A Sinorhizobium meliloti and Agrobacterium tumefaciens ExoR ortholog is not crucial for Brucella abortus virulence.
Agrobacterium tumefaciens
/ drug effects
Animals
Bacterial Proteins
/ genetics
Brucella abortus
/ genetics
Brucellosis
/ genetics
Gene Expression Regulation, Bacterial
/ genetics
Host-Parasite Interactions
/ genetics
Humans
Mice
Mutation
/ genetics
Polymyxin B
/ pharmacology
Sinorhizobium meliloti
/ drug effects
Virulence
/ genetics
Journal
PloS one
ISSN: 1932-6203
Titre abrégé: PLoS One
Pays: United States
ID NLM: 101285081
Informations de publication
Date de publication:
2021
2021
Historique:
received:
03
02
2021
accepted:
29
06
2021
entrez:
13
8
2021
pubmed:
14
8
2021
medline:
25
11
2021
Statut:
epublish
Résumé
Brucella is a facultative extracellular-intracellular pathogen that belongs to the Alphaproteobacteria class. Precise sensing of environmental changes and a proper response mediated by a gene expression regulatory network are essential for this pathogen to survive. The plant-related Alphaproteobacteria Sinorhizobium meliloti and Agrobacterium tumefaciens also alternate from a free to a host-associated life, where a regulatory invasion switch is needed for this transition. This switch is composed of a two-component regulatory system (TCS) and a global inhibitor, ExoR. In B. abortus, the BvrR/BvrS TCS is essential for intracellular survival. However, the presence of a TCS inhibitor, such as ExoR, in Brucella is still unknown. In this work, we identified a genomic sequence similar to S. meliloti exoR in the B. abortus 2308W genome, constructed an exoR mutant strain, and performed its characterization through ex vivo and in vivo assays. Our findings indicate that ExoR is related to the BvrR phosphorylation state, and is related to the expression of known BvrR/BrvS gene targets, such as virB8, vjbR, and omp25 when grown in rich medium or starving conditions. Despite this, the exoR mutant strain showed no significant differences as compared to the wild-type strain, related to resistance to polymyxin B or human non-immune serum, intracellular replication, or infectivity in a mice model. ExoR in B. abortus is related to BvrR/BvrS as observed in other Rhizobiales; however, its function seems different from that observed for its orthologs described in A. tumefaciens and S. meliloti.
Identifiants
pubmed: 34388167
doi: 10.1371/journal.pone.0254568
pii: PONE-D-21-03810
pmc: PMC8362948
doi:
Substances chimiques
Bacterial Proteins
0
Polymyxin B
J2VZ07J96K
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0254568Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
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