Bisection of the X chromosome disrupts the initiation of chromosome silencing during meiosis in Caenorhabditis elegans.
Animals
Caenorhabditis elegans
/ genetics
Caenorhabditis elegans Proteins
/ genetics
Cell Cycle Proteins
/ genetics
Chromatin
/ genetics
Chromosome Pairing
/ genetics
Female
Gene Silencing
Germ Cells
/ cytology
Histones
/ metabolism
In Situ Hybridization, Fluorescence
Male
Meiosis
/ genetics
Microscopy, Fluorescence
Transcription, Genetic
X Chromosome
/ genetics
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
10 08 2021
10 08 2021
Historique:
received:
12
06
2020
accepted:
01
07
2021
entrez:
11
8
2021
pubmed:
12
8
2021
medline:
24
8
2021
Statut:
epublish
Résumé
During meiosis, gene expression is silenced in aberrantly unsynapsed chromatin and in heterogametic sex chromosomes. Initiation of sex chromosome silencing is disrupted in meiocytes with sex chromosome-autosome translocations. To determine whether this is due to aberrant synapsis or loss of continuity of sex chromosomes, we engineered Caenorhabditis elegans nematodes with non-translocated, bisected X chromosomes. In early meiocytes of mutant males and hermaphrodites, X segments are enriched with euchromatin assembly markers and active RNA polymerase II staining, indicating active transcription. Analysis of RNA-seq data showed that genes from the X chromosome are upregulated in gonads of mutant worms. Contrary to previous models, which predicted that any unsynapsed chromatin is silenced during meiosis, our data indicate that unsynapsed X segments are transcribed. Therefore, our results suggest that sex chromosome chromatin has a unique character that facilitates its meiotic expression when its continuity is lost, regardless of whether or not it is synapsed.
Identifiants
pubmed: 34376665
doi: 10.1038/s41467-021-24815-0
pii: 10.1038/s41467-021-24815-0
pmc: PMC8355143
doi:
Substances chimiques
Caenorhabditis elegans Proteins
0
Cell Cycle Proteins
0
Chromatin
0
HIM-8 protein, C elegans
0
Histones
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
4802Informations de copyright
© 2021. The Author(s).
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