Multiple and early hyperbaric oxygen treatments enhance muscle healing after muscle contusion injury: a pilot study.
Animals
Cell Differentiation
Cell Proliferation
/ physiology
Contusions
/ physiopathology
Hyperbaric Oxygenation
/ methods
Macrophages
/ physiology
Male
Muscle Contraction
/ physiology
Muscle Strength
/ physiology
Muscle, Skeletal
/ injuries
Pilot Projects
Random Allocation
Rats
Rats, Wistar
Satellite Cells, Skeletal Muscle
/ physiology
Time-to-Treatment
Wound Healing
/ physiology
contusion
macrophages
muscle recovery
muscle regeneration
satellite cells
Journal
Undersea & hyperbaric medicine : journal of the Undersea and Hyperbaric Medical Society, Inc
ISSN: 1066-2936
Titre abrégé: Undersea Hyperb Med
Pays: United States
ID NLM: 9312954
Informations de publication
Date de publication:
Historique:
entrez:
14
8
2021
pubmed:
15
8
2021
medline:
2
9
2021
Statut:
ppublish
Résumé
The optimal timing of hyperbaric oxygen (HBO2) treatments for the best recovery following muscle injury has yet to be determined. Thus, the optimal number and timing of HBO2 treatments for maximal muscle regeneration were explored. The HBO2 treatment protocol consisted of 2.5 ATA 100% oxygen for 120 minutes. Muscle-injured rats were randomized to one of 10 groups: single HBO2 treatment immediately after injury (HBO 1T day 0), one day (HBO 1T day 1), three days (HBO 1T day 3) and five days (HBO 1T day 5) after injury; three HBO2 treatments from immediately after injury to two days after injury (HBO 3T day 0-2), from one to three days after injury (HBO 3T day 1-3), from three to five days after injury (HBO 3T day 3-5), from five to seven days after injury (HBO 3T day 5-7); five daily HBO2 treatments (HBO 5T); and no treatment (NT). HBO 5T and HBO 3T day 0-2, days 1-3 and days 3-5 significantly promoted CD206-positive cell infiltration, satellite cell differentiation and muscle regeneration compared to the NT group. Five HBO2 treatments and three HBO2 treatments within three days of injury promote muscle regeneration.
Sections du résumé
BACKGROUND
BACKGROUND
The optimal timing of hyperbaric oxygen (HBO2) treatments for the best recovery following muscle injury has yet to be determined. Thus, the optimal number and timing of HBO2 treatments for maximal muscle regeneration were explored.
METHODS
METHODS
The HBO2 treatment protocol consisted of 2.5 ATA 100% oxygen for 120 minutes. Muscle-injured rats were randomized to one of 10 groups: single HBO2 treatment immediately after injury (HBO 1T day 0), one day (HBO 1T day 1), three days (HBO 1T day 3) and five days (HBO 1T day 5) after injury; three HBO2 treatments from immediately after injury to two days after injury (HBO 3T day 0-2), from one to three days after injury (HBO 3T day 1-3), from three to five days after injury (HBO 3T day 3-5), from five to seven days after injury (HBO 3T day 5-7); five daily HBO2 treatments (HBO 5T); and no treatment (NT).
RESULTS
RESULTS
HBO 5T and HBO 3T day 0-2, days 1-3 and days 3-5 significantly promoted CD206-positive cell infiltration, satellite cell differentiation and muscle regeneration compared to the NT group.
CONCLUSION
CONCLUSIONS
Five HBO2 treatments and three HBO2 treatments within three days of injury promote muscle regeneration.
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
227-238Informations de copyright
Copyright© Undersea and Hyperbaric Medical Society.
Déclaration de conflit d'intérêts
The authors of this paper declare no conflicts of interest exist with this submission.