Revealing the influence of electron beam melted Ti-6Al-4V scaffolds on osteogenesis of human bone marrow-derived mesenchymal stromal cells.
Alkaline Phosphatase
/ metabolism
Alloys
/ chemistry
Biocompatible Materials
/ chemistry
Bone Marrow
/ metabolism
Bone Substitutes
Bone and Bones
/ metabolism
Calcification, Physiologic
Calcium
/ metabolism
Cell Differentiation
Cell Survival
Collagen Type I, alpha 1 Chain
/ metabolism
Core Binding Factor Alpha 1 Subunit
/ metabolism
Electrons
Humans
Mesenchymal Stem Cells
/ cytology
Osteoblasts
/ metabolism
Osteogenesis
/ drug effects
Porosity
Prostheses and Implants
Prosthesis Design
Stromal Cells
/ metabolism
Tissue Scaffolds
/ chemistry
Titanium
/ chemistry
Journal
Journal of materials science. Materials in medicine
ISSN: 1573-4838
Titre abrégé: J Mater Sci Mater Med
Pays: United States
ID NLM: 9013087
Informations de publication
Date de publication:
18 Aug 2021
18 Aug 2021
Historique:
received:
19
04
2021
accepted:
23
07
2021
entrez:
18
8
2021
pubmed:
19
8
2021
medline:
19
1
2022
Statut:
epublish
Résumé
Porous Titanium-6Aluminum-4Vanadium scaffolds made by electron beam-based additive manufacturing (AM) have emerged as state-of-the-art implant devices. However, there is still limited knowledge on how they influence the osteogenic differentiation of bone marrow-derived mesenchymal stromal cells (BMSCs). In this study, BMSCs are cultured on such porous scaffolds to determine how the scaffolds influence the osteogenic differentiation of the cells. The scaffolds are biocompatible, as revealed by the increasing cell viability. Cells are evenly distributed on the scaffolds after 3 days of culturing followed by an increase in bone matrix development after 21 days of culturing. qPCR analysis provides insight into the cells' osteogenic differentiation, where RUNX2 expression indicate the onset of differentiation towards osteoblasts. The COL1A1 expression suggests that the differentiated osteoblasts can produce the osteoid. Alkaline phosphatase staining indicates an onset of mineralization at day 7 in OM. The even deposits of calcium at day 21 further supports a successful bone mineralization. This work shines light on the interplay between AM Ti64 scaffolds and bone growth, which may ultimately lead to a new way of creating long lasting bone implants with fast recovery times.
Identifiants
pubmed: 34406475
doi: 10.1007/s10856-021-06572-0
pii: 10.1007/s10856-021-06572-0
pmc: PMC8373740
doi:
Substances chimiques
Alloys
0
Biocompatible Materials
0
Bone Substitutes
0
Collagen Type I, alpha 1 Chain
0
Core Binding Factor Alpha 1 Subunit
0
RUNX2 protein, human
0
titanium alloy (TiAl6V4)
12743-70-3
Titanium
D1JT611TNE
Alkaline Phosphatase
EC 3.1.3.1
Calcium
SY7Q814VUP
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
97Informations de copyright
© 2021. The Author(s).
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