The epithelial potassium channel Kir7.1 is stimulated by progesterone.
Journal
The Journal of general physiology
ISSN: 1540-7748
Titre abrégé: J Gen Physiol
Pays: United States
ID NLM: 2985110R
Informations de publication
Date de publication:
04 10 2021
04 10 2021
Historique:
received:
02
04
2021
accepted:
29
07
2021
entrez:
13
8
2021
pubmed:
14
8
2021
medline:
18
3
2022
Statut:
ppublish
Résumé
The choroid plexus (CP) epithelium secretes cerebrospinal fluid and plays an important role in healthy homeostasis of the brain. CP function can be influenced by sex steroid hormones; however, the precise molecular mechanism of such regulation is not well understood. Here, using whole-cell patch-clamp recordings from male and female murine CP cells, we show that application of progesterone resulted in specific and strong potentiation of the inwardly rectifying potassium channel Kir7.1, an essential protein that is expressed in CP and is required for survival. The potentiation was progesterone specific and independent of other known progesterone receptors expressed in CP. This effect was recapitulated with recombinant Kir7.1, as well as with endogenous Kir7.1 expressed in the retinal pigment epithelium. Current-clamp studies further showed a progesterone-induced hyperpolarization of CP cells. Our results provide evidence of a progesterone-driven control of tissues in which Kir7.1 is present.
Identifiants
pubmed: 34387656
pii: 212552
doi: 10.1085/jgp.202112924
pmc: PMC8374857
pii:
doi:
Substances chimiques
Potassium Channels, Inwardly Rectifying
0
Progesterone
4G7DS2Q64Y
Potassium
RWP5GA015D
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : NIGMS NIH HHS
ID : R01 GM111802
Pays : United States
Organisme : NIA NIH HHS
ID : R03 AG070755
Pays : United States
Organisme : NIH HHS
ID : S10 OD018174
Pays : United States
Organisme : NIH HHS
ID : R01GM111802
Pays : United States
Informations de copyright
© 2021 Björkgren et al.
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