CRISPR/Cas9-engineered inducible gametocyte producer lines as a valuable tool for Plasmodium falciparum malaria transmission research.
Animals
Anopheles
/ parasitology
CRISPR-Cas Systems
Cells, Cultured
Erythrocytes
/ parasitology
Hepatocytes
/ cytology
Host-Parasite Interactions
Humans
Malaria, Falciparum
/ blood
Microscopy, Fluorescence
Mosquito Vectors
/ parasitology
Plasmodium falciparum
/ genetics
Spores, Protozoan
/ genetics
Sporozoites
/ genetics
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
10 08 2021
10 08 2021
Historique:
received:
05
10
2020
accepted:
08
07
2021
entrez:
11
8
2021
pubmed:
12
8
2021
medline:
24
8
2021
Statut:
epublish
Résumé
The malaria parasite Plasmodium falciparum replicates inside erythrocytes in the blood of infected humans. During each replication cycle, a small proportion of parasites commits to sexual development and differentiates into gametocytes, which are essential for parasite transmission via the mosquito vector. Detailed molecular investigation of gametocyte biology and transmission has been hampered by difficulties in generating large numbers of these highly specialised cells. Here, we engineer P. falciparum NF54 inducible gametocyte producer (iGP) lines for the routine mass production of synchronous gametocytes via conditional overexpression of the sexual commitment factor GDV1. NF54/iGP lines consistently achieve sexual commitment rates of 75% and produce viable gametocytes that are transmissible by mosquitoes. We also demonstrate that further genetic engineering of NF54/iGP parasites is a valuable tool for the targeted exploration of gametocyte biology. In summary, we believe the iGP approach developed here will greatly expedite basic and applied malaria transmission stage research.
Identifiants
pubmed: 34376675
doi: 10.1038/s41467-021-24954-4
pii: 10.1038/s41467-021-24954-4
pmc: PMC8355313
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
4806Informations de copyright
© 2021. The Author(s).
Références
Sci Rep. 2019 Oct 10;9(1):14595
pubmed: 31601834
Nat Methods. 2017 Jan;14(1):53-56
pubmed: 27869816
Parasit Vectors. 2019 May 6;12(1):206
pubmed: 31060594
Nat Commun. 2020 Mar 20;11(1):1503
pubmed: 32198457
PLoS Pathog. 2012;8(10):e1002964
pubmed: 23093935
Cell Microbiol. 2015 Feb;17(2):207-25
pubmed: 25224798
Malar J. 2010 Jul 08;9:194
pubmed: 20615232
Mol Biochem Parasitol. 1995 Nov;74(2):143-56
pubmed: 8719156
J Cell Sci. 2012 Apr 15;125(Pt 8):2053-63
pubmed: 22328505
Mol Biochem Parasitol. 1994 May;65(1):11-22
pubmed: 7935618
Methods Mol Biol. 2013;923:17-25
pubmed: 22990768
Nature. 2014 Mar 13;507(7491):253-257
pubmed: 24572359
Int J Parasitol. 2010 Jan;40(1):109-21
pubmed: 19765590
Nat Protoc. 2016 May;11(5):976-92
pubmed: 27123949
Am J Trop Med Hyg. 2003 Apr;68(4):403-9
pubmed: 12875287
BMC Genomics. 2014 May 08;15:347
pubmed: 24885191
Genome Biol. 2012 Nov 26;13(11):R108
pubmed: 23181666
Nature. 2017 Nov 2;551(7678):95-99
pubmed: 29094698
PLoS Pathog. 2015 May 07;11(5):e1004815
pubmed: 25951195
Malar J. 2010 May 24;9:136
pubmed: 20497536
Parasitology. 1989 Apr;98 Pt 2:165-73
pubmed: 2668861
Nucleic Acids Res. 2016 Jul 27;44(13):6087-101
pubmed: 27298255
Nat Methods. 2006 Aug;3(8):615-21
pubmed: 16862136
Curr Opin Cell Biol. 2019 Jun;58:126-133
pubmed: 31063899
Am J Trop Med Hyg. 1999 Jan;60(1):7-13
pubmed: 9988315
Science. 2003 Sep 12;301(5639):1503-8
pubmed: 12893887
Cell Host Microbe. 2014 Aug 13;16(2):165-176
pubmed: 25121746
Cell. 2017 Dec 14;171(7):1532-1544.e15
pubmed: 29129376
PLoS One. 2013 Aug 30;8(8):e73783
pubmed: 24023691
Annu Rev Microbiol. 2018 Sep 08;72:501-519
pubmed: 29975590
Nature. 2002 Oct 3;419(6906):520-6
pubmed: 12368866
Nature. 2014 Mar 13;507(7491):248-52
pubmed: 24572369
Nat Commun. 2019 May 13;10(1):2140
pubmed: 31086187
Nature. 2002 Oct 3;419(6906):537-42
pubmed: 12368870
Nat Struct Mol Biol. 2011 Mar;18(3):359-63
pubmed: 21317896
BMC Med. 2017 Sep 13;15(1):168
pubmed: 28903777
Mol Biochem Parasitol. 2007 Jul;154(1):119-23
pubmed: 17521751
Cell Host Microbe. 2018 Mar 14;23(3):407-420.e8
pubmed: 29503181
BMC Genomics. 2019 Dec 3;20(1):920
pubmed: 31795940
Nat Commun. 2021 Jun 21;12(1):3820
pubmed: 34155201
Nat Commun. 2018 May 15;9(1):1910
pubmed: 29765020
Malar J. 2017 Jan 13;16(1):26
pubmed: 28086874
Mol Cell Proteomics. 2014 Oct;13(10):2705-24
pubmed: 25056935
Blood. 2016 Jun 16;127(24):e42-53
pubmed: 27136945
PLoS Pathog. 2017 Oct 6;13(10):e1006659
pubmed: 28985225
PLoS One. 2016 Jun 22;11(6):e0157873
pubmed: 27332706
J Infect Dis. 2014 Nov 1;210(9):1456-63
pubmed: 24829466
Sci Rep. 2013 Dec 04;3:3418
pubmed: 24301557
Mol Biochem Parasitol. 2005 Sep;143(1):67-79
pubmed: 16005087
Parasitology. 2013 Dec;140(14):1718-34
pubmed: 23953486
BMC Genomics. 2011 Nov 30;12:587
pubmed: 22129310
F1000Res. 2019 Apr 3;8:
pubmed: 31001417
Trans R Soc Trop Med Hyg. 1971;65(5):549-59
pubmed: 5003557
Mol Biochem Parasitol. 2019 Apr;229:47-52
pubmed: 30831155
J Parasitol. 1979 Jun;65(3):418-20
pubmed: 383936
Nat Protoc. 2016 Sep;11(9):1668-80
pubmed: 27560172
Bull World Health Organ. 1979;57 Suppl 1:37-52
pubmed: 397008
Mol Cell Proteomics. 2010 Jul;9(7):1437-48
pubmed: 20332084
Nucleic Acids Res. 2016 Jul 8;44(W1):W272-6
pubmed: 27185894
Science. 2018 Mar 16;359(6381):1259-1263
pubmed: 29590075
Front Microbiol. 2019 Nov 08;10:2652
pubmed: 31787966
Parasitology. 1982 Feb;84(1):1-11
pubmed: 7038594
Nucleic Acids Res. 2017 Jul 27;45(13):7825-7840
pubmed: 28531310
Infect Dis Poverty. 2016 Jun 09;5(1):61
pubmed: 27282148
Parasitology. 1986 Oct;93 ( Pt 2):263-74
pubmed: 3537921
Cold Spring Harb Perspect Med. 2017 Mar 1;7(3):
pubmed: 27836912
Sci Adv. 2020 Jun 10;6(24):eaaz5057
pubmed: 32577509
J Parasitol Res. 2012;2012:927148
pubmed: 22523643
PLoS Med. 2017 Nov 30;14(11):e1002456
pubmed: 29190300
Cell Host Microbe. 2014 Aug 13;16(2):177-186
pubmed: 25121747
Blood. 2012 Jun 14;119(24):e172-80
pubmed: 22517905
Eukaryot Cell. 2013 May;12(5):697-702
pubmed: 23475702
Malar J. 2015 May 22;14:213
pubmed: 25994518
Cell. 2005 Jun 3;121(5):675-87
pubmed: 15935755
Nat Microbiol. 2018 Nov;3(11):1206-1213
pubmed: 30177743
Sci Transl Med. 2014 Jul 9;6(244):244re5
pubmed: 25009232
Parasitology. 1990 Apr;100 Pt 2:191-200
pubmed: 2189114
Front Immunol. 2018 Dec 11;9:2748
pubmed: 30619241
Mol Biol Evol. 2012 Sep;29(9):2113-32
pubmed: 22389454
Mol Cell Proteomics. 2017 Apr;16(4):537-551
pubmed: 28126901
Parasitology. 2000 Nov;121 Pt 5:465-71
pubmed: 11128797
J Cell Sci. 2013 Jul 15;126(Pt 14):3055-69
pubmed: 23687383
Malar J. 2016 Sep 09;15:463
pubmed: 27612458
Wellcome Open Res. 2018 Jun 13;3:70
pubmed: 30320226
Nat Microbiol. 2019 Jan;4(1):144-154
pubmed: 30478286
Cell Host Microbe. 2016 Jan 13;19(1):114-26
pubmed: 26749441
FEMS Microbiol Rev. 2019 Jul 1;43(4):401-414
pubmed: 31220244
Nat Methods. 2009 May;6(5):343-5
pubmed: 19363495
Malar J. 2020 May 25;19(1):192
pubmed: 32450861
Cell Microbiol. 2011 Jul;13(7):967-77
pubmed: 21501361
Proc R Soc Lond B Biol Sci. 1978 Jun 5;201(1145):375-99
pubmed: 27809
Proc Natl Acad Sci U S A. 2016 Apr 26;113(17):4800-5
pubmed: 27071094
Cell Microbiol. 2012 Jul;14(7):983-93
pubmed: 22417683
Parasit Vectors. 2017 May 2;10(1):216
pubmed: 28464929
Nat Methods. 2007 Dec;4(12):1007-9
pubmed: 17994030
Nat Protoc. 2015 Aug;10(8):1131-42
pubmed: 26134953
Sci Rep. 2018 Jul 26;8(1):11249
pubmed: 30050042