Spectroscopic label-free microscopy of changes in live cell chromatin and biochemical composition in transplantable organoids.
Journal
Science advances
ISSN: 2375-2548
Titre abrégé: Sci Adv
Pays: United States
ID NLM: 101653440
Informations de publication
Date de publication:
08 2021
08 2021
Historique:
received:
03
05
2021
accepted:
30
06
2021
entrez:
19
8
2021
pubmed:
20
8
2021
medline:
16
4
2022
Statut:
epublish
Résumé
Organoids formed from human induced pluripotent stem cells (hiPSCs) could be a limitless source of functional tissue for transplantations in many organs. Unfortunately, fine-tuning differentiation protocols to form large quantities of hiPSC organoids in a controlled, scalable, and reproducible manner is quite difficult and often takes a very long time. Recently, we introduced a new approach of rapid organoid formation from dissociated hiPSCs and endothelial cells using microfabricated cell-repellent microwell arrays. This approach, when combined with real-time label-free Raman spectroscopy of biochemical composition changes and confocal light scattering spectroscopic microscopy of chromatin transition, allows for monitoring live differentiating organoids without the need to sacrifice a sample, substantially shortening the time of protocol fine-tuning. We used this approach to both culture and monitor homogeneous liver organoids that have the main functional features of the human liver and which could be used for cell transplantation liver therapy in humans.
Identifiants
pubmed: 34407934
pii: 7/34/eabj2800
doi: 10.1126/sciadv.abj2800
pmc: PMC8373132
pii:
doi:
Substances chimiques
Chromatin
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : NCI NIH HHS
ID : R01 CA214893
Pays : United States
Organisme : NCI NIH HHS
ID : R01 CA205431
Pays : United States
Organisme : NCI NIH HHS
ID : R01 CA218382
Pays : United States
Organisme : NIBIB NIH HHS
ID : R01 EB003472
Pays : United States
Organisme : NIBIB NIH HHS
ID : R01 EB025173
Pays : United States
Organisme : NCI NIH HHS
ID : R01 CA228029
Pays : United States
Informations de copyright
Copyright © 2021 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).
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