cell Proliferation
Cells, Cultured
Connexins
/ genetics
Decorin
/ genetics
Extracellular Matrix Proteins
/ genetics
Female
Fibroblasts
/ physiology
Homeodomain Proteins
/ genetics
Homeostasis
Male
Polyesters
/ chemistry
Rabbits
Regeneration
Spheroids, Cellular
/ cytology
Stress, Mechanical
Tissue Engineering
/ methods
Tissue Scaffolds
/ chemistry
3D stretch
ACL
embroidered functionalized scaffold
fluorine
ligament tissue engineering
poly(l-lactide-co-ε-caprolactone (P(LA-CL))
polylactic acid (PLA)
spheroids
Journal
International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791
Informations de publication
Date de publication:
30 Jul 2021
30 Jul 2021
Historique:
received:
18
06
2021
revised:
18
07
2021
accepted:
23
07
2021
entrez:
7
8
2021
pubmed:
8
8
2021
medline:
9
9
2021
Statut:
epublish
Résumé
Anterior cruciate ligament (ACL) ruptures are usually treated with autograft implantation to prevent knee instability. Tissue engineered ACL reconstruction is becoming promising to circumvent autograft limitations. The aim was to evaluate the influence of cyclic stretch on lapine (L) ACL fibroblasts on embroidered scaffolds with respect to adhesion, DNA and sulphated glycosaminoglycan (sGAG) contents, gene expression of ligament-associated extracellular matrix genes, such as type I collagen, decorin, tenascin C, tenomodulin, gap junctional connexin 43 and the transcription factor Mohawk. Control scaffolds and those functionalized by gas phase fluorination and cross-linked collagen foam were either pre-cultured with a suspension or with spheroids of LACL cells before being subjected to cyclic stretch (4%, 0.11 Hz, 3 days). Stretch increased significantly the scaffold area colonized with cells but impaired sGAGs and decorin gene expression (functionalized scaffolds seeded with cell suspension). Stretching increased tenascin C, connexin 43 and Mohawk but decreased decorin gene expression (control scaffolds seeded with cell suspension). Pre-cultivation of functionalized scaffolds with spheroids might be the more suitable method for maintaining ligamentogenesis in 3D scaffolds compared to using a cell suspension due to a significantly higher sGAG content in response to stretching and type I collagen gene expression in functionalized scaffolds.
Identifiants
pubmed: 34360970
pii: ijms22158204
doi: 10.3390/ijms22158204
pmc: PMC8348491
pii:
doi:
Substances chimiques
Connexins
0
Decorin
0
Extracellular Matrix Proteins
0
Homeodomain Proteins
0
Polyesters
0
lactide-caprolactone copolymer
70524-20-8
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Deutsche Forschungsgemeinschaft
ID : ME 3734/2-1, ME 3734/5-1, SCHU 1979/9-1, SCHU 1979/14-1, BR 1886/6-1, BR 5604/1-1, HE 4466/22-1,
Références
Int J Mol Sci. 2019 Apr 22;20(8):
pubmed: 31013670
J Biol Chem. 2008 Oct 3;283(40):26839-49
pubmed: 18669633
Biochem Biophys Res Commun. 2019 May 14;512(4):691-697
pubmed: 30922565
Am J Physiol Regul Integr Comp Physiol. 2007 Nov;293(5):R1898-907
pubmed: 17686880
Biomech Model Mechanobiol. 2015 Jun;14(3):649-63
pubmed: 25351891
J Biomed Mater Res A. 2006 Jun 1;77(3):639-52
pubmed: 16550538
Tissue Eng Regen Med. 2019 Jun 26;16(6):549-571
pubmed: 31824819
J Biomech. 1998 Jun;31(6):519-25
pubmed: 9755036
J Mater Sci Mater Med. 2012 Oct;23(10):2575-82
pubmed: 22729594
J Biomed Mater Res A. 2014 May;102(5):1614-24
pubmed: 23737190
Mol Cell Biol. 2016 Mar 31;36(8):1297-309
pubmed: 26884464
Tissue Eng. 2006 Jul;12(7):1843-9
pubmed: 16889514
J Orthop Res. 2015 Jun;33(6):911-8
pubmed: 25664422
Sci Adv. 2018 Jan 17;4(1):eaao4881
pubmed: 29349300
Stem Cell Rev Rep. 2016 Apr;12(2):189-201
pubmed: 26661573
Birth Defects Res C Embryo Today. 2013 Sep;99(3):203-222
pubmed: 24078497
Matrix Biol. 2017 Dec;64:81-93
pubmed: 28882761
Arthroscopy. 2001 Nov-Dec;17(9):971-80
pubmed: 11694930
Cell Physiol Biochem. 2017;41(3):875-889
pubmed: 28214894
Mol Biol Cell. 2001 Sep;12(9):2730-41
pubmed: 11553712
Biomaterials. 2010 Dec;31(36):9438-51
pubmed: 20870282
ACS Omega. 2020 Mar 05;5(10):5498-5507
pubmed: 32201842
Mater Sci Eng C Mater Biol Appl. 2014 Oct;43:290-9
pubmed: 25175216
Clin Orthop Relat Res. 1995 Sep;(318):265-78
pubmed: 7671527
Matrix Biol. 2010 Oct;29(8):678-89
pubmed: 20736063
Stem Cell Res. 2019 Aug;39:101489
pubmed: 31277043
Int J Mol Sci. 2020 Feb 08;21(3):
pubmed: 32046263
J Orthop Res. 2006 Mar;24(3):481-90
pubmed: 16453340
J Biomed Mater Res B Appl Biomater. 2012 Apr;100(3):752-8
pubmed: 22323314
J Tissue Eng Regen Med. 2020 Jan;14(1):135-146
pubmed: 31622052
Cell Death Discov. 2017 Jul 17;3:17038
pubmed: 28725488
Am J Sports Med. 2005 Nov;33(11):1751-67
pubmed: 16230470
Int J Mol Sci. 2020 Jan 16;21(2):
pubmed: 31963350
Int J Mol Sci. 2019 Sep 19;20(18):
pubmed: 31546928
Biomed Res Int. 2015;2015:790804
pubmed: 26229962
PLoS One. 2011;6(11):e27209
pubmed: 22069500
Biomaterials. 2015 May;51:43-50
pubmed: 25770996
Knee Surg Sports Traumatol Arthrosc. 2006 Mar;14(3):204-13
pubmed: 16235056
J Orthop Res. 2010 Sep;28(9):1170-7
pubmed: 20187116
Proc Natl Acad Sci U S A. 2016 Jul 12;113(28):7840-5
pubmed: 27370800
J Biomater Sci Polym Ed. 2015;26(16):1085-99
pubmed: 26300365
J Orthop Sci. 2010 Jul;15(4):569-77
pubmed: 20721727
J Cell Sci. 2005 Mar 1;118(Pt 5):937-49
pubmed: 15713747
Arch Oral Biol. 2012 Dec;57(12):1602-8
pubmed: 22871357
Tissue Eng Part A. 2017 Aug;23(15-16):811-822
pubmed: 28401805
J Bone Joint Surg Br. 1992 May;74(3):403-5
pubmed: 1587888
Development. 2017 Dec 1;144(23):4271-4283
pubmed: 29183940
Stem Cells Int. 2016;2016:7342379
pubmed: 27630718
J Bone Miner Metab. 2014 Nov;32(6):671-82
pubmed: 24362490
Biochem Biophys Res Commun. 2019 Jan 15;508(3):889-893
pubmed: 30538046
Acta Bioeng Biomech. 2013;15(3):71-9
pubmed: 24215499
Biol Trace Elem Res. 2020 Jan;193(1):195-203
pubmed: 30887282
Cell Tissue Res. 2021 Jun;384(3):789
pubmed: 33977325
Am J Sports Med. 2006 Sep;34(9):1512-32
pubmed: 16905673
Ann Chir. 1995;49(8):768-74
pubmed: 8561433
Biophys J. 2016 Jul 12;111(1):50-6
pubmed: 27410733
J Orthop Res. 1998 Jul;16(4):406-13
pubmed: 9747780
J Tissue Eng. 2014 Nov 06;5:2041731414556561
pubmed: 25396042
J Orthop Res. 2002 Jul;20(4):827-33
pubmed: 12168674
Med Sci Sports Exerc. 1995 Jun;27(6):844-51
pubmed: 7658945
Biochem Biophys Res Commun. 2015 Sep 18;465(2):281-6
pubmed: 26260322
Cell Death Dis. 2017 Oct 12;8(10):e3116
pubmed: 29022912
Muscles Ligaments Tendons J. 2018 Jan 10;7(3):411-417
pubmed: 29387633
Eur J Cell Biol. 2006 Nov;85(11):1145-54
pubmed: 16859807
J Biomech. 2007;40(9):2029-36
pubmed: 17097666
J Biomech. 2018 Sep 10;78:134-142
pubmed: 30107900
Cell Motil Cytoskeleton. 2007 Feb;64(2):121-30
pubmed: 17183550
Histochem Cell Biol. 2004 Sep;122(3):219-28
pubmed: 15338228
Anat Embryol (Berl). 1999 Sep;200(3):325-34
pubmed: 10463347
J Mol Med (Berl). 2006 Nov;84(11):901-10
pubmed: 16972087
Histochem Cell Biol. 2015 Mar;143(3):289-300
pubmed: 25256666
Nat Rev Mol Cell Biol. 2002 May;3(5):349-63
pubmed: 11988769
Biomaterials. 2015 Nov;69:99-109
pubmed: 26283157
J Bone Joint Surg Am. 2014 Apr 16;96(8):685-94
pubmed: 24740666
Am J Orthod Dentofacial Orthop. 2014 Apr;145(4):469-79
pubmed: 24703285
Am J Sports Med. 2005 Oct;33(10):1579-602
pubmed: 16199611
J Cell Physiol. 2000 Mar;182(3):311-22
pubmed: 10653597
Cell Struct Funct. 2002 Jun;27(3):139-44
pubmed: 12207044
Clin Orthop Relat Res. 1998 Aug;(353):247-55
pubmed: 9728181
Sports Med Arthrosc Rehabil Ther Technol. 2010 Aug 20;2:20
pubmed: 20727171
FASEB J. 2018 Sep;32(9):4804-4814
pubmed: 29596022
Eur Spine J. 2011 Sep;20(9):1459-65
pubmed: 21442291
World J Orthop. 2015 Jan 18;6(1):127-36
pubmed: 25621217
J Cell Sci. 2003 Mar 1;116(Pt 5):857-66
pubmed: 12571283
J Biomech. 2013 Feb 1;46(3):478-83
pubmed: 23178040
Sci Rep. 2017 Sep 14;7(1):11527
pubmed: 28912559