En route to dynamic life processes by SNARE-mediated fusion of polymer and hybrid membranes.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
17 08 2021
17 08 2021
Historique:
received:
19
02
2021
accepted:
30
07
2021
entrez:
18
8
2021
pubmed:
19
8
2021
medline:
31
8
2021
Statut:
epublish
Résumé
A variety of artificial cells springs from the functionalization of liposomes with proteins. However, these models suffer from low durability without repair and replenishment mechanisms, which can be partly addressed by replacing the lipids with polymers. Yet natural membranes are also dynamically remodeled in multiple cellular processes. Here, we show that synthetic amphiphile membranes also undergo fusion, mediated by the protein machinery for synaptic secretion. We integrated fusogenic SNAREs in polymer and hybrid vesicles and observed efficient membrane and content mixing. We determined bending rigidity and pore edge tension as key parameters for fusion and described its plausible progression through cryo-EM snapshots. These findings demonstrate that dynamic membrane phenomena can be reconstituted in synthetic materials, thereby providing new tools for the assembly of synthetic protocells.
Identifiants
pubmed: 34404795
doi: 10.1038/s41467-021-25294-z
pii: 10.1038/s41467-021-25294-z
pmc: PMC8371082
doi:
Substances chimiques
Liposomes
0
Nerve Tissue Proteins
0
Polymers
0
R-SNARE Proteins
0
SNARE Proteins
0
Synaptosomal-Associated Protein 25
0
Syntaxin 1
0
Vesicle-Associated Membrane Protein 2
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
4972Subventions
Organisme : NIGMS NIH HHS
ID : P01 GM072694
Pays : United States
Informations de copyright
© 2021. The Author(s).
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