Novel ketogenic diet formulation improves sucrose-induced insulin resistance in canton strain Drosophila melanogaster.

Drosophila melanogaster glucose intolerance high sucrose diet insulin resistance ketogenic diet type-2 diabetes mellitus

Journal

Journal of food biochemistry
ISSN: 1745-4514
Titre abrégé: J Food Biochem
Pays: United States
ID NLM: 7706045

Informations de publication

Date de publication:
09 2021
Historique:
revised: 06 08 2021
received: 09 06 2021
accepted: 07 08 2021
pubmed: 20 8 2021
medline: 29 10 2021
entrez: 19 8 2021
Statut: ppublish

Résumé

This study investigates the antidiabetic effect of a ketogenic diet (KD) on sucrose-induced insulin resistance in the fruit fly model. The fruit flies were divided and grouped into four: Group A, B, C, and D, representing the control, high-sucrose diet (HSD), KD, and HSD + KD, respectively. The administration of the various treatments to the groups proceeded for 7 days. The flies were thereafter immobilized, homogenized, and the homogenates used for biochemical parameters determination. This includes glucose concentration, antioxidant status, 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase, total cholesterol (TC), triglycerides (TG), and protein concentration. There was a significant increase (p < .05) in weight gain, glucose concentration, TG, HMG-CoA reductase activity, TC, and lipid peroxidation status of the HSD group compared with the control and KD groups. The antioxidant enzymes measured (superoxide dismutase, catalase, and reduced glutathione) and protein concentrations were repressed significantly (p < .05) in the HD groups but significantly elevated (p < .05) in the KD, HSD + KD, and the control groups. The KD improved biochemical parameters altered during the onset of sucrose-induced insulin resistance. With further research on this, KD may emerge as the much-awaited treatment option for diabetes mellitus type 2 (T2DM) with almost reduced toxicity concerns. PRACTICAL APPLICATIONS: Novel KD are sources of dietary phytocompounds with proven antioxidant activities. The antidiabetic activity of the KD was investigated. The results showed that the KD proves to serve as a better effective antidiabetic option in Drosophila melanogaster. The observed results could provide the potential application of the KD as an alternative therapy for diabetes management.

Identifiants

pubmed: 34409649
doi: 10.1111/jfbc.13907
doi:

Substances chimiques

Antioxidants 0
Sucrose 57-50-1

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

e13907

Informations de copyright

© 2021 Wiley Periodicals LLC.

Références

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Auteurs

Omowumi T Kayode (OT)

Biochemistry Unit, Department of Biological Sciences, Mountain Top University, Prayercity, Ogun State, Nigeria.

Damilare Rotimi (D)

Medicinal Biochemistry and Molecular Toxicology Group, Department of Biochemistry, Landmark University, Omu-Aran, Nigeria.

Elizabeth Okoh (E)

Medicinal Biochemistry and Molecular Toxicology Group, Department of Biochemistry, Landmark University, Omu-Aran, Nigeria.

Matthew Iyobhebhe (M)

Medicinal Biochemistry and Molecular Toxicology Group, Department of Biochemistry, Landmark University, Omu-Aran, Nigeria.

Abolanle A A Kayode (AAA)

Department of Biochemistry, Benjamin Carson School of Medicine, Babcock University, Ilishan Remo, Ogun State, Nigeria.

Oluwafemi Adeleke Ojo (OA)

Medicinal Biochemistry and Molecular Toxicology Group, Department of Biochemistry, Landmark University, Omu-Aran, Nigeria.

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