Transthyretin as a target of alkylation and a potential biomarker for sulfur mustard poisoning: Electrophoretic and mass spectrometric identification and characterization.
2D-thiol-DIGE
high-resolution mass spectrometry
sulfur mustard
transthyretin
verification
Journal
Drug testing and analysis
ISSN: 1942-7611
Titre abrégé: Drug Test Anal
Pays: England
ID NLM: 101483449
Informations de publication
Date de publication:
Jan 2022
Jan 2022
Historique:
revised:
06
08
2021
received:
07
05
2021
accepted:
09
08
2021
pubmed:
17
8
2021
medline:
24
3
2022
entrez:
16
8
2021
Statut:
ppublish
Résumé
For the verification of exposure to the banned blister agent sulfur mustard (SM) and the better understanding of its pathophysiology, protein adducts formed with endogenous proteins represent an important field of toxicological research. SM and its analogue 2-chloroethyl ethyl sulfide (CEES) are well known to alkylate nucleophilic amino acid side chains, for example, free-thiol groups of cysteine residues. The specific two-dimensional thiol difference gel electrophoresis (2D-thiol-DIGE) technique making use of maleimide dyes allows the staining of free cysteine residues in proteins. As a consequence of alkylation by, for example, SM or CEES, this staining intensity is reduced. 2D-thiol-DIGE analysis of human plasma incubated with CEES and subsequent matrix-assisted laser desorption/ionization time-of-flight (tandem) mass-spectrometry, MALDI-TOF MS(/MS), revealed transthyretin (TTR) as a target of alkylating agents. TTR was extracted from SM-treated plasma by immunomagnetic separation (IMS) and analyzed after tryptic cleavage by microbore liquid chromatography-electrospray ionization high-resolution tandem-mass spectrometry (μLC-ESI MS/HR MS). It was found that the Cys
Substances chimiques
Biomarkers
0
Chemical Warfare Agents
0
Prealbumin
0
TTR protein, human
0
2-chloroethyl ethyl sulfide
693-07-2
Mustard Gas
T8KEC9FH9P
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
80-91Subventions
Organisme : German Research Foundation
ID : GRK 2338
Informations de copyright
© 2021 The Authors. Drug Testing and Analysis published by John Wiley & Sons Ltd.
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