Effects of mechanical strain on periodontal ligament fibroblasts in presence of Aggregatibacter actinomycetemcomitans lysate.
Inflammation
Orthodontic tooth movement
PDL
Periodontal ligament fibroblast
Periodontitis
Journal
BMC oral health
ISSN: 1472-6831
Titre abrégé: BMC Oral Health
Pays: England
ID NLM: 101088684
Informations de publication
Date de publication:
18 08 2021
18 08 2021
Historique:
received:
07
04
2021
accepted:
04
08
2021
entrez:
19
8
2021
pubmed:
20
8
2021
medline:
2
10
2021
Statut:
epublish
Résumé
Many adult orthodontic patients suffer from periodontitis, which is caused by oral pathogens such as the gram-negative Aggregatibacter actinomycetemcomitans (Agac). Like orthodontic tooth movement, periodontitis is associated with inflammation and alveolar bone remodelling thereby affecting orthodontic treatment. Interactions of both processes, however, are not sufficiently explored, particularly with regard to oxidative stress. After preincubation with Agac lysate for 24 h periodontal ligament fibroblasts (PDLF) were either stretched or compressed for further 48 h simulating orthodontic forces in vitro. We analysed the expression of genes and proteins involved in the formation of reactive oxygen species (NOX-4, ROS) and nitric oxide (NOS-2), inflammation (TNF, IL-6, PTGS-2) and bone remodelling (OPG, RANKL). Agac lysate elevated the expression of NOX-4, NOS-2, inflammatory IL-6 and PTGS-2 and the bone-remodelling RANKL/OPG ratio during compressive, but not tensile mechanical strain. Agac lysate stimulated pressure-induced inflammatory signalling, whereas surprisingly ROS formation was reduced. Pressure-induced downregulation of OPG expression was inhibited by Agac lysate. Agac lysate impact on the expression of genes and proteins involved in inflammation and bone remodelling as well as ROS formation, when PDLF were subjected to mechanical forces occurring during orthodontic tooth movement.
Identifiants
pubmed: 34407790
doi: 10.1186/s12903-021-01761-3
pii: 10.1186/s12903-021-01761-3
pmc: PMC8371899
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
405Informations de copyright
© 2021. The Author(s).
Références
J Periodontol. 2010 Oct;81(10):1488-96
pubmed: 20528699
Eur J Orthod. 2020 Sep 11;42(4):359-370
pubmed: 31352484
Eur J Oral Sci. 2007 Oct;115(5):355-62
pubmed: 17850423
Alcohol. 2019 Jun;77:59-70
pubmed: 30336201
Open Access Maced J Med Sci. 2019 Jul 20;7(14):2343-2349
pubmed: 31592049
Sci Rep. 2017 Nov 7;7(1):14751
pubmed: 29116140
Am J Orthod Dentofacial Orthop. 2014 Nov;146(5):620-32
pubmed: 25439213
J Clin Periodontol. 2009 Jul;36 Suppl 10:3-8
pubmed: 19432625
Clin Oral Investig. 2013 Dec;17(9):2041-8
pubmed: 23392729
J Dent Res. 2017 Apr;96(4):361-363
pubmed: 28318392
Infect Immun. 2017 Jan 26;85(2):
pubmed: 27849181
Indian J Endocrinol Metab. 2011 Jul;15(3):175-81
pubmed: 21897893
J Bone Miner Res. 2002 Feb;17(2):210-20
pubmed: 11811551
Eur J Orthod. 2006 Jun;28(3):221-40
pubmed: 16687469
Odontology. 2019 Apr;107(2):133-141
pubmed: 29959559
Innate Immun. 2016 Nov;22(8):674-681
pubmed: 28071181
Oral Microbiol Immunol. 2006 Dec;21(6):360-5
pubmed: 17064393
Front Oral Biol. 2016;18:75-9
pubmed: 26599120
Arch Oral Biol. 2009 Jul;54(7):618-24
pubmed: 19406381
Exp Physiol. 1997 Mar;82(2):291-5
pubmed: 9129943
Cells. 2021 Feb 01;10(2):
pubmed: 33535605
Mediators Inflamm. 2019 Mar 10;2019:2514956
pubmed: 30983880
J Orofac Orthop. 2014 May;75(3):191-202
pubmed: 24825831
Periodontol 2000. 2007;43:160-232
pubmed: 17214840
J Orofac Orthop. 2018 Sep;79(5):337-351
pubmed: 30019109
Kaohsiung J Med Sci. 2018 Apr;34(4):207-214
pubmed: 29655409
J Orofac Orthop. 2020 Sep;81(5):360-370
pubmed: 32632652
Periodontol 2000. 2015 Oct;69(1):7-17
pubmed: 26252398
Front Physiol. 2017 May 30;8:351
pubmed: 28611683
J Immunol. 2004 Sep 15;173(6):3589-93
pubmed: 15356101
Orthod Craniofac Res. 2009 May;12(2):113-9
pubmed: 19419454
Periodontol 2000. 2006;40:11-28
pubmed: 16398683
Lancet. 2005 Nov 19;366(9499):1809-20
pubmed: 16298220
Proc Natl Acad Sci U S A. 2000 Jul 5;97(14):7993-8
pubmed: 10869429
Oral Dis. 2016 Oct;22(7):609-19
pubmed: 26704694
J Public Health Dent. 2017 Dec;77(1):3-5
pubmed: 28276588
J Periodontol. 2011 Nov;82(11):1608-15
pubmed: 21417589
Inflamm Regen. 2020 Feb 7;40:2
pubmed: 32047573
Orthod Craniofac Res. 2006 May;9(2):63-70
pubmed: 16764680
Cell Metab. 2015 Mar 3;21(3):493-501
pubmed: 25738463
Methods. 2001 Dec;25(4):402-8
pubmed: 11846609
J Am Dent Assoc. 2008 Apr;139(4):413-22
pubmed: 18385025
J Clin Periodontol. 2019 Jun;46(6):608-622
pubmed: 30989678
Biomed Res Int. 2019 May 23;2019:5063565
pubmed: 31240214
J Dent Res. 2007 Nov;86(11):1089-94
pubmed: 17959902
Ann Anat. 2017 Mar;210:32-43
pubmed: 27838559
Eur J Oral Sci. 2008 Apr;116(2):89-97
pubmed: 18353001
Am J Orthod Dentofacial Orthop. 2001 Mar;119(3):307-12
pubmed: 11244425
J Dent Res. 1996 Jan;75(1):562-7
pubmed: 8655760
Periodontol 2000. 2001;25:21-36
pubmed: 11155180
Front Physiol. 2017 Dec 14;8:1055
pubmed: 29311982
Mediators Inflamm. 2020 Apr 28;2020:2814015
pubmed: 32410848
Periodontol 2000. 1997 Feb;13:76-90
pubmed: 9567924