Protocol for rapid manipulation of mitochondrial morphology in living cells using inducible counter mitochondrial morphology (iCMM).
Biotechnology and bioengineering
Cell Biology
Molecular/Chemical Probes
Protein Biochemistry
Journal
STAR protocols
ISSN: 2666-1667
Titre abrégé: STAR Protoc
Pays: United States
ID NLM: 101769501
Informations de publication
Date de publication:
17 09 2021
17 09 2021
Historique:
entrez:
17
8
2021
pubmed:
18
8
2021
medline:
18
8
2021
Statut:
epublish
Résumé
Disruption of mitochondrial morphology occurs during various diseases, but the biological significance is not entirely clear. Here, we describe a detailed step-by-step protocol for a chemically inducible dimerization system-based synthetic protein device, termed inducible counter mitochondrial morphology. This system allows artificial manipulation of mitochondrial morphology on a timescale of minutes in living mammalian cells. We also describe an AI-assisted imaging processing approach. For complete details on the use and execution of this protocol, please refer to Miyamoto et al., 2021.
Identifiants
pubmed: 34401787
doi: 10.1016/j.xpro.2021.100721
pii: S2666-1667(21)00428-7
pmc: PMC8353406
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Pagination
100721Informations de copyright
© 2021 The Author(s).
Déclaration de conflit d'intérêts
The authors declare no competing interests.
Références
Biochim Biophys Acta. 2015 Feb;1853(2):348-60
pubmed: 25447550
Pflugers Arch. 2013 Mar;465(3):409-17
pubmed: 23299847
Acta Histochem. 2017 Apr;119(3):315-326
pubmed: 28314612
Sci Rep. 2018 Nov 5;8(1):16354
pubmed: 30397207
Science. 2018 Mar 9;359(6380):
pubmed: 29590011