Investigating the two regimes of fibrin clot lysis: an experimental and computational approach.
Journal
Biophysical journal
ISSN: 1542-0086
Titre abrégé: Biophys J
Pays: United States
ID NLM: 0370626
Informations de publication
Date de publication:
21 09 2021
21 09 2021
Historique:
received:
05
05
2021
revised:
07
07
2021
accepted:
04
08
2021
pubmed:
14
8
2021
medline:
29
10
2021
entrez:
13
8
2021
Statut:
ppublish
Résumé
It has been observed in vitro that complete clot lysis is generally preceded by a slow phase of lysis during which the degradation seems to be inefficient. However, this slow regime was merely noticed, but not yet quantitatively discussed. In our experiments, we observed that the lysis ubiquitously occurred in two distinct regimes, a slow and a fast lysis regime. We quantified extensively the duration of these regimes for a wide spectrum of experimental conditions and found that on average, the slow regime lasts longer than the fast one, meaning that during most of the process, the lysis is ineffective. We proposed a computational model in which the properties of the binding of the proteins change during the lysis: first, the biochemical reactions take place at the surface of the fibrin fibers, then in the bulk, resulting in the observed fast lysis regime. This simple hypothesis appeared to be sufficient to reproduce with a great accuracy the lysis profiles obtained experimentally.
Identifiants
pubmed: 34384765
pii: S0006-3495(21)00650-0
doi: 10.1016/j.bpj.2021.08.005
pmc: PMC8510862
pii:
doi:
Substances chimiques
Fibrin
9001-31-4
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
4091-4106Informations de copyright
Copyright © 2021 Biophysical Society. Published by Elsevier Inc. All rights reserved.
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