Circulating Mir-140 and leptin improve the accuracy of the differential diagnosis between psoriatic arthritis and rheumatoid arthritis: a case-control study.
ACPA = anti‐cyclic citrullinated peptide antibodies
ACR = American college of rheumatology
ACTB = actin beta
BMI = body mass index
CRP = C-reactive protein
CV = cardiovascular
DAPSA = disease activity in psoriatic arthritis
DAS28 = disease activity score 28
DMARDs = disease-modifying anti-rheumatic drugs
ESR = erythrocyte sedimentation rate
EULAR = European league against rheumatism
HAQ = health assessment questionnaire
HC = healthy controls
HDL = high density lipoprotein
HMW = high molecular weight
IGF = insulin-like growth factor
IL = interleukin
LDL = low density lipoprotein
LMW = low molecular weight
MAPK = mitogen-activated protein kinase
MMW = medium molecular weight
NF‐κB = nuclear factor κB
NSAIDs = non-steroidal anti-inflammatory drugs
OA = osteoarthritis
PASI = psoriasis area severity index
PBMCs = peripheral blood mononuclear cells
PsA = psoriatic arthritis
RA = rheumatoid arthritis
RF = rheumatoid factor
SIRT‐1 = silencing the gene for sirtuin
SNORD-25 = small nucleolar RNA = C/D Box 25
TGF-β = activity and in transforming growth factor
TNF = tumor necrosis factor
VAS = visual analogue scale
VEGF = vascular endothelial growth factor
miRNA = microRNA
Journal
Translational research : the journal of laboratory and clinical medicine
ISSN: 1878-1810
Titre abrégé: Transl Res
Pays: United States
ID NLM: 101280339
Informations de publication
Date de publication:
01 2022
01 2022
Historique:
received:
23
02
2021
revised:
03
08
2021
accepted:
03
08
2021
pubmed:
12
8
2021
medline:
4
1
2022
entrez:
11
8
2021
Statut:
ppublish
Résumé
The differential diagnosis of psoriatic arthritis (PsA) and rheumatoid arthritis (RA) is difficult because of the lack of diagnostic clinical signs and reliable biomarkers. This study investigated microRNAs (miRNA) and adipokines as potential additional markers to discriminate PsA from RA. The expression profile of miRNA (miR-21, miR-140, miR-146a, miR-155, miR-181b, miR-223, miR-let-7e) and inflammatory cytokines (IL-1β, IL-6, IL-17a, IL-23a, TNF-α) from peripheral blood mononuclear cells of PsA and RA patients compared to healthy controls (HC) were evaluated by real-time PCR, and serum adipokines (adiponectin, chemerin, leptin, resistin, visfatin) and cytokines by ELISA assay. Univariable binary logistic regression was used to find the association between PsA and potential predictors. The gene expression of miRNA and cytokines and the serum levels of adipokines were found significantly different in PsA and RA patients compared to HC, as well as in PsA versus RA. MiR-140 gene expression resulted up-regulated in PsA patients and reduced in RA in comparison to HC, and, for the first time, significantly higher in PsA compared with RA. Serum levels of IL-23a and leptin were significantly increased in PsA and RA populations than in HC, as well as in PsA versus RA. Furthermore, circulating TNF-α was up-regulated in PsA and RA in comparison to controls, while resulted higher in RA than in PsA. Univariable binary logistic regression analysis found the above-mentioned markers associated to PsA versus RA. Our results first demonstrated an increased expression of circulating miR-140 and serum leptin in PsA patients compared to RA, which were identified as potential additional biomarkers to discriminate PsA from RA. Since the differential diagnosis of PsA and RA poses challenges in clinical practice, our data may help to enhance the diagnostic performance of PsA in daily practice.
Identifiants
pubmed: 34380068
pii: S1931-5244(21)00191-2
doi: 10.1016/j.trsl.2021.08.001
pii:
doi:
Substances chimiques
Adipokines
0
Biomarkers
0
Cytokines
0
LEP protein, human
0
Leptin
0
MicroRNAs
0
Mirn140 microRNA, human
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
18-34Informations de copyright
Copyright © 2021 Elsevier Inc. All rights reserved.