An in vitro stem cell model of human epiblast and yolk sac interaction.
cell biology
developmental biology
embryology
epiblast
human
human development
hypoblast
mouse
pluripotency
stem cells
Journal
eLife
ISSN: 2050-084X
Titre abrégé: Elife
Pays: England
ID NLM: 101579614
Informations de publication
Date de publication:
17 08 2021
17 08 2021
Historique:
received:
10
10
2020
accepted:
03
08
2021
entrez:
17
8
2021
pubmed:
18
8
2021
medline:
9
10
2021
Statut:
epublish
Résumé
Human embryogenesis entails complex signalling interactions between embryonic and extra-embryonic cells. However, how extra-embryonic cells direct morphogenesis within the human embryo remains largely unknown due to a lack of relevant stem cell models. Here, we have established conditions to differentiate human pluripotent stem cells (hPSCs) into yolk sac-like cells (YSLCs) that resemble the post-implantation human hypoblast molecularly and functionally. YSLCs induce the expression of pluripotency and anterior ectoderm markers in human embryonic stem cells (hESCs) at the expense of mesoderm and endoderm markers. This activity is mediated by the release of BMP and WNT signalling pathway inhibitors, and, therefore, resembles the functioning of the anterior visceral endoderm signalling centre of the mouse embryo, which establishes the anterior-posterior axis. Our results implicate the yolk sac in epiblast cell fate specification in the human embryo and propose YSLCs as a tool for studying post-implantation human embryo development
Identifiants
pubmed: 34403333
doi: 10.7554/eLife.63930
pii: 63930
pmc: PMC8370770
doi:
pii:
Banques de données
GEO
['GSE109555', 'GSE136447', 'GSE138012', 'GSE89303']
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Medical Research Council
ID : MC_PC_17230
Pays : United Kingdom
Organisme : NICHD NIH HHS
ID : DP1 HD104575
Pays : United States
Organisme : Wellcome Trust
ID : 207415/Z/17/Z
Pays : United Kingdom
Organisme : Medical Research Council
ID : MC_UP_1201/24
Pays : United Kingdom
Organisme : Wellcome Trust
Pays : United Kingdom
Organisme : Biotechnology and Biological Sciences Research Council
ID : 1943755
Pays : United Kingdom
Organisme : NICHD NIH HHS
ID : R01 HD100456
Pays : United States
Organisme : European Research Council
ID : 669198
Pays : International
Informations de copyright
© 2021, Mackinlay et al.
Déclaration de conflit d'intérêts
KM, BW, VS, CH, GH, TC, LP, SB, LV, MS, MZ No competing interests declared
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