Animal welfare and zoonosis risk: anti-Trichinella antibodies in breeding pigs farmed under controlled housing conditions.
Animal welfare
Breeding pig
Housing condition
Outdoor access
Serology
Trichinella
Journal
Parasites & vectors
ISSN: 1756-3305
Titre abrégé: Parasit Vectors
Pays: England
ID NLM: 101462774
Informations de publication
Date de publication:
21 Aug 2021
21 Aug 2021
Historique:
received:
03
05
2021
accepted:
02
08
2021
entrez:
22
8
2021
pubmed:
23
8
2021
medline:
15
12
2021
Statut:
epublish
Résumé
Domesticated pigs are the main source of Trichinella sp. infections for humans, particularly when reared in backyards or free-ranging. In temperate areas of southern Europe, most pigs are farmed under controlled housing conditions, but sows and sometimes fattening pigs have access to outdoors to improve animal welfare. The aim of the present study was to investigate whether outdoor access of breeding pigs farmed under controlled housing conditions can represent a risk for Trichinella sp. transmission when the farm is located in an agricultural area interspersed with wooded areas and badlands, where Trichinella spp. could be present in wildlife. Serum samples were collected from 63 breeding sows and one boar before and after their access to an open fenced area for 2 months and from 84 pigs that never had outdoor access. Samples were screened for anti-Trichinella antibodies by ELISA, and positive sera were confirmed using Western blot (Wb) excretory/secretory antigens. To detect Trichinella sp. larvae, muscle tissues from serologically positive and negative pigs were tested by artificial digestion. Thirteen (20.6%) sows and one boar tested positive with both ELISA and Wb. No larvae were detected in muscle samples of serologically positive and serologically negative pigs. Positive serum samples were then tested by Wb using crude worm extract as antigens. The Wb banding pattern displayed was that characteristic of encapsulated species (Trichinella spiralis or Trichinella britovi). The detection of anti-Trichinella antibodies without larvae in the pig muscles, supported by epidemiological data, suggests that pigs may have been exposed to T. britovi. This study stresses the importance of instigating monitoring systems at farm level to prevent Trichinella sp. transmission and to investigate, through a landscape parasitological study, the suitability of a site before the planting of a high containment level pig farm in which the sows can have outside access to improve their welfare during pregnancy.
Sections du résumé
BACKGROUND
BACKGROUND
Domesticated pigs are the main source of Trichinella sp. infections for humans, particularly when reared in backyards or free-ranging. In temperate areas of southern Europe, most pigs are farmed under controlled housing conditions, but sows and sometimes fattening pigs have access to outdoors to improve animal welfare. The aim of the present study was to investigate whether outdoor access of breeding pigs farmed under controlled housing conditions can represent a risk for Trichinella sp. transmission when the farm is located in an agricultural area interspersed with wooded areas and badlands, where Trichinella spp. could be present in wildlife.
METHODS
METHODS
Serum samples were collected from 63 breeding sows and one boar before and after their access to an open fenced area for 2 months and from 84 pigs that never had outdoor access. Samples were screened for anti-Trichinella antibodies by ELISA, and positive sera were confirmed using Western blot (Wb) excretory/secretory antigens. To detect Trichinella sp. larvae, muscle tissues from serologically positive and negative pigs were tested by artificial digestion.
RESULTS
RESULTS
Thirteen (20.6%) sows and one boar tested positive with both ELISA and Wb. No larvae were detected in muscle samples of serologically positive and serologically negative pigs. Positive serum samples were then tested by Wb using crude worm extract as antigens. The Wb banding pattern displayed was that characteristic of encapsulated species (Trichinella spiralis or Trichinella britovi).
CONCLUSIONS
CONCLUSIONS
The detection of anti-Trichinella antibodies without larvae in the pig muscles, supported by epidemiological data, suggests that pigs may have been exposed to T. britovi. This study stresses the importance of instigating monitoring systems at farm level to prevent Trichinella sp. transmission and to investigate, through a landscape parasitological study, the suitability of a site before the planting of a high containment level pig farm in which the sows can have outside access to improve their welfare during pregnancy.
Identifiants
pubmed: 34419112
doi: 10.1186/s13071-021-04920-1
pii: 10.1186/s13071-021-04920-1
pmc: PMC8379739
doi:
Substances chimiques
Antibodies, Helminth
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
417Subventions
Organisme : Directorate-General for Health and Food Safety
ID : SI2.801980
Informations de copyright
© 2021. The Author(s).
Références
Parasite. 2001 Jun;8(2 Suppl):S216-22
pubmed: 11484361
Int J Parasitol Parasites Wildl. 2017 Jan 18;6(1):1-7
pubmed: 28180084
Vet Parasitol. 2017 Nov 15;246:1-4
pubmed: 28969769
J Parasitol. 1992 Aug;78(4):654-9
pubmed: 1635024
Vet Parasitol. 2005 Sep 5;132(1-2):85-90
pubmed: 15985334
Parasit Vectors. 2016 Mar 16;9:154
pubmed: 26984402
Zoonoses Public Health. 2021 Feb;68(1):29-37
pubmed: 33164335
Pest Manag Sci. 2015 Apr;71(4):492-500
pubmed: 25512181
Vet Parasitol. 2011 Jun 10;178(3-4):370-3
pubmed: 21296503
Vet Parasitol. 2009 Dec 23;166(3-4):241-8
pubmed: 19819075
Int J Parasitol. 2000 Feb;30(2):215-21
pubmed: 10704604
Vet Parasitol. 2016 Nov 15;231:124-127
pubmed: 27522469
Parasit Vectors. 2020 Oct 16;13(1):520
pubmed: 33066824
Parasit Vectors. 2018 Dec 12;11(1):631
pubmed: 30541617
Zoonoses Public Health. 2015 Jun;62(4):285-91
pubmed: 25103623
Parasite. 2019;26:42
pubmed: 31309926
Trends Parasitol. 2014 Jan;30(1):4-11
pubmed: 24314577
Int J Parasitol. 2012 Oct;42(11):1017-23
pubmed: 22995743