The active ingredients analysis of Herba Lysimachiae treating osteoarthritis based on the LC-MS/MS technology and public bioinformatics platforms.
Herba Lysimachiae
active ingredients
bioinformatics
liquid chromatography-tandem mass spectrometry
osteoarthritis
Journal
Journal of separation science
ISSN: 1615-9314
Titre abrégé: J Sep Sci
Pays: Germany
ID NLM: 101088554
Informations de publication
Date de publication:
Oct 2021
Oct 2021
Historique:
revised:
12
08
2021
received:
13
05
2021
accepted:
15
08
2021
pubmed:
20
8
2021
medline:
1
2
2022
entrez:
19
8
2021
Statut:
ppublish
Résumé
Herba Lysimachiae inhibits synovial damage in osteoarthritis via regulating two bio labels (integrin alpha 2b/beta 3). However, the relevant active ingredients are still unknown. Here, the active ingredients of herbal medicines were analyzed based on the liquid chromatography-tandem mass spectrometry technology and public bioinformatics platforms. The liquid chromatography-tandem mass spectrometry technology was used for compound analysis, and public databases (PubChem BioAssay and STRING) were applied to establish the links between herbal compounds and both bio labels, and identify which herbal compounds may regulate these bio labels. Subsequently, the osteoarthritis model was used to confirm the results. Totally, ninety compounds in Herba Lysimachiae were identified based on the liquid chromatography-tandem mass spectrometry technology. Bioinformatics analysis showed that five compounds (myricetin, fisetin, esculetin, 7-hydroxycoumarin-4-acetic acid, and caffeic acid) may synergistically regulate bio labels through 11 targets, which may be the active ingredients of Herba Lysimachiae for osteoarthritis treatment. In the verification experiments, five compounds markedly suppressed the overexpression of bio labels in the synovium of the osteoarthritis model. In conclusion, the present study effectively and rapidly analyzed the active ingredients of Herba Lysimachiae for osteoarthritis treatment.
Identifiants
pubmed: 34409742
doi: 10.1002/jssc.202100390
doi:
Substances chimiques
Drugs, Chinese Herbal
0
Iodoacetic Acid
WF5188V710
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
3799-3809Subventions
Organisme : National Natural Science Foundation of China
ID : 81760784
Organisme : Science and Technology Project of Guizhou Province
ID : QKH Foundation Project [2019]1037
Organisme : High-level Talents Project of Guizhou Province
ID : GUTCM[ZQ2018003]
Informations de copyright
© 2021 Wiley-VCH GmbH.
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