A novel linear epitope at the C-terminal region of the classical swine fever virus E2 protein elicits neutralizing activity.

Amino Acid Sequence Animals Antibodies, Monoclonal / immunology Antibodies, Neutralizing / immunology Antibody Specificity / immunology Classical Swine Fever Virus / chemistry Conserved Sequence Epitopes / immunology Female Mice, Inbred BALB C Models, Molecular Peptide Library Viral Envelope Proteins / chemistry
Classical swine fever virus Linear B-cell epitope Neutralizing monoclonal antibodies

Journal

International journal of biological macromolecules
ISSN: 1879-0003
Titre abrégé: Int J Biol Macromol
Pays: Netherlands
ID NLM: 7909578

Informations de publication

Date de publication:
31 Oct 2021
Historique:
received: 16 02 2021
revised: 07 08 2021
accepted: 10 08 2021
pubmed: 18 8 2021
medline: 4 1 2022
entrez: 17 8 2021
Statut: ppublish

Résumé

Classical swine fever virus (CSFV) is a member of the genus Pestivirus, which causes serious economic losses. The re-emergence of the disease in Japan in 2018 has increased awareness of CSFV. In this study, Balb/c mice were immunized with plant-derived E2 protein, and four monoclonal antibodies (mAbs) 4B11, 7B3, 11A5 and 6F3 were generated. Two of these mAbs, 4B11 and 7B3, effectively blocked CSFV infection of PK-15 cells. Both mAbs recognized a novel linear epitope,

Identifiants

DOI: 10.1016/j.ijbiomac.2021.08.088 PMID: 34403672
pubmed: 34403672
pii: S0141-8130(21)01747-5
doi: 10.1016/j.ijbiomac.2021.08.088
pii:
doi:

Substances chimiques

Antibodies, Monoclonal 0
Antibodies, Neutralizing 0
Epitopes 0
Peptide Library 0
Viral Envelope Proteins 0
glycoprotein E2, classical swine fever virus 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

837-846

Informations de copyright

Copyright © 2021 Elsevier B.V. All rights reserved.

Auteurs

Qianru Xu (Q)

College of Veterinary Medicine, Northwest A& F University, Yangling 712100, China; College of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, China; International Associated Research Center of National Animal Immunology, Zhengzhou 450046, China; Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China.

Junqing Guo (J)

Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China.

Fanshu Ma (F)

CAS Key Laboratory of Nano-Bio Interface, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou 215123, China.

Linke Liu (L)

College of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, China; International Associated Research Center of National Animal Immunology, Zhengzhou 450046, China.

Yanan Wang (Y)

College of Veterinary medicine, Jilin University, Changchun 130062, China.

Shenli Zhang (S)

College of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, China; International Associated Research Center of National Animal Immunology, Zhengzhou 450046, China.

Xiangxiang Niu (X)

College of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, China; International Associated Research Center of National Animal Immunology, Zhengzhou 450046, China.

Xueyang Li (X)

College of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, China; International Associated Research Center of National Animal Immunology, Zhengzhou 450046, China.

Min Jiang (M)

College of public health, Zhengzhou University, Zhengzhou 450001, China.

Yanwei Wang (Y)

College of public health, Zhengzhou University, Zhengzhou 450001, China.

Li Wang (L)

Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China.

Yunchao Liu (Y)

Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China.

Qingmei Li (Q)

Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China.

Shujun Chai (S)

Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China.

Ruining Wang (R)

College of veterinary Medicine, Henan University of Animal Husbandry and Economy, Zhengzhou 450044, China.

Qiang Ma (Q)

Institution of Animal Science & Veterinary Medicine, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China.

Erqin Zhang (E)

College of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, China; International Associated Research Center of National Animal Immunology, Zhengzhou 450046, China. Electronic address: zhangerqin76@163.com.

Gaiping Zhang (G)

College of Veterinary Medicine, Northwest A& F University, Yangling 712100, China; College of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, China; International Associated Research Center of National Animal Immunology, Zhengzhou 450046, China; Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China; Jiangsu Co-innovation Center for the Prevention and Control of Important Animal Infectious Disease and Zoonose, Yangzhou University, Yangzhou, China. Electronic address: zhanggaiping2003@163.com.

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Classifications MeSH

questionsmedicales.fr › Sciences de l'information › Sources d'information › Services d'information › Documentation › Données de séquences moléculaires › Séquence d'acides aminés › A novel linear epitope at the C-terminal...
questionsmedicales.fr › Eucaryotes › Animaux › A novel linear epitope at the C-terminal...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Globulines › Sérum-globulines › Immunoglobulines › Anticorps › Anticorps monoclonaux › A novel linear epitope at the C-terminal...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Globulines › Sérum-globulines › Immunoglobulines › Anticorps › Anticorps neutralisants › A novel linear epitope at the C-terminal...
questionsmedicales.fr › Phénomènes du système immunitaire › Spécificité des anticorps › A novel linear epitope at the C-terminal...
questionsmedicales.fr › Virus › Virus à ARN › Virus à ARN à brin positif › Flaviviridae › Pestivirus › Virus de la peste porcine classique › A novel linear epitope at the C-terminal...
questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Séquence conservée › A novel linear epitope at the C-terminal...
questionsmedicales.fr › Facteurs biologiques › Antigènes › Épitopes › A novel linear epitope at the C-terminal...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Souris › Lignées consanguines de souris › Souris de lignée BALB C › A novel linear epitope at the C-terminal...
questionsmedicales.fr › Techniques d'investigation › Modèles théoriques › Modèles moléculaires › A novel linear epitope at the C-terminal...
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Banque de gènes › Banque de peptides › A novel linear epitope at the C-terminal...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines virales › Protéines virales structurales › Protéines de l'enveloppe virale › A novel linear epitope at the C-terminal...