Rapid Base-Specific Calling of SARS-CoV-2 Variants of Concern Using Combined RT-PCR Melting Curve Screening and SIRPH Technology.
SARS-CoV-2
SIRPH
SNuPE
mutation screening
variants of concern
Journal
Open forum infectious diseases
ISSN: 2328-8957
Titre abrégé: Open Forum Infect Dis
Pays: United States
ID NLM: 101637045
Informations de publication
Date de publication:
Aug 2021
Aug 2021
Historique:
received:
14
04
2021
accepted:
12
07
2021
entrez:
19
8
2021
pubmed:
20
8
2021
medline:
20
8
2021
Statut:
epublish
Résumé
The emergence of novel variants of concern of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) demands fast and reliable detection of such variants in local populations. Here we present a cost-efficient and fast workflow combining a prescreening of SARS-CoV-2-positive samples using reverse transcription polymerase chain reaction melting curve analysis with multiplexed IP-RP-HPLC-based single nucleotide primer extensions. The entire workflow from positive SARS-CoV-2 testing to base-specific identification of variants requires about 24 hours. We applied the sensitive method to monitor local variant of concern outbreaks in SARS-CoV-2-positive samples collected in a confined region of Germany.
Sections du résumé
BACKGROUND
BACKGROUND
The emergence of novel variants of concern of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) demands fast and reliable detection of such variants in local populations.
METHODS
METHODS
Here we present a cost-efficient and fast workflow combining a prescreening of SARS-CoV-2-positive samples using reverse transcription polymerase chain reaction melting curve analysis with multiplexed IP-RP-HPLC-based single nucleotide primer extensions.
RESULTS
RESULTS
The entire workflow from positive SARS-CoV-2 testing to base-specific identification of variants requires about 24 hours.
CONCLUSIONS
CONCLUSIONS
We applied the sensitive method to monitor local variant of concern outbreaks in SARS-CoV-2-positive samples collected in a confined region of Germany.
Identifiants
pubmed: 34409122
doi: 10.1093/ofid/ofab364
pii: ofab364
pmc: PMC8364759
doi:
Types de publication
Journal Article
Langues
eng
Pagination
ofab364Informations de copyright
© The Author(s) 2021. Published by Oxford University Press on behalf of Infectious Diseases Society of America.
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