Knox homologs shoot meristemless (STM) and KNAT6 are epistatic to CLAVATA3 (CLV3) during shoot meristem development in Arabidopsis thaliana.
Alleles
Arabidopsis
/ genetics
Arabidopsis Proteins
/ genetics
Gene Expression Regulation, Developmental
Gene Expression Regulation, Plant
Genes, Plant
Heterozygote
Homeodomain Proteins
/ genetics
Homozygote
Meristem
/ genetics
Mutation
Phenotype
Plants, Genetically Modified
Transcription Factors
/ genetics
CLV3
KNAT6
KNOX
Organ boundary specification
STM
Shoot apical meristem
Journal
Molecular biology reports
ISSN: 1573-4978
Titre abrégé: Mol Biol Rep
Pays: Netherlands
ID NLM: 0403234
Informations de publication
Date de publication:
Sep 2021
Sep 2021
Historique:
received:
05
05
2021
accepted:
03
08
2021
pubmed:
22
8
2021
medline:
29
1
2022
entrez:
21
8
2021
Statut:
ppublish
Résumé
In Arabidopsis, the genes SHOOT MERISTEMLESS (STM) and CLAVATA3 (CLV3) antagonistically regulate shoot meristem development. STM is essential for both development and maintenance of the meristem, as stm mutants fail to develop a shoot meristem. CLV3, on the other hand, negatively regulates meristem proliferation, and clv3 mutants possess an enlarged shoot meristem. Genetic interaction studies revealed that stm and clv3 dominantly suppress each other's phenotypes. STM works in conjunction with its closely related homologue KNOTTED1-LIKE HOMEOBOX GENE 6 (KNAT6) to promote meristem development and organ separation, as stm knat6 double mutants fail to form shoot meristem and produce a fused cotyledon. In this study, we show that clv3 fails to promote shoot meristem formation in stm-1 background if we also remove KNAT6. stm-1 knat6 clv3 triple mutants result in shoot meristem termination and produce fused cotyledons similar to stm knat6 double mutant. Notably, the stm-1 knat6 and stm-1 knat6 clv3 alleles lack tissue in the presumed region of SAM that is a novel phenotype reported in Arabidopsis mutants. stm-1 knat6 clv3 also showed reduced inflorescence size as compared to clv3 single or stm clv3 double mutants. In contrast to previously published data, these data suggest that STM and KNAT6 are redundantly required for the vegetative SAM, but insufficient for the inflorescence meristem.
Sections du résumé
BACKGROUND
BACKGROUND
In Arabidopsis, the genes SHOOT MERISTEMLESS (STM) and CLAVATA3 (CLV3) antagonistically regulate shoot meristem development. STM is essential for both development and maintenance of the meristem, as stm mutants fail to develop a shoot meristem. CLV3, on the other hand, negatively regulates meristem proliferation, and clv3 mutants possess an enlarged shoot meristem. Genetic interaction studies revealed that stm and clv3 dominantly suppress each other's phenotypes. STM works in conjunction with its closely related homologue KNOTTED1-LIKE HOMEOBOX GENE 6 (KNAT6) to promote meristem development and organ separation, as stm knat6 double mutants fail to form shoot meristem and produce a fused cotyledon.
RESULTS
RESULTS
In this study, we show that clv3 fails to promote shoot meristem formation in stm-1 background if we also remove KNAT6. stm-1 knat6 clv3 triple mutants result in shoot meristem termination and produce fused cotyledons similar to stm knat6 double mutant. Notably, the stm-1 knat6 and stm-1 knat6 clv3 alleles lack tissue in the presumed region of SAM that is a novel phenotype reported in Arabidopsis mutants. stm-1 knat6 clv3 also showed reduced inflorescence size as compared to clv3 single or stm clv3 double mutants.
CONCLUSION
CONCLUSIONS
In contrast to previously published data, these data suggest that STM and KNAT6 are redundantly required for the vegetative SAM, but insufficient for the inflorescence meristem.
Identifiants
pubmed: 34417947
doi: 10.1007/s11033-021-06622-4
pii: 10.1007/s11033-021-06622-4
doi:
Substances chimiques
AT2G27250 protein, Arabidopsis
0
Arabidopsis Proteins
0
Homeodomain Proteins
0
KNAT6 protein, Arabidopsis
0
STM protein, Arabidopsis
0
Transcription Factors
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
6291-6302Informations de copyright
© 2021. The Author(s), under exclusive licence to Springer Nature B.V.
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