DNA damage in circulating leukocytes measured with the comet assay may predict the risk of death.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
18 08 2021
18 08 2021
Historique:
received:
31
03
2021
accepted:
02
08
2021
entrez:
19
8
2021
pubmed:
20
8
2021
medline:
11
11
2021
Statut:
epublish
Résumé
The comet assay or single cell gel electrophoresis, is the most common method used to measure strand breaks and a variety of other DNA lesions in human populations. To estimate the risk of overall mortality, mortality by cause, and cancer incidence associated to DNA damage, a cohort of 2,403 healthy individuals (25,978 person-years) screened in 16 laboratories using the comet assay between 1996 and 2016 was followed-up. Kaplan-Meier analysis indicated a worse overall survival in the medium and high tertile of DNA damage (p < 0.001). The effect of DNA damage on survival was modelled according to Cox proportional hazard regression model. The adjusted hazard ratio (HR) was 1.42 (1.06-1.90) for overall mortality, and 1.94 (1.04-3.59) for diseases of the circulatory system in subjects with the highest tertile of DNA damage. The findings of this study provide epidemiological evidence encouraging the implementation of the comet assay in preventive strategies for non-communicable diseases.
Identifiants
pubmed: 34408182
doi: 10.1038/s41598-021-95976-7
pii: 10.1038/s41598-021-95976-7
pmc: PMC8373872
doi:
Substances chimiques
Cell-Free Nucleic Acids
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
16793Subventions
Organisme : NCI NIH HHS
ID : P30 CA008748
Pays : United States
Investigateurs
Diana Anderson
(D)
Vanessa Andrade
(V)
Cristiana Costa Pereira
(CC)
Solange Costa
(S)
Kristine B Gutzkow
(KB)
Carina Ladeira
(C)
Massimo Moretti
(M)
Carla Costa
(C)
Irene Orlow
(I)
Emilio Rojas
(E)
Bertrand Pourrut
(B)
Marcin Kruszewski
(M)
Siegfried Knasmueller
(S)
Sergey Shaposhnikov
(S)
Bojana Žegura
(B)
Helga Stopper
(H)
Commentaires et corrections
Type : ErratumIn
Informations de copyright
© 2021. The Author(s).
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