The dimeric form of CXCL12 binds to atypical chemokine receptor 1.
Journal
Science signaling
ISSN: 1937-9145
Titre abrégé: Sci Signal
Pays: United States
ID NLM: 101465400
Informations de publication
Date de publication:
17 08 2021
17 08 2021
Historique:
entrez:
18
8
2021
pubmed:
19
8
2021
medline:
15
1
2022
Statut:
epublish
Résumé
The pleiotropic chemokine CXCL12 is involved in diverse physiological and pathophysiological processes, including embryogenesis, hematopoiesis, leukocyte migration, and tumor metastasis. It is known to engage the classical receptor CXCR4 and the atypical receptor ACKR3. Differential receptor engagement can transduce distinct cellular signals and effects as well as alter the amount of free, extracellular chemokine. CXCR4 binds both monomeric and the more commonly found dimeric forms of CXCL12, whereas ACKR3 binds monomeric forms. Here, we found that CXCL12 also bound to the atypical receptor ACKR1 (previously known as Duffy antigen/receptor for chemokines or DARC). In vitro nuclear magnetic resonance spectroscopy and isothermal titration calorimetry revealed that dimeric CXCL12 bound to the extracellular N terminus of ACKR1 with low nanomolar affinity, whereas the binding affinity of monomeric CXCL12 was orders of magnitude lower. In transfected MDCK cells and primary human Duffy-positive erythrocytes, a dimeric, but not a monomeric, construct of CXCL12 efficiently bound to and internalized with ACKR1. This interaction between CXCL12 and ACKR1 provides another layer of regulation of the multiple biological functions of CXCL12. The findings also raise the possibility that ACKR1 can bind other dimeric chemokines, thus potentially further expanding the role of ACKR1 in chemokine retention and presentation.
Identifiants
pubmed: 34404752
pii: 14/696/eabc9012
doi: 10.1126/scisignal.abc9012
pmc: PMC9015690
mid: NIHMS1796055
pii:
doi:
Substances chimiques
ACKR1 protein, human
0
CXCL12 protein, human
0
Chemokine CXCL12
0
Duffy Blood-Group System
0
Receptors, CXCR4
0
Receptors, Cell Surface
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : NIAID NIH HHS
ID : R37 AI058072
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI058072
Pays : United States
Organisme : Wellcome Trust
ID : 200817/Z/16/Z
Pays : United Kingdom
Organisme : NIH HHS
ID : S10 OD020000
Pays : United States
Organisme : Versus Arthritis
Pays : United Kingdom
Organisme : NIGMS NIH HHS
ID : T32 GM080202
Pays : United States
Organisme : Wellcome Trust
Pays : United Kingdom
Informations de copyright
Copyright © 2021 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works.
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