Polyacrylate-GnRH Peptide Conjugate as an Oral Contraceptive Vaccine Candidate.
GnRH
PADRE
antibody
immunocontraceptive
oral vaccine
pig T helper
poly(methylacrylate)
Journal
Pharmaceutics
ISSN: 1999-4923
Titre abrégé: Pharmaceutics
Pays: Switzerland
ID NLM: 101534003
Informations de publication
Date de publication:
15 Jul 2021
15 Jul 2021
Historique:
received:
09
06
2021
revised:
30
06
2021
accepted:
12
07
2021
entrez:
10
8
2021
pubmed:
11
8
2021
medline:
11
8
2021
Statut:
epublish
Résumé
Contraceptive vaccines are designed to elicit immune responses against major components of animal reproductive systems. These vaccines, which are most commonly administered via injection, typically target gonadotropin-releasing hormone (GnRH). However, the need to restrain animals for treatment limits the field applications of injectable vaccines. Oral administration would broaden vaccine applicability. We explored contraceptive vaccine candidates composed of GnRH peptide hormone, universal T helper PADRE (P), and a poly(methylacrylate) (PMA)-based delivery system. When self-assembled into nanoparticles, PMA-P-GnRH induced the production of high IgG titers after subcutaneous and oral administration in mice. PADRE was then replaced with pig T helper derived from the swine flu virus, and the vaccine was tested in pigs. High levels of systemic antibodies were produced in pigs after both injection and oral administration of the vaccine. In conclusion, we developed a simple peptide-polymer conjugate that shows promise as an effective, adjuvant-free, oral GnRH-based contraceptive vaccine.
Identifiants
pubmed: 34371772
pii: pharmaceutics13071081
doi: 10.3390/pharmaceutics13071081
pmc: PMC8308917
pii:
doi:
Types de publication
Journal Article
Langues
eng
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