Cis regulation within a cluster of viral microRNAs.
Journal
Nucleic acids research
ISSN: 1362-4962
Titre abrégé: Nucleic Acids Res
Pays: England
ID NLM: 0411011
Informations de publication
Date de publication:
27 09 2021
27 09 2021
Historique:
accepted:
10
08
2021
revised:
06
08
2021
received:
19
11
2020
pubmed:
22
8
2021
medline:
21
12
2021
entrez:
21
8
2021
Statut:
ppublish
Résumé
MicroRNAs (miRNAs) are small regulatory RNAs involved in virtually all biological processes. Although many of them are co-expressed from clusters, little is known regarding the impact of this organization on the regulation of their accumulation. In this study, we set to decipher a regulatory mechanism controlling the expression of the ten clustered pre-miRNAs from Kaposi's sarcoma associated herpesvirus (KSHV). We measured in vitro the efficiency of cleavage of each individual pre-miRNA by the Microprocessor and found that pre-miR-K1 and -K3 were the most efficiently cleaved pre-miRNAs. A mutational analysis showed that, in addition to producing mature miRNAs, they are also important for the optimal expression of the whole set of miRNAs. We showed that this feature depends on the presence of a canonical pre-miRNA at this location since we could functionally replace pre-miR-K1 by a heterologous pre-miRNA. Further in vitro processing analysis suggests that the two stem-loops act in cis and that the cluster is cleaved in a sequential manner. Finally, we exploited this characteristic of the cluster to inhibit the expression of the whole set of miRNAs by targeting the pre-miR-K1 with LNA-based antisense oligonucleotides in cells either expressing a synthetic construct or latently infected with KSHV.
Identifiants
pubmed: 34417603
pii: 6355881
doi: 10.1093/nar/gkab731
pmc: PMC8464075
doi:
Substances chimiques
MicroRNAs
0
Oligonucleotides, Antisense
0
RNA, Viral
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
10018-10033Commentaires et corrections
Type : ErratumIn
Informations de copyright
© The Author(s) 2021. Published by Oxford University Press on behalf of Nucleic Acids Research.
Références
Nucleic Acids Res. 2021 May 7;49(8):4456-4471
pubmed: 33823555
Nat Cell Biol. 2010 Feb;12(2):193-9
pubmed: 20081837
J Virol. 2006 Mar;80(5):2234-42
pubmed: 16474131
Nucleic Acids Res. 2005 May 12;33(8):2697-706
pubmed: 15891114
RNA Biol. 2011 Nov-Dec;8(6):1105-14
pubmed: 21955497
J Virol. 2017 Jan 18;91(3):
pubmed: 27852859
Cell. 2007 Jul 13;130(1):89-100
pubmed: 17599402
J Virol. 2010 Mar;84(6):2697-706
pubmed: 20071580
Viruses. 2012 Sep;4(9):1687-710
pubmed: 23170179
Nucleic Acids Res. 2014 Apr;42(8):5234-44
pubmed: 24520115
RNA. 2012 May;18(5):1014-28
pubmed: 22450760
Nat Genet. 2015 Jul;47(7):766-75
pubmed: 26029871
Biol Rev Camb Philos Soc. 2018 Nov;93(4):1955-1986
pubmed: 29797774
J Virol. 2005 Jul;79(14):9301-5
pubmed: 15994824
Front Microbiol. 2012 May 03;3:165
pubmed: 22563327
J Virol. 2007 Dec;81(23):12836-45
pubmed: 17881434
Virology. 2004 Aug 1;325(2):225-40
pubmed: 15246263
Nucleic Acids Res. 2014 Jul;42(12):7981-96
pubmed: 24831544
Trends Microbiol. 2017 Aug;25(8):648-661
pubmed: 28259385
Nat Rev Mol Cell Biol. 2019 Jan;20(1):5-20
pubmed: 30228348
PLoS Biol. 2016 Nov 28;14(11):e2000998
pubmed: 27893764
Immunol Rev. 2013 May;253(1):158-66
pubmed: 23550645
Nucleic Acids Res. 2014 Jan;42(1):609-21
pubmed: 24068553
Nucleic Acids Res. 2020 Nov 4;48(19):11097-11112
pubmed: 33035348
Mol Cell. 2019 Jul 25;75(2):340-356.e10
pubmed: 31253575
Nature. 2007 Jul 5;448(7149):83-6
pubmed: 17589500
Nat Methods. 2005 Apr;2(4):269-76
pubmed: 15782219
ACS Nano. 2020 Jan 28;14(1):476-487
pubmed: 31895530
J Virol. 2010 May;84(10):5229-37
pubmed: 20219912
Biochim Biophys Acta. 2011 Nov-Dec;1809(11-12):700-7
pubmed: 21683814
J Virol. 2021 Feb 10;:
pubmed: 33568509
RNA. 2019 Jan;25(1):1-16
pubmed: 30333195
Nature. 2010 Jun 3;465(7298):584-9
pubmed: 20424607
PLoS Pathog. 2011 Dec;7(12):e1002405
pubmed: 22174674
J Virol Methods. 2011 Jun;174(1-2):12-21
pubmed: 21419799
PLoS Pathog. 2013;9(12):e1003857
pubmed: 24385912
Curr Opin Microbiol. 2010 Aug;13(4):540-5
pubmed: 20580307
Sci Rep. 2017 Jan 19;7:40813
pubmed: 28102325
Mol Cell. 2020 Apr 16;78(2):303-316.e4
pubmed: 32302542
RNA. 2016 Jan;22(1):129-38
pubmed: 26554028
Biol Chem. 2017 Jul 26;398(8):911-918
pubmed: 28284028
Science. 2010 Jun 25;328(5986):1694-8
pubmed: 20448148
PLoS Pathog. 2013;9(9):e1003584
pubmed: 24039573
Nucleic Acids Res. 2009 Jun;37(10):3464-73
pubmed: 19339516
Nature. 2007 Dec 13;450(7172):1096-9
pubmed: 18075594
Cell. 2018 Mar 22;173(1):20-51
pubmed: 29570994
Genome Res. 2004 Sep;14(9):1741-8
pubmed: 15310658
Nucleic Acids Res. 2016 Feb 18;44(3):1326-41
pubmed: 26635399
Viruses. 2016 Feb 19;8(2):
pubmed: 26907327
Front Microbiol. 2017 Apr 20;8:613
pubmed: 28473805
Methods Mol Biol. 2018;1823:141-152
pubmed: 29959679
Mol Cell. 2020 Jun 4;78(5):876-889.e6
pubmed: 32502422
Science. 2010 Jan 8;327(5962):198-201
pubmed: 19965718
Mol Cell. 2015 Oct 1;60(1):131-45
pubmed: 26412306
Sci Rep. 2017 Aug 17;7(1):8585
pubmed: 28819307
Mol Cell. 2020 Dec 3;80(5):892-902.e4
pubmed: 33188727
Proc Natl Acad Sci U S A. 2005 Apr 12;102(15):5570-5
pubmed: 15800047
PLoS One. 2011 Jan 20;6(1):e16224
pubmed: 21283761
Cell Host Microbe. 2009 Dec 17;6(6):570-5
pubmed: 20006845
Oncogene. 2017 Sep 21;36(38):5407-5420
pubmed: 28534512
Cell Rep. 2014 Oct 23;9(2):542-54
pubmed: 25310978
RNA. 2010 Aug;16(8):1540-58
pubmed: 20566670
Mol Cell. 2020 Apr 16;78(2):289-302.e6
pubmed: 32302541
FEBS Lett. 2018 Jun;592(12):1980-1996
pubmed: 29683487
Cell Host Microbe. 2009 Apr 23;5(4):376-85
pubmed: 19380116