Development of a functional impedimetric immunosensor for accurate detection of thyroid-stimulating hormone.
4-mercaptophenylacetic acid
immunosensor
impedance spectroscopy
self-assembled monolayers
Thyroid-stimulating hormone
Journal
Turkish journal of chemistry
ISSN: 1300-0527
Titre abrégé: Turk J Chem
Pays: Turkey
ID NLM: 101259104
Informations de publication
Date de publication:
2021
2021
Historique:
received:
30
12
2020
accepted:
26
03
2021
entrez:
13
8
2021
pubmed:
14
8
2021
medline:
14
8
2021
Statut:
epublish
Résumé
Thyroid-stimulating hormone (TSH), which regulates the synthesis of thyroid gland hormones affecting the whole metabolism, is a pituitary hormone. Determination of TSH is crucial for monitoring thyroid gland-related disorders and some metabolic diseases.In this study, a nonlabeled immunosensor based on covalent immobilization of anti-TSH antibody by using the formation of self-assembled monolayers (SAM) of 4-mercaptophenylacetic acid (4-MPA) and functionalization of carboxyl ends with 1-ethyl-3-(3-dimetilaminopropil) carbodiimide (EDC)/N-Hydroxysuccinimide (NHS) was fabricated for detection of TSH. Immobilization steps including the concentration of 4-MPA, the concentration of anti-TSH antibody, and duration of anti-TSH antibody incubation were optimized by utilizing electrochemical impedance spectroscopy. Under optimal conditions, a sensitive, rapid, and accurate determination of TSH at a concentration range between 0.7 and 3.5 mIU/L was accomplished with a notable linearity and LOD value of 0.034 mIU/L, as well as reproducibility and repeatability. Moreover, for comparison, linear range experiments were also carried out by using other electrochemical methods, including linear sweep voltammetry, cyclic voltammetry, and capacitance spectroscopy. Finally, the constructed immunosensor was used for analyzing TSH levels spiked in the artificial serum samples.
Identifiants
pubmed: 34385869
doi: 10.3906/kim-2012-69
pmc: PMC8329345
doi:
Types de publication
Journal Article
Langues
eng
Pagination
819-834Informations de copyright
Copyright © 2021 The Author(s).
Déclaration de conflit d'intérêts
CONFLICT OF INTEREST: none declared
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