An ELIME assay for hepatitis A virus detection.

Biological Assay Hepatitis A virus / genetics Real-Time Polymerase Chain Reaction Reverse Transcriptase Polymerase Chain Reaction Sensitivity and Specificity
ELIME Food Hepatitis A virus Polydopamine-modified magnetic nanobeads Screen printed electrodes

Journal

Talanta
ISSN: 1873-3573
Titre abrégé: Talanta
Pays: Netherlands
ID NLM: 2984816R

Informations de publication

Date de publication:
01 Nov 2021
Historique:
received: 10 03 2021
revised: 24 06 2021
accepted: 26 06 2021
entrez: 8 8 2021
pubmed: 9 8 2021
medline: 11 8 2021
Statut: ppublish

Résumé

An Enzyme Linked ImmunoMagnetic Electrochemical assay (ELIME) was developed for the detection of the hepatitis A virus (HAV). This system is based on the use of new polydopamine-modified magnetic nanobeads as solid support for the immunochemical chain, and an array of 8 screen-printed electrodes as a sensing platform. Enzymatic-by-product is quickly measured by differential pulse voltammetry. For this purpose, all analytical parameters were optimized; in particular, different blocking reagents were evaluated in order to minimize the nonspecific interaction of bioreagents. Using the ELIME assays, a quantitative determination of HAV can be achieved with a detection limit of 1·10

Identifiants

DOI: 10.1016/j.talanta.2021.122672 PMID: 34364473
pubmed: 34364473
pii: S0039-9140(21)00593-2
doi: 10.1016/j.talanta.2021.122672
pii:
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

122672

Informations de copyright

Copyright © 2021 Elsevier B.V. All rights reserved.

Auteurs

Laura Micheli (L)

Department of Chemical Sciences and Technologies, University of Rome "Tor Vergata", Via della Ricerca Scientifica, 00133, Rome, Italy; Consorzio Interuniversitario Biostrutture e Biosistemi "INBB", Viale Medaglie d'Oro 305, 00136, Rome, Italy. Electronic address: laura.micheli@uniroma2.it.

Andrea Fasoli (A)

Department of Chemical Sciences and Technologies, University of Rome "Tor Vergata", Via della Ricerca Scientifica, 00133, Rome, Italy.

Aisha Attar (A)

Faculty of Sciences and Techniques, Hassan II University of Casablanca, BP 146, Mohammedia, 20650, Morocco; Department of Pharmacology, College of Medicine, The University of Illinois at Chicago, 835 S Wolcott Ave E403 (MC868), Chicago, IL, 60612, USA.

Domenica T Donia (DT)

Department of Experimental Medicine and Surgery, University of Roma "Tor Vergata", Via Montpellier, 1, 00133, Roma, Italy.

Maurizio Divizia (M)

Department of Experimental Medicine and Surgery, University of Roma "Tor Vergata", Via Montpellier, 1, 00133, Roma, Italy.

Aziz Amine (A)

Faculty of Sciences and Techniques, Hassan II University of Casablanca, BP 146, Mohammedia, 20650, Morocco.

Giuseppe Palleschi (G)

Department of Chemical Sciences and Technologies, University of Rome "Tor Vergata", Via della Ricerca Scientifica, 00133, Rome, Italy; Consorzio Interuniversitario Biostrutture e Biosistemi "INBB", Viale Medaglie d'Oro 305, 00136, Rome, Italy.

Pedro A Salazar Carballo (PA)

Neurochemistry and Neuroimaging Group, (Laboratory of Sensors, Biosensors and Materials) Faculty of Medical Sciences, University of La Laguna, Campus de Ofra s/n, 38071, La Laguna, Tenerife, Spain.

Danila Moscone (D)

Department of Chemical Sciences and Technologies, University of Rome "Tor Vergata", Via della Ricerca Scientifica, 00133, Rome, Italy; Consorzio Interuniversitario Biostrutture e Biosistemi "INBB", Viale Medaglie d'Oro 305, 00136, Rome, Italy.

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Classifications MeSH

questionsmedicales.fr › Techniques d'investigation › Dosage biologique › An ELIME assay for hepatitis A virus detection.
questionsmedicales.fr › Virus › Virus à ARN › Virus à ARN à brin positif › Picornaviridae › Hepatovirus › Virus de l'hépatite A › An ELIME assay for hepatitis A virus detection.
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Techniques d'amplification d'acides nucléiques › Réaction de polymérisation en chaîne › Réaction de polymérisation en chaine en temps réel › An ELIME assay for hepatitis A virus detection.
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Techniques d'amplification d'acides nucléiques › Réaction de polymérisation en chaîne › RT-PCR › An ELIME assay for hepatitis A virus detection.
questionsmedicales.fr › Environnement et santé publique › Santé publique › Méthodes épidémiologiques › Statistiques comme sujet › Sensibilité et spécificité › An ELIME assay for hepatitis A virus detection.