Electrophysiological engineering of heart-derived cells with calcium-dependent potassium channels improves cell therapy efficacy for cardioprotection.


Journal

Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555

Informations de publication

Date de publication:
16 08 2021
Historique:
received: 03 07 2019
accepted: 21 07 2021
entrez: 17 8 2021
pubmed: 18 8 2021
medline: 31 8 2021
Statut: epublish

Résumé

We have shown that calcium-activated potassium (KCa)-channels regulate fundamental progenitor-cell functions, including proliferation, but their contribution to cell-therapy effectiveness is unknown. Here, we test the participation of KCa-channels in human heart explant-derived cell (EDC) physiology and therapeutic potential. TRAM34-sensitive KCa3.1-channels, encoded by the KCNN4 gene, are exclusively expressed in therapeutically bioactive EDC subfractions and maintain a strongly polarized resting potential; whereas therapeutically inert EDCs lack KCa3.1 channels and exhibit depolarized resting potentials. Somatic gene transfer of KCNN4 results in membrane hyperpolarization and increases intracellular [Ca

Identifiants

pubmed: 34400625
doi: 10.1038/s41467-021-25180-8
pii: 10.1038/s41467-021-25180-8
pmc: PMC8368210
doi:

Substances chimiques

Cytokines 0
Intermediate-Conductance Calcium-Activated Potassium Channels 0
KCNN4 protein, human 0
Kcnn4 protein, mouse 0
Potassium Channel Blockers 0
Potassium Channels, Calcium-Activated 0
Potassium RWP5GA015D
Calcium SY7Q814VUP

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

4963

Subventions

Organisme : CIHR
ID : 148401
Pays : Canada
Organisme : CIHR
ID : 162088
Pays : Canada

Informations de copyright

© 2021. The Author(s).

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Auteurs

Patrick Vigneault (P)

Research Center and Department of Medicine, Montreal Heart Institute, Université de Montréal, Montreal, QC, Canada.

Sandrine Parent (S)

Division of Cardiology, Department of Medicine, University of Ottawa, Ottawa, ON, Canada.
Department of Cellular and Molecular Medicine, Faculty of Medicine, University of Ottawa, Ottawa, ON, Canada.

Pushpinder Kanda (P)

Division of Cardiology, Department of Medicine, University of Ottawa, Ottawa, ON, Canada.
Department of Cellular and Molecular Medicine, Faculty of Medicine, University of Ottawa, Ottawa, ON, Canada.

Connor Michie (C)

Division of Cardiology, Department of Medicine, University of Ottawa, Ottawa, ON, Canada.
Department of Cellular and Molecular Medicine, Faculty of Medicine, University of Ottawa, Ottawa, ON, Canada.

Darryl R Davis (DR)

Division of Cardiology, Department of Medicine, University of Ottawa, Ottawa, ON, Canada. ddavis@ottawaheart.ca.
Department of Cellular and Molecular Medicine, Faculty of Medicine, University of Ottawa, Ottawa, ON, Canada. ddavis@ottawaheart.ca.

Stanley Nattel (S)

Research Center and Department of Medicine, Montreal Heart Institute, Université de Montréal, Montreal, QC, Canada. stanley.nattel@icm-mhi.org.
Department of Pharmacology and Therapeutics, McGill University, Montreal, QC, Canada. stanley.nattel@icm-mhi.org.
Institute of Pharmacology, West German Heart and Vascular Center, Faculty of Medicine, University Duisburg-Essen, Essen, Germany. stanley.nattel@icm-mhi.org.
IHU LIRYC and Fondation Bordeaux Université, Bordeaux, France. stanley.nattel@icm-mhi.org.

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