A Photoacoustic Contrast Agent for miR-21 via NIR Fluorescent Hybridization Chain Reaction.
Journal
Bioconjugate chemistry
ISSN: 1520-4812
Titre abrégé: Bioconjug Chem
Pays: United States
ID NLM: 9010319
Informations de publication
Date de publication:
15 06 2022
15 06 2022
Historique:
pubmed:
19
8
2021
medline:
18
6
2022
entrez:
18
8
2021
Statut:
ppublish
Résumé
Nucleic acids are well-established biomarkers of cancer with immense value in diagnostics and basic research. However, strategies to monitor these species in tissue can be challenging due to the need for amplification of imaging signal from low analyte concentrations with high specificity. Photoacoustic (PA) imaging is gaining traction for molecular imaging of proteins, small biomolecules, and nucleic acids by coupling pulsed near-infrared (NIR) excitation with broadband acoustic detection. This work introduces a PA nucleic acid contrast agent that harnesses NIR fluorophore and quencher-tagged hybridization chain reaction (HCR) for signal amplification. This HCR probe was designed to enable contact quenching between NIR dye-quencher pairs by coercing their direct alignment when miR-21, a microRNA cancer biomarker, is detected. The probe demonstrated a ratiometric PA limit of detection of 148 pM miR-21, sequence specificity against one- and two-base mutations, and selectivity over other microRNAs. It was further tested in live human ovarian cancer (SKOV3) and noncancerous (HEK 293T) cells to exemplify
Identifiants
pubmed: 34406744
doi: 10.1021/acs.bioconjchem.1c00375
pmc: PMC9122088
mid: NIHMS1803436
doi:
Substances chimiques
Contrast Media
0
Fluorescent Dyes
0
MIRN21 microRNA, human
0
MicroRNAs
0
DNA
9007-49-2
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
1080-1092Subventions
Organisme : NHLBI NIH HHS
ID : DP2 HL137187
Pays : United States
Organisme : NIAID NIH HHS
ID : R21 AI157957
Pays : United States
Organisme : NIH HHS
ID : S10 OD021821
Pays : United States
Organisme : NCI NIH HHS
ID : T32 CA153915
Pays : United States
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