Mapping of serological testing and SARS-CoV-2 seroprevalence studies performed in 20 European countries, March-June 2020.


Journal

Journal of global health
ISSN: 2047-2986
Titre abrégé: J Glob Health
Pays: Scotland
ID NLM: 101578780

Informations de publication

Date de publication:
2021
Historique:
entrez: 19 8 2021
pubmed: 20 8 2021
medline: 21 8 2021
Statut: epublish

Résumé

The SARS-CoV-2 pandemic spread across Europe from February 2020. While robust SARS-CoV-2 serological assays were quickly developed, only limited information on applied serological testing is available. We describe the extent and nature of SARS-CoV-2 serological testing used in Europe and assess the links between epidemiology, mitigation strategies applied and seroprevalence. An online questionnaire on SARS-CoV-2 serology was sent to the European Society of Clinical Virology and European Non-Polio Enterovirus Network members in September 2020. Data were analysed by comparing mitigation approaches, serological methods and seroprevalance studies performed. About 100 000 laboratory confirmed cases identified between March and June 2020 were reported by 36 participating laboratories from 20 countries. All responders experienced mitigation strategies including lockdowns and other closures. All except one participant had introduced serological testing; most had validated their assays (n = 29), but some had had difficulties in obtaining reference material. Most used commercial assays (n = 35), measuring IgG response against the spike antigen. Serology was used primarily for diagnostic purposes (n = 22) but also for convalescent plasma (n = 13) and research studies (n = 30). Seroprevalence studies targeted mainly health care workers (n = 20; seroprevalance 5% to 22%) and general population (n = 16; seroprevalance 0.88% to 5.6%). Basic demographic and clinical information were collected by most laboratories (n = 28), whereas data on long-term outcomes were rarely collected. This is first study gathering systematic information on serological testing approaches implemented during the first pandemic wave in Europe.

Sections du résumé

BACKGROUND BACKGROUND
The SARS-CoV-2 pandemic spread across Europe from February 2020. While robust SARS-CoV-2 serological assays were quickly developed, only limited information on applied serological testing is available. We describe the extent and nature of SARS-CoV-2 serological testing used in Europe and assess the links between epidemiology, mitigation strategies applied and seroprevalence.
METHODS METHODS
An online questionnaire on SARS-CoV-2 serology was sent to the European Society of Clinical Virology and European Non-Polio Enterovirus Network members in September 2020. Data were analysed by comparing mitigation approaches, serological methods and seroprevalance studies performed.
RESULTS RESULTS
About 100 000 laboratory confirmed cases identified between March and June 2020 were reported by 36 participating laboratories from 20 countries. All responders experienced mitigation strategies including lockdowns and other closures. All except one participant had introduced serological testing; most had validated their assays (n = 29), but some had had difficulties in obtaining reference material. Most used commercial assays (n = 35), measuring IgG response against the spike antigen. Serology was used primarily for diagnostic purposes (n = 22) but also for convalescent plasma (n = 13) and research studies (n = 30). Seroprevalence studies targeted mainly health care workers (n = 20; seroprevalance 5% to 22%) and general population (n = 16; seroprevalance 0.88% to 5.6%). Basic demographic and clinical information were collected by most laboratories (n = 28), whereas data on long-term outcomes were rarely collected.
CONCLUSIONS CONCLUSIONS
This is first study gathering systematic information on serological testing approaches implemented during the first pandemic wave in Europe.

Identifiants

pubmed: 34408857
doi: 10.7189/jogh.11.05014
pii: jogh-11-05014
pmc: PMC8364255
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

05014

Informations de copyright

Copyright © 2021 by the Journal of Global Health. All rights reserved.

Déclaration de conflit d'intérêts

Competing interests: The authors completed the ICME Unified Competing Interest form (available upon request from the corresponding author) and declare no conflict of interest.

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Auteurs

Laura Bubba (L)

Department of Biomedical Science for Health, University of Milan, Milan, Italy.

Peter Simmonds (P)

Nuffield Department of Medicine, University of Oxford, Oxford, UK.

Thea K Fischer (TK)

Department of Clinical Research, University hospital of Nordsjaelland, Hilleroed, Denmark.
Department of Public Health, University of Copenhagen, Copenhagen, Denmark.

Heli Harvala (H)

Microbiology Services, NHS Blood and Transplant, London, UK.
Division of Infection and Immunity, University College London, London, UK.

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Classifications MeSH