Honeybee Exposure to Veterinary Drugs: How Is the Gut Microbiota Affected?
Bombilactobacillus
Lactobacillus
NGS
antibiotic resistance
bifidobacteria
honeybees
microbiota
next-generation sequencing
sulphonamides
tetracyclines
tylosin
Journal
Microbiology spectrum
ISSN: 2165-0497
Titre abrégé: Microbiol Spectr
Pays: United States
ID NLM: 101634614
Informations de publication
Date de publication:
03 09 2021
03 09 2021
Historique:
pubmed:
12
8
2021
medline:
30
12
2021
entrez:
11
8
2021
Statut:
ppublish
Résumé
Several studies have outlined that a balanced gut microbiota offers metabolic and protective functions supporting honeybee health and performance. The present work contributes to increasing knowledge on the impact on the honeybee gut microbiota of the three most common veterinary drugs (oxytetracycline, sulfonamides, and tylosin). The study was designed with a semi-field approach in micro-hives containing about 500 honeybees. Micro-hives were located in an incubator during the day and moved outdoors in the late afternoon, considering the restrictions on the use of antibiotics in the open field but allowing a certain freedom to honeybees; 6 replicates were considered for each treatment. The absolute abundance of the major gut microbial taxa in newly eclosed individuals was studied with qPCR and next-generation sequencing. Antimicrobial resistance genes for the target antibiotics were also monitored using a qPCR approach. The results showed that the total amount of gut bacteria was not altered by antibiotic treatment, but qualitative variations were observed. Tylosin treatment determined a significant decrease of α- and β-diversity indices and a strong depletion of the rectum population (lactobacilli and bifidobacteria) while favoring the ileum microorganisms (
Identifiants
pubmed: 34378962
doi: 10.1128/Spectrum.00176-21
pmc: PMC8552759
doi:
Substances chimiques
Anti-Bacterial Agents
0
Sulfonamides
0
Veterinary Drugs
0
Oxytetracycline
X20I9EN955
Tylosin
YEF4JXN031
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0017621Références
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