The missing linker between SUN5 and PMFBP1 in sperm head-tail coupling apparatus.
Animals
Cell Cycle Proteins
Cells, Cultured
Cytoskeletal Proteins
/ genetics
HEK293 Cells
Humans
Infertility, Male
/ genetics
Male
Membrane Proteins
/ genetics
Mice, Inbred C57BL
Mice, Inbred DBA
Mice, Knockout
Mutation
Protein Binding
Sperm Head
/ metabolism
Sperm Tail
/ metabolism
Spermatozoa
/ cytology
Teratozoospermia
/ genetics
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
13 08 2021
13 08 2021
Historique:
received:
23
03
2020
accepted:
27
07
2021
entrez:
14
8
2021
pubmed:
15
8
2021
medline:
31
8
2021
Statut:
epublish
Résumé
The sperm head-to-tail coupling apparatus (HTCA) ensures sperm head-tail integrity while defective HTCA causes acephalic spermatozoa, rendering males infertile. Here, we show that CENTLEIN is indispensable for HTCA integrity and function, and that inactivation of CENTLEIN in mice leads to sperm decapitation and male sterility. We demonstrate that CENTLEIN directly interacts with both SUN5 and PMFBP1, two proteins localized in the HTCA and related with acephalic spermatozoa syndrome. We find that the absence of Centlein sets SUN5 and PMFBP1 apart, the former close to the sperm head and the latter in the decapitated tail. We show that lack of Sun5 results in CENTLEIN and PMFBP1 left in the decapitated tail, while disruption of Pmfbp1 results in SUN5 and CENTLEIN left on the detached sperm head. These results demonstrate that CENTLEIN cooperating with SUN5 and PMFBP1 participates in the HTCA assembly and integration of sperm head to the tail, indicating that impairments of CENTLEIN might be associated with acephalic spermatozoa syndrome in humans.
Identifiants
pubmed: 34389728
doi: 10.1038/s41467-021-25227-w
pii: 10.1038/s41467-021-25227-w
pmc: PMC8363609
doi:
Substances chimiques
Cell Cycle Proteins
0
Cytoskeletal Proteins
0
Membrane Proteins
0
Pmfbp1 protein, mouse
0
SUN5 protein, mouse
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
4926Informations de copyright
© 2021. The Author(s).
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