Exploring rare cellular activity in more than one million cells by a transscale scope.

Animals Brain / cytology Calcium / analysis Cells / cytology Cyclic AMP / analysis Dictyostelium / chemistry Dogs Entosis Epithelial Cells / ultrastructure Equipment Design Green Fluorescent Proteins HeLa Cells / chemistry Humans Interneurons / ultrastructure Luminescent Proteins Madin Darby Canine Kidney Cells Mice Microscopy, Fluorescence / instrumentation Neurons / ultrastructure Semiconductors Red Fluorescent Protein

Journal

Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288

Informations de publication

Date de publication:
16 08 2021
Historique:
received: 12 05 2021
accepted: 03 08 2021
entrez: 17 8 2021
pubmed: 18 8 2021
medline: 16 11 2021
Statut: epublish

Résumé

In many phenomena of biological systems, not a majority, but a minority of cells act on the entire multicellular system causing drastic changes in the system properties. To understand the mechanisms underlying such phenomena, it is essential to observe the spatiotemporal dynamics of a huge population of cells at sub-cellular resolution, which is difficult with conventional tools such as microscopy and flow cytometry. Here, we describe an imaging system named AMATERAS that enables optical imaging with an over-one-centimeter field-of-view and a-few-micrometer spatial resolution. This trans-scale-scope has a simple configuration, composed of a low-power lens for machine vision and a hundred-megapixel image sensor. We demonstrated its high cell-throughput, capable of simultaneously observing more than one million cells. We applied it to dynamic imaging of calcium ions in HeLa cells and cyclic-adenosine-monophosphate in Dictyostelium discoideum, and successfully detected less than 0.01% of rare cells and observed multicellular events induced by these cells.

Identifiants

DOI: 10.1038/s41598-021-95930-7 PMID: 34400683 PMC: PMC8368064
pubmed: 34400683
doi: 10.1038/s41598-021-95930-7
pii: 10.1038/s41598-021-95930-7
pmc: PMC8368064
doi:

Substances chimiques

Luminescent Proteins 0
enhanced green fluorescent protein 0
Green Fluorescent Proteins 147336-22-9
Cyclic AMP E0399OZS9N
Calcium SY7Q814VUP

Types de publication

Journal Article Research Support, Non-U.S. Gov't Video-Audio Media

Langues

eng

Sous-ensembles de citation

IM

Pagination

16539

Informations de copyright

© 2021. The Author(s).

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Auteurs

T Ichimura (T)

Transdimensional Life Imaging Division, Institute for Open and Transdisciplinary Research Initiatives, Osaka University, Yamadaoka 2-1, Suita, Osaka, 565-0871, Japan. ichimura@otri.osaka-u.ac.jp.
PRESTO, Japan Science and Technology Agency, Tokyo, 113-0033, Japan. ichimura@otri.osaka-u.ac.jp.

T Kakizuka (T)

Transdimensional Life Imaging Division, Institute for Open and Transdisciplinary Research Initiatives, Osaka University, Yamadaoka 2-1, Suita, Osaka, 565-0871, Japan.

K Horikawa (K)

Department of Optical Imaging, Advanced Research Promotion Center, Tokushima University, Kuramoto-cho 3-18-15, Tokushima, Tokushima, 770-8503, Japan.

K Seiriki (K)

Laboratory of Molecular Neuropharmacology, Graduate School of Pharmaceutical Sciences, Osaka University, Yamadaoka 1-6, Suita, Osaka, 565-0871, Japan.

A Kasai (A)

Laboratory of Molecular Neuropharmacology, Graduate School of Pharmaceutical Sciences, Osaka University, Yamadaoka 1-6, Suita, Osaka, 565-0871, Japan.

H Hashimoto (H)

Transdimensional Life Imaging Division, Institute for Open and Transdisciplinary Research Initiatives, Osaka University, Yamadaoka 2-1, Suita, Osaka, 565-0871, Japan.
Laboratory of Molecular Neuropharmacology, Graduate School of Pharmaceutical Sciences, Osaka University, Yamadaoka 1-6, Suita, Osaka, 565-0871, Japan.
Institute for Transdisciplinary Graduate Degree Programs, Osaka University, Yamadaoka 1-1, Suita, Osaka, 565-0871, Japan.

K Fujita (K)

Transdimensional Life Imaging Division, Institute for Open and Transdisciplinary Research Initiatives, Osaka University, Yamadaoka 2-1, Suita, Osaka, 565-0871, Japan.
Department of Applied Physics, Graduate School of Engineering, Osaka University, Yamadaoka 2-1, Suita, Osaka, 565-0871, Japan.

T M Watanabe (TM)

Laboratory for Comprehensive Bioimaging, RIKEN Center for Biosystems Dynamics Research (BDR), Minatomachi-minami 2-2-3, Chuo-ku, Kobe, Hyogo, 650-0047, Japan.
Department of Stem Cell Biology, Research Institute for Radiation Biology and Medicine, Hiroshima University, Kasumi 1-2-3, Minami-ku, Hiroshima, 734-8553, Japan.

T Nagai (T)

Transdimensional Life Imaging Division, Institute for Open and Transdisciplinary Research Initiatives, Osaka University, Yamadaoka 2-1, Suita, Osaka, 565-0871, Japan. ng1@sanken.osaka-u.ac.jp.
SANKEN (The Institute of Scientific and Industrial Research), Osaka University, Mihogaoka 8-1, Ibaraki, Osaka, 567-0047, Japan. ng1@sanken.osaka-u.ac.jp.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Exploring rare cellular activity in more than...
questionsmedicales.fr › Système nerveux › Système nerveux central › Encéphale › Exploring rare cellular activity in more than...
questionsmedicales.fr › Facteurs biologiques › Facteurs de la coagulation sanguine › Calcium › Exploring rare cellular activity in more than...
questionsmedicales.fr › Cellules › Exploring rare cellular activity in more than...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Nucléotides › Ribonucléotides › Nucléotides adényliques › AMP cyclique › Exploring rare cellular activity in more than...
questionsmedicales.fr › Eucaryotes › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium › Exploring rare cellular activity in more than...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Carnivora › Pinnipedia › Canidae › Chiens › Exploring rare cellular activity in more than...
questionsmedicales.fr › Phénomènes physiologiques cellulaires › Mécanismes conduisant à la présence d'une cellule dans une autre › Entose › Exploring rare cellular activity in more than...
questionsmedicales.fr › Cellules › Cellules épithéliales › Exploring rare cellular activity in more than...
questionsmedicales.fr › Techniques d'investigation › Conception d'appareillage › Exploring rare cellular activity in more than...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines luminescentes › Protéines à fluorescence verte › Exploring rare cellular activity in more than...
questionsmedicales.fr › Cellules › Cellules épithéliales › Cellules HeLa › Exploring rare cellular activity in more than...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Exploring rare cellular activity in more than...
questionsmedicales.fr › Cellules › Neurones › Interneurones › Exploring rare cellular activity in more than...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines luminescentes › Exploring rare cellular activity in more than...
questionsmedicales.fr › Cellules › Cellules épithéliales › Cellules rénales canines Madin-Darby › Exploring rare cellular activity in more than...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Souris › Exploring rare cellular activity in more than...
questionsmedicales.fr › Techniques d'investigation › Microscopie de fluorescence › Exploring rare cellular activity in more than...
questionsmedicales.fr › Cellules › Neurones › Exploring rare cellular activity in more than...
questionsmedicales.fr › Équipement et fournitures › Équipement et fournitures électriques › Semiconducteurs › Exploring rare cellular activity in more than...