The lane-switch mechanism for nucleosome repositioning by DNA translocase.
Journal
Nucleic acids research
ISSN: 1362-4962
Titre abrégé: Nucleic Acids Res
Pays: England
ID NLM: 0411011
Informations de publication
Date de publication:
20 09 2021
20 09 2021
Historique:
accepted:
26
07
2021
revised:
18
07
2021
received:
20
04
2021
pubmed:
9
8
2021
medline:
9
11
2021
entrez:
8
8
2021
Statut:
ppublish
Résumé
Translocases such as DNA/RNA polymerases, replicative helicases, and exonucleases are involved in eukaryotic DNA transcription, replication, and repair. Since eukaryotic genomic DNA wraps around histone octamers and forms nucleosomes, translocases inevitably encounter nucleosomes. A previous study has shown that a nucleosome repositions downstream when a translocase collides with the nucleosome. However, the molecular mechanism of the downstream repositioning remains unclear. In this study, we identified the lane-switch mechanism for downstream repositioning with molecular dynamics simulations and validated it with restriction enzyme digestion assays and deep sequencing assays. In this mechanism, after a translocase unwraps nucleosomal DNA up to the site proximal to the dyad, the remaining wrapped DNA switches its binding lane to that vacated by the unwrapping, and the downstream DNA rewraps, completing downstream repositioning. This mechanism may have broad implications for transcription through nucleosomes, histone recycling, and nucleosome remodeling.
Identifiants
pubmed: 34365508
pii: 6345465
doi: 10.1093/nar/gkab664
pmc: PMC8450081
doi:
Substances chimiques
Histones
0
Nucleosomes
0
DNA-Directed RNA Polymerases
EC 2.7.7.6
Exodeoxyribonucleases
EC 3.1.-
DNA Helicases
EC 3.6.4.-
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
9066-9076Informations de copyright
© The Author(s) 2021. Published by Oxford University Press on behalf of Nucleic Acids Research.
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