Hyperspectral image processing for the identification and quantification of lentiviral particles in fluid samples.

HEK293 Cells Humans Image Processing, Computer-Assisted / methods Lentivirus / isolation & purification Lentivirus Infections / diagnosis Molecular Diagnostic Techniques / methods Point-of-Care Systems Saliva / virology Sensitivity and Specificity Spectroscopy, Near-Infrared / methods

Journal

Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288

Informations de publication

Date de publication:
10 08 2021
Historique:
received: 04 06 2021
accepted: 30 07 2021
entrez: 11 8 2021
pubmed: 12 8 2021
medline: 21 8 2021
Statut: epublish

Résumé

Optical spectroscopic techniques have been commonly used to detect the presence of biofilm-forming pathogens (bacteria and fungi) in the agro-food industry. Recently, near-infrared (NIR) spectroscopy revealed that it is also possible to detect the presence of viruses in animal and vegetal tissues. Here we report a platform based on visible and NIR (VNIR) hyperspectral imaging for non-contact, reagent free detection and quantification of laboratory-engineered viral particles in fluid samples (liquid droplets and dry residue) using both partial least square-discriminant analysis and artificial feed-forward neural networks. The detection was successfully achieved in preparations of phosphate buffered solution and artificial saliva, with an equivalent pixel volume of 4 nL and lowest concentration of 800 TU·[Formula: see text]L

Identifiants

DOI: 10.1038/s41598-021-95756-3 PMID: 34376765 PMC: PMC8355230
pubmed: 34376765
doi: 10.1038/s41598-021-95756-3
pii: 10.1038/s41598-021-95756-3
pmc: PMC8355230
doi:

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

16201

Subventions

Organisme : Instituto de Salud Carlos III
ID : COV20-00080
Organisme : Instituto de Salud Carlos III
ID : COV20-00080
Organisme : Instituto de Salud Carlos III
ID : COV20-00080
Organisme : Instituto de Salud Carlos III
ID : COV20-00080
Organisme : Instituto de Salud Carlos III
ID : COV20-00080
Organisme : Instituto de Salud Carlos III
ID : COV20-00080
Organisme : Instituto de Salud Carlos III
ID : COV20-00080
Organisme : Instituto de Salud Carlos III
ID : COV20-00080
Organisme : Instituto de Salud Carlos III
ID : COV20-00080
Organisme : Instituto de Salud Carlos III
ID : COV20-00080
Organisme : Instituto de Salud Carlos III
ID : COV20-00080
Organisme : Instituto de Salud Carlos III
ID : COV20-00080
Organisme : Instituto de Salud Carlos III
ID : COV20-00080
Organisme : Instituto de Salud Carlos III
ID : COV20-00080
Organisme : Instituto de Salud Carlos III
ID : COV20-00080
Organisme : Instituto de Salud Carlos III
ID : COV20-00080
Organisme : Instituto de Salud Carlos III
ID : COV20-00080
Organisme : Instituto de Salud Carlos III
ID : COV20-00080
Organisme : Instituto de Salud Carlos III
ID : COV20-00080
Organisme : Instituto de Salud Carlos III
ID : COV20-00080
Organisme : Instituto de Salud Carlos III
ID : COV20-00173
Organisme : Instituto de Salud Carlos III
ID : COV20-00173
Organisme : Instituto de Salud Carlos III
ID : COV20-00080
Organisme : Instituto de Salud Carlos III
ID : COV20-00080
Organisme : Instituto de Salud Carlos III
ID : COV20-00080
Organisme : Instituto de Salud Carlos III
ID : COV20-00080
Organisme : Ministerio de Ciencia e Innovación
ID : EQC2019-006240-P
Organisme : Ministerio de Ciencia e Innovación
ID : EQC2019-006240-P
Organisme : Ministerio de Ciencia e Innovación
ID : EQC2019-006240-P
Organisme : Ministerio de Ciencia e Innovación
ID : EQC2019-006240-P
Organisme : Ministerio de Ciencia e Innovación
ID : EQC2019-006240-P
Organisme : Ministerio de Ciencia e Innovación
ID : EQC2019-006240-P
Organisme : Ministerio de Ciencia e Innovación
ID : EQC2019-006240-P
Organisme : Ministerio de Ciencia e Innovación
ID : EQC2019-006240-P
Organisme : Ministerio de Ciencia e Innovación
ID : EQC2019-006240-P
Organisme : Ministerio de Ciencia e Innovación
ID : EQC2019-006240-P
Organisme : Spanish National Research Agency
ID : RTI2018-094465-J

Informations de copyright

© 2021. The Author(s).

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Auteurs

Emilio Gomez-Gonzalez (E)

Department of Applied Physics III, School of Engineering, Universidad de Sevilla, Camino de los Descubrimientos s/n, 41092, Sevilla, Spain. egomez@us.es.
Institute of Biomedicine of Seville, 41013, Sevilla, Spain. egomez@us.es.

Beatriz Fernandez-Muñoz (B)

Unidad de Producción y Reprogramación Celular (UPRC), Red Andaluza de Diseño y Traslación de Terapias Avanzadas, 41092, Sevilla, Spain.

Alejandro Barriga-Rivera (A)

Department of Applied Physics III, School of Engineering, Universidad de Sevilla, Camino de los Descubrimientos s/n, 41092, Sevilla, Spain.
School of Biomedical Engineering, The University of Sydney, Sydney, NSW, 2006, Australia.

Jose Manuel Navas-Garcia (JM)

EOD-CBRN Group, Spanish National Police, 41011, Sevilla, Spain.

Isabel Fernandez-Lizaranzu (I)

Department of Applied Physics III, School of Engineering, Universidad de Sevilla, Camino de los Descubrimientos s/n, 41092, Sevilla, Spain.
Institute of Biomedicine of Seville, 41013, Sevilla, Spain.

Francisco Javier Munoz-Gonzalez (FJ)

Department of Applied Physics III, School of Engineering, Universidad de Sevilla, Camino de los Descubrimientos s/n, 41092, Sevilla, Spain.

Ruben Parrilla-Giraldez (R)

Technology and Innovation Centre, Universidad de Sevilla, 41012, Sevilla, Spain.

Desiree Requena-Lancharro (D)

Department of Applied Physics III, School of Engineering, Universidad de Sevilla, Camino de los Descubrimientos s/n, 41092, Sevilla, Spain.

Manuel Guerrero-Claro (M)

Department of Applied Physics III, School of Engineering, Universidad de Sevilla, Camino de los Descubrimientos s/n, 41092, Sevilla, Spain.

Pedro Gil-Gamboa (P)

Department of Applied Physics III, School of Engineering, Universidad de Sevilla, Camino de los Descubrimientos s/n, 41092, Sevilla, Spain.

Cristina Rosell-Valle (C)

Institute of Biomedicine of Seville, 41013, Sevilla, Spain.
Unidad de Producción y Reprogramación Celular (UPRC), Red Andaluza de Diseño y Traslación de Terapias Avanzadas, 41092, Sevilla, Spain.

Carmen Gomez-Gonzalez (C)

Service of Intensive Care, University Hospital 'Virgen del Rocio', 41013, Sevilla, Spain.

Maria Jose Mayorga-Buiza (MJ)

Institute of Biomedicine of Seville, 41013, Sevilla, Spain.
Service of Anaesthesiology, University Hospital 'Virgen del Rocio', 41013, Sevilla, Spain.

Maria Martin-Lopez (M)

Institute of Biomedicine of Seville, 41013, Sevilla, Spain.
Unidad de Producción y Reprogramación Celular (UPRC), Red Andaluza de Diseño y Traslación de Terapias Avanzadas, 41092, Sevilla, Spain.

Olga Muñoz (O)

Instituto de Astrofísica de Andalucía, CSIC, 18008, Granada, Spain.

Juan Carlos Gomez Martin (JCG)

Instituto de Astrofísica de Andalucía, CSIC, 18008, Granada, Spain.

Maria Isabel Relimpio Lopez (MIR)

Department of Ophthalmology, University Hospital 'Virgen Macarena', 41009, Sevilla, Spain.
OftaRed, Institute of Health 'Carlos III', 28029, Madrid, Spain.

Jesus Aceituno-Castro (J)

Instituto de Astrofísica de Andalucía, CSIC, 18008, Granada, Spain.
Centro Astronomico Hispano Alemán, 04550, Almeria, Spain.

Manuel A Perales-Esteve (MA)

Department of Electronic Engineering, School of Engineering, Universidad de Sevilla, 41092, Sevilla, Spain.

Antonio Puppo-Moreno (A)

Service of Intensive Care, University Hospital 'Virgen del Rocio', 41013, Sevilla, Spain.

Francisco Jose Garcia Cozar (FJG)

Department of Biomedicine, Biotechnology and Public Health, University of Cadiz, 11003, Cadiz, Spain.

Lucia Olvera-Collantes (L)

Instituto de Investigación E Innovación Biomedica de Cádiz (INIBICA), 11009, Cadiz, Spain.

Silvia de Los Santos-Trigo (S)

Corporación Tecnológica de Andalucía, 41092, Sevilla, Spain.

Emilia Gomez (E)

Joint Research Centre, European Commission, 41092, Sevilla, Spain.

Rosario Sanchez Pernaute (RS)

Unidad de Producción y Reprogramación Celular (UPRC), Red Andaluza de Diseño y Traslación de Terapias Avanzadas, 41092, Sevilla, Spain.

Javier Padillo-Ruiz (J)

Institute of Biomedicine of Seville, 41013, Sevilla, Spain.
Department of General Surgery, University Hospital 'Virgen del Rocío', 41013, Sevilla, Spain.

Javier Marquez-Rivas (J)

Institute of Biomedicine of Seville, 41013, Sevilla, Spain.
Service of Neurosurgery, University Hospital 'Virgen del Rocío', 41013, Sevilla, Spain.
Centre for Advanced Neurology, 41013, Sevilla, Spain.

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Classifications MeSH

questionsmedicales.fr › Cellules › Cellules épithéliales › Cellules HEK293 › Hyperspectral image processing for the...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Hyperspectral image processing for the...
questionsmedicales.fr › Sciences de l'information › Méthodologies informatiques › Traitement d'image par ordinateur › Hyperspectral image processing for the...
questionsmedicales.fr › Virus › Virus à ARN › Retroviridae › Lentivirus › Hyperspectral image processing for the...
questionsmedicales.fr › Infection › Maladies virales › Infections à virus à ARN › Infections à Retroviridae › Infections à lentivirus › Hyperspectral image processing for the...
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Techniques de diagnostic moléculaire › Hyperspectral image processing for the...
questionsmedicales.fr › Administration des services de santé › Gestion des soins aux patients › Systèmes automatisés lit malade › Hyperspectral image processing for the...
questionsmedicales.fr › Liquides et sécrétions biologiques › Sécrétions corporelles › Salive › Hyperspectral image processing for the...
questionsmedicales.fr › Environnement et santé publique › Santé publique › Méthodes épidémiologiques › Statistiques comme sujet › Sensibilité et spécificité › Hyperspectral image processing for the...
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Analyse spectrale › Spectroscopie proche infrarouge › Hyperspectral image processing for the...