Antimalarial drug resistance in the Central and Adamawa regions of Cameroon: Prevalence of mutations in P. falciparum crt, Pfmdr1, Pfdhfr and Pfdhps genes.
Journal
PloS one
ISSN: 1932-6203
Titre abrégé: PLoS One
Pays: United States
ID NLM: 101285081
Informations de publication
Date de publication:
2021
2021
Historique:
received:
30
01
2021
accepted:
05
08
2021
entrez:
19
8
2021
pubmed:
20
8
2021
medline:
17
12
2021
Statut:
epublish
Résumé
The spread of Plasmodium falciparum resistant parasites remains one of the major challenges for malaria control and elimination in Sub Saharan Africa. Monitoring of molecular markers conferring resistance to different antimalarials is important to track the spread of resistant parasites and to optimize the therapeutic lifespan of current drugs. This study aimed to evaluate the prevalence of known mutations in the drug resistance genes Pfcrt, Pfmdr1, Pfdhfr and Pfdhps in two different epidemiological settings in Cameroon. Dried blood spots collected in 2018 and 2019 from asymptomatic individuals were used for DNA extraction and then the Plasmodium infection status was determined byPCR. Detection of SNPs was performed by nested PCR followed by allele-specific restriction analysis (ASRA). The prevalence of each genotype was compared between sites using the Chi square and Fisher's exact tests. A high prevalence of the Pfcrt K76 wild type allele was found in both sites (88.5 and 62.29% respectively; P< 0,0001). The prevalence of Pfmdr1 mutations 86Y and 1246Y was respectively 55.83 and 1.45% in Mfou and 45.87 and 5.97% in Tibati, with significant difference between the studied areas (P<0.0001). Overall, the Pfdhfr triple-mutant genotype (51I/59R/108N) was highly prevalent (> 96%), however no SNP was detected at codon 164. In Pfdhps, the prevalence of the 437G mutation reached (90%) and was at higher frequency in Mfou (P< 0.0001). Overall, the Pfdhps mutations 540E and 581G were less common (0.33 and 3.26%, respectively). The quadruple resistant genotype (Pfdhfr 51I/59R/108N+Pfdhp437G) was found almost 90% of the samples. The wild-type genotype (Pfdhfr N51/C59/S108/164I+Pfdhps A437/K540/A581) was never identified and the sextuple mutant (Pfdhfr 51I/59R/108N+Pfdhp437G/540E/581G), kwon as super resistant appeared in two samples from Tibati. These findings demonstrate declining trends in the prevalence of mutations conferring resistance to 4-aminoquinolines, especially to chloroquine. However, a high level of mutations in P. falciparum genes related to SP resistance was detected and this raises concerns about the future efficacy of IPTp-SP and SMC in Cameroon.
Identifiants
pubmed: 34411157
doi: 10.1371/journal.pone.0256343
pii: PONE-D-21-03307
pmc: PMC8376100
doi:
Substances chimiques
Antimalarials
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0256343Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
Références
Malar J. 2018 Nov 7;17(1):413
pubmed: 30404640
Parasitology. 2001 May;122(Pt 5):497-505
pubmed: 11393822
Antimicrob Agents Chemother. 2019 Apr 25;63(5):
pubmed: 30803964
J Infect Dis. 2005 Mar 15;191(6):1014-7
pubmed: 15717281
Malar J. 2020 Mar 2;19(1):100
pubmed: 32122339
Infect Drug Resist. 2018 Aug 28;11:1329-1338
pubmed: 30214253
Malar J. 2012 Oct 26;11:355
pubmed: 23101492
Parasite. 2016;23:28
pubmed: 27443837
Lancet. 2012 May 26;379(9830):1960-6
pubmed: 22484134
Malar J. 2014 Apr 21;13:152
pubmed: 24751352
Trop Med Int Health. 2008 Feb;13(2):238-40
pubmed: 18304270
Infect Genet Evol. 2007 Sep;7(5):562-9
pubmed: 17467344
PLoS One. 2013;8(3):e57689
pubmed: 23520478
N Engl J Med. 2014 Jul 31;371(5):397-9
pubmed: 25075832
J Clin Microbiol. 2005 May;43(5):2435-40
pubmed: 15872277
Am J Trop Med Hyg. 2002 Jun;66(6):667-71
pubmed: 12224572
Mol Cell. 2000 Oct;6(4):861-71
pubmed: 11090624
Am J Trop Med Hyg. 2014 Jul;91(1):54-61
pubmed: 24799371
Clin Infect Dis. 2016 Feb 1;62(3):323-333
pubmed: 26486699
Expert Rev Anti Infect Ther. 2013 Jun;11(6):623-39
pubmed: 23750733
PLoS Pathog. 2015 Apr 02;11(4):e1004789
pubmed: 25836766
Malar J. 2017 Jan 13;16(1):28
pubmed: 28086777
Malar J. 2020 Oct 22;19(1):378
pubmed: 33092587
Malar J. 2009 Jul 29;8:176
pubmed: 19640292
Diagn Pathol. 2015 Apr 28;10:41
pubmed: 25928117
Int J Parasitol Drugs Drug Resist. 2016 Dec;6(3):220-229
pubmed: 27821281
J Infect Dis. 2001 Jun 15;183(12):1828-31
pubmed: 11372041
Infect Genet Evol. 2011 Mar;11(2):512-7
pubmed: 21251998
Indian J Psychol Med. 2013 Apr;35(2):121-6
pubmed: 24049221
Infect Dis Poverty. 2020 Jul 11;9(1):91
pubmed: 32653033
Malar J. 2016 Aug 18;15(1):420
pubmed: 27538948
BMC Res Notes. 2019 Apr 23;12(1):235
pubmed: 31014391
PLoS One. 2019 Sep 3;14(9):e0221895
pubmed: 31479501
Infect Dis Poverty. 2017 Nov 6;6(1):136
pubmed: 29110722
Sci Rep. 2017 Aug 7;7(1):7389
pubmed: 28785011
Parasit Vectors. 2019 Oct 26;12(1):501
pubmed: 31655608
Malar J. 2021 Feb 5;20(1):72
pubmed: 33546703
Malar J. 2019 Mar 26;18(1):101
pubmed: 30914041
N Engl J Med. 2001 Jan 25;344(4):257-63
pubmed: 11172152
Malar J. 2020 Nov 30;19(1):439
pubmed: 33256739
Trends Parasitol. 2013 Oct;29(10):505-15
pubmed: 24028889
Malar J. 2010 Jan 27;9:34
pubmed: 20105282
Trop Med Int Health. 2009 Sep;14(9):1093-6
pubmed: 19624477
J Infect Dis. 2014 Jan 1;209(1):130-9
pubmed: 23922363
Malar J. 2012 Apr 12;11:113
pubmed: 22498364
J Infect Dis. 2002 Feb 1;185(3):380-8
pubmed: 11807721
J Infect Dis. 2003 Jun 15;187(12):1870-5
pubmed: 12792863
Pharmacol Rev. 2005 Mar;57(1):117-45
pubmed: 15734729
Am J Trop Med Hyg. 1995 Jun;52(6):565-8
pubmed: 7611566
BMJ Glob Health. 2018 Oct 19;3(5):e000999
pubmed: 30397515
Malar J. 2020 May 8;19(1):178
pubmed: 32384930
Drugs R D. 2019 Mar;19(1):1-14
pubmed: 30656608
Nature. 2000 Feb 24;403(6772):906-9
pubmed: 10706290
Malar Res Treat. 2018 May 2;2018:7071383
pubmed: 29854394
Malar J. 2014 Aug 06;13:300
pubmed: 25098280
Trans R Soc Trop Med Hyg. 2009 Apr;103 Suppl 1:S11-4
pubmed: 19084883
BMJ Glob Health. 2020 Sep;5(9):
pubmed: 32938611
J Infect Dis. 2013 Mar 1;207(5):842-7
pubmed: 23225895
Malar J. 2016 Dec 5;15(1):584
pubmed: 27919256
Am J Trop Med Hyg. 2006 Sep;75(3):388-95
pubmed: 16968911
Am J Trop Med Hyg. 2014 Oct;91(4):833-843
pubmed: 25048375
J Infect Dis. 2014 Aug 1;210(3):335-7
pubmed: 24610873
Malar J. 2017 Mar 27;16(1):130
pubmed: 28347314
Malar J. 2015 Aug 22;14:328
pubmed: 26296743
Nature. 1990 May 17;345(6272):255-8
pubmed: 2185424
PLoS One. 2016 Mar 31;11(3):e0151565
pubmed: 27031231
Antimicrob Agents Chemother. 2009 Aug;53(8):3405-10
pubmed: 19433569
Emerg Infect Dis. 2014 Jul;20(7):1140-8
pubmed: 24960247
Malar J. 2015 Nov 05;14:439
pubmed: 26542942
Malar J. 2016 Sep 05;15:452
pubmed: 27596849
J Antimicrob Chemother. 2015 Sep;70(9):2566-71
pubmed: 26080363
Am J Trop Med Hyg. 2006 Sep;75(3):396-401
pubmed: 16968912
Malar J. 2021 Jan 9;20(1):32
pubmed: 33422080
Pan Afr Med J. 2015 Jun 09;21:101
pubmed: 26516402