Comparison of Freelite and N-Latex serum free light chain assays: a critical review.
analytical techniques and equipment
comparison
free light chain
immunoassays
monoclonal gammopathies
Journal
Biochemia medica
ISSN: 1846-7482
Titre abrégé: Biochem Med (Zagreb)
Pays: Croatia
ID NLM: 9610305
Informations de publication
Date de publication:
15 Oct 2021
15 Oct 2021
Historique:
received:
08
02
2021
accepted:
12
06
2021
entrez:
16
8
2021
pubmed:
17
8
2021
medline:
28
9
2021
Statut:
ppublish
Résumé
The measurement of serum free light chain (FLC) represents a fundamental aspect on the assessment of patients with monoclonal gammopathies (MG). Different analytical methods for FLC have become available with the possibility to obtain different value with a substantial impact on the assessment of patients with MG. This study aimed to evaluate FLC results obtained with two different assays and how the difference value obtained can impact in the patient's assessment. Ninety-three patient serum samples that underwent analysis for FLC with two different methods, Serum Freelite (The Binding Site, Birmingham, UK) and N-Latex FLC (Siemens, Marburg, Germany), were included in this retrospective study. Statistical analysis was performed to evaluate correlation, difference, and the grade of concordance between the results obtained with the two methods. Significant statistical differences between the results obtained from the two methods were found (P < 0.05). A good correlation was found (0.99 for κ FLC, 0.95 for λ FLC, and 0.94 for the κ/λ ratio, respectively). We found a weighted kappa value of 0.65 for κ/λ ratio, 0.65 for λ FLC and 0.90 for κ FLC. A positive bias found with the Bland-Altman plot mirrors overestimation of κ FLC and κ/λ ratio with Freelite compared to N-Latex, whilst a negative bias underscores underestimation of λ FLC by Freelite compared to N-Latex. Although in general the concordance between Freelite and N-Latex appears satisfactory, several discrepancies could be evidenced and consequently the two assays are not interchangeable.
Identifiants
pubmed: 34393594
doi: 10.11613/BM.2021.030701
pii: bm-31-3-030701
pmc: PMC8340501
doi:
Substances chimiques
Immunoglobulin Light Chains
0
Latex
0
Types de publication
Comparative Study
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
030701Informations de copyright
Croatian Society of Medical Biochemistry and Laboratory Medicine.
Déclaration de conflit d'intérêts
Potential conflict of interest None declared.
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