Functional, proteomic and phenotypic in vitro studies evidence podocyte injury after chronic exposure to heparin.
Cytoskeleton
Glomerular filtration
Heparin
Podocyte
Proteome
Journal
Toxicology and applied pharmacology
ISSN: 1096-0333
Titre abrégé: Toxicol Appl Pharmacol
Pays: United States
ID NLM: 0416575
Informations de publication
Date de publication:
15 10 2021
15 10 2021
Historique:
received:
19
05
2021
revised:
12
08
2021
accepted:
14
08
2021
pubmed:
20
8
2021
medline:
4
1
2022
entrez:
19
8
2021
Statut:
ppublish
Résumé
Unfractionated heparin (UFH) is a widely used anticoagulant that possess numerous properties including anti-inflammatory, anti-viral, anti-angiogenesis, and anti-metastatic effects. The effect of this drug was evaluated on the podocyte, an important actor of the glomerular filtration. Using a functional approach, we demonstrate that heparin treatment leads to a functional podocyte perturbation characterized by the increase of podocyte monolayer permeability. This effect is enhanced with time of exposure. Proteomic study reveals that heparin down regulate focal adhesion and cytoskeletal protein expressions as well as the synthesis of glomerular basement membrane components. This study clearly demonstrates that UFH may affect podocyte function by altering cytoskeleton organization, cell-cell contacts and cell attachment.
Identifiants
pubmed: 34411582
pii: S0041-008X(21)00287-8
doi: 10.1016/j.taap.2021.115683
pii:
doi:
Substances chimiques
Anticoagulants
0
Proteome
0
Heparin
9005-49-6
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
115683Informations de copyright
Copyright © 2021 Elsevier Inc. All rights reserved.