Single-Molecule Enzymology for Diagnostics: Profiling Alkaline Phosphatase Activity in Clinical Samples.


Journal

Chembiochem : a European journal of chemical biology
ISSN: 1439-7633
Titre abrégé: Chembiochem
Pays: Germany
ID NLM: 100937360

Informations de publication

Date de publication:
05 01 2022
Historique:
revised: 06 08 2021
received: 20 07 2021
pubmed: 11 8 2021
medline: 22 2 2022
entrez: 10 8 2021
Statut: ppublish

Résumé

Enzymes can be used as biomarkers for a variety of diseases. However, profiling enzyme activity in clinical samples is challenging due to the heterogeneity in enzyme activity, and the low abundance of the target enzyme in biofluids. Single-molecule methods can overcome these challenges by providing information on the distribution of enzyme activities in a sample. Here, we describe the concept of using the single-molecule enzymology (SME) method to analyze enzymatic activity in clinical samples. We present recent work focused on measuring alkaline phosphatase isotypes in serum samples using SME. Future work will involve improving and simplifying this technology, and applying it to other enzymes for diagnostics.

Identifiants

pubmed: 34375495
doi: 10.1002/cbic.202100358
doi:

Substances chimiques

Biomarkers 0
Alkaline Phosphatase EC 3.1.3.1

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

e202100358

Informations de copyright

© 2021 Wiley-VCH GmbH.

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Auteurs

Tal Gilboa (T)

Department of Pathology, Brigham and Women's Hospital, Boston, MA, USA.
Wyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, USA.
Harvard Medical School, Boston, MA, USA.

Alana F Ogata (AF)

Department of Pathology, Brigham and Women's Hospital, Boston, MA, USA.
Wyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, USA.
Harvard Medical School, Boston, MA, USA.

David R Walt (DR)

Department of Pathology, Brigham and Women's Hospital, Boston, MA, USA.
Wyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, USA.
Harvard Medical School, Boston, MA, USA.

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