Streptococcal Exotoxin Streptolysin O Causes Vascular Endothelial Dysfunction Through PKC

Animals Aorta, Thoracic / drug effects Bacterial Proteins / toxicity Dose-Response Relationship, Drug Endothelium, Vascular / drug effects Male Mesenteric Arteries / drug effects Organ Culture Techniques Protein Kinase C beta / metabolism Rats Rats, Inbred SHR Rats, Inbred WKY Rats, Wistar Streptolysins / toxicity Vasoconstriction / drug effects

Journal

The Journal of pharmacology and experimental therapeutics
ISSN: 1521-0103
Titre abrégé: J Pharmacol Exp Ther
Pays: United States
ID NLM: 0376362

Informations de publication

Date de publication:
11 2021
Historique:
received: 23 05 2021
accepted: 10 08 2021
pubmed: 15 8 2021
medline: 30 12 2021
entrez: 14 8 2021
Statut: ppublish

Résumé

Streptolysin O (SLO) is produced by common hemolytic streptococci that cause a wide range of diseases from pharyngitis to life-threatening necrotizing fasciitis and toxic shock syndrome. Although the importance of SLO in invasive hemolytic streptococcus infection has been well demonstrated, the role of circulating SLO in noninvasive infection remains unclear. The aim of this study was to characterize the pharmacological effect of SLO on vascular functions, focusing on cellular signaling pathways. In control Wistar rats, SLO treatment (1-1000 ng/ml) impaired acetylcholine-induced endothelial-dependent relaxation in the aorta and second-order mesenteric artery in a dose-dependent manner without any effects on sodium nitroprusside-induced endothelium-independent relaxation or agonist-induced contractions. SLO also increased phosphorylation of the endothelial NO synthase (eNOS) inhibitory site at Thr495 in the aorta. Pharmacological analysis indicated that either endothelial dysfunction or eNOS phosphorylation was mediated by protein kinase C

Identifiants

DOI: 10.1124/jpet.121.000752 PMID: 34389653
pubmed: 34389653
pii: jpet.121.000752
doi: 10.1124/jpet.121.000752
doi:

Substances chimiques

Bacterial Proteins 0
Streptolysins 0
streptolysin O 0
Protein Kinase C beta EC 2.7.11.13

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

117-124

Informations de copyright

Copyright © 2021 by The American Society for Pharmacology and Experimental Therapeutics.

Auteurs

Masashi Mukohda (M)

Laboratory of Veterinary Pharmacology (M.M., M.S., R.M., H.O.), Animal Health (S.N.), Biochemistry (K.T.), Veterinary Internal Medicine, Faculty of Veterinary Medicine (A.K.), and Laboratory of Microbiology, Faculty of Science, Okayama University of Science, Japan (T.K.) m-mukohda@vet.ous.ac.jp.

Sho Nakamura (S)

Laboratory of Veterinary Pharmacology (M.M., M.S., R.M., H.O.), Animal Health (S.N.), Biochemistry (K.T.), Veterinary Internal Medicine, Faculty of Veterinary Medicine (A.K.), and Laboratory of Microbiology, Faculty of Science, Okayama University of Science, Japan (T.K.).

Kosuke Takeya (K)

Laboratory of Veterinary Pharmacology (M.M., M.S., R.M., H.O.), Animal Health (S.N.), Biochemistry (K.T.), Veterinary Internal Medicine, Faculty of Veterinary Medicine (A.K.), and Laboratory of Microbiology, Faculty of Science, Okayama University of Science, Japan (T.K.).

Akira Matsuda (A)

Laboratory of Veterinary Pharmacology (M.M., M.S., R.M., H.O.), Animal Health (S.N.), Biochemistry (K.T.), Veterinary Internal Medicine, Faculty of Veterinary Medicine (A.K.), and Laboratory of Microbiology, Faculty of Science, Okayama University of Science, Japan (T.K.).

Takanori Yano (T)

Laboratory of Veterinary Pharmacology (M.M., M.S., R.M., H.O.), Animal Health (S.N.), Biochemistry (K.T.), Veterinary Internal Medicine, Faculty of Veterinary Medicine (A.K.), and Laboratory of Microbiology, Faculty of Science, Okayama University of Science, Japan (T.K.).

Mihiro Seki (M)

Laboratory of Veterinary Pharmacology (M.M., M.S., R.M., H.O.), Animal Health (S.N.), Biochemistry (K.T.), Veterinary Internal Medicine, Faculty of Veterinary Medicine (A.K.), and Laboratory of Microbiology, Faculty of Science, Okayama University of Science, Japan (T.K.).

Risuke Mizuno (R)

Laboratory of Veterinary Pharmacology (M.M., M.S., R.M., H.O.), Animal Health (S.N.), Biochemistry (K.T.), Veterinary Internal Medicine, Faculty of Veterinary Medicine (A.K.), and Laboratory of Microbiology, Faculty of Science, Okayama University of Science, Japan (T.K.).

Hiroshi Ozaki (H)

Laboratory of Veterinary Pharmacology (M.M., M.S., R.M., H.O.), Animal Health (S.N.), Biochemistry (K.T.), Veterinary Internal Medicine, Faculty of Veterinary Medicine (A.K.), and Laboratory of Microbiology, Faculty of Science, Okayama University of Science, Japan (T.K.).

Articles similaires

Smoking Cessation and Incident Cardiovascular Disease.

Jun Hwan Cho, Seung Yong Shin, Hoseob Kim et al.
1.00
Humans Male Smoking Cessation Cardiovascular Diseases Female

Evaluation of Low-Value Services Across Major Medicare Advantage Insurers and Traditional Medicare.

Ciara Duggan, Adam L Beckman, Ishani Ganguli et al.
1.00
Humans United States Aged Cross-Sectional Studies Medicare Part C

Effectiveness of Virtual Yoga for Chronic Low Back Pain: A Randomized Clinical Trial.

Hallie Tankha, Devyn Gaskins, Amanda Shallcross et al.
1.00
Humans Yoga Low Back Pain Female Male

Meal Timing and Anthropometric and Metabolic Outcomes: A Systematic Review and Meta-Analysis.

Hiu Yee Liu, Ashley A Eso, Nathan Cook et al.
1.00
Humans Meals Time Factors Female Adult

Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Streptococcal Exotoxin Streptolysin O Causes...
questionsmedicales.fr › Système cardiovasculaire › Vaisseaux sanguins › Artères › Aorte › Aorte thoracique › Streptococcal Exotoxin Streptolysin O Causes...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines bactériennes › Streptococcal Exotoxin Streptolysin O Causes...
questionsmedicales.fr › Phénomènes physiologiques › Phénomènes pharmacologiques et toxicologiques › Phénomènes toxicologiques › Relation dose-effet des médicaments › Streptococcal Exotoxin Streptolysin O Causes...
questionsmedicales.fr › Tissus › Épithélium › Endothélium › Endothélium vasculaire › Streptococcal Exotoxin Streptolysin O Causes...
questionsmedicales.fr › Système cardiovasculaire › Vaisseaux sanguins › Artères › Artères mésentériques › Streptococcal Exotoxin Streptolysin O Causes...
questionsmedicales.fr › Techniques d'investigation › Techniques in vitro › Techniques de culture › Techniques de culture d'organes › Streptococcal Exotoxin Streptolysin O Causes...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines et peptides de signalisation intracellulaire › Protein-Serine-Threonine Kinases › Protéine kinase C › Protein kinase C beta › Streptococcal Exotoxin Streptolysin O Causes...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Rats › Streptococcal Exotoxin Streptolysin O Causes...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Rats › Lignées consanguines de rats › Rats de lignée SHR › Streptococcal Exotoxin Streptolysin O Causes...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Rats › Lignées consanguines de rats › Rats de lignée WKY › Streptococcal Exotoxin Streptolysin O Causes...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Rats › Rat Wistar › Streptococcal Exotoxin Streptolysin O Causes...
questionsmedicales.fr › Facteurs biologiques › Toxines biologiques › Exotoxines › Streptolysines › Streptococcal Exotoxin Streptolysin O Causes...
questionsmedicales.fr › Phénomènes physiologiques respiratoires et circulatoires › Phénomènes physiologiques cardiovasculaires › Hémodynamique › Vasoconstriction › Streptococcal Exotoxin Streptolysin O Causes...