The Heparan Sulfate Binding Peptide in Tumor Progression of Triple-Negative Breast Cancer.

anti-angiogenic breast cancer (BC) breast neoplasia glycosaminoglycans phage display

Journal

Frontiers in oncology
ISSN: 2234-943X
Titre abrégé: Front Oncol
Pays: Switzerland
ID NLM: 101568867

Informations de publication

Date de publication:
2021
Historique:
received: 20 04 2021
accepted: 29 06 2021
entrez: 23 8 2021
pubmed: 24 8 2021
medline: 24 8 2021
Statut: epublish

Résumé

Angiogenesis is the formation of new vessels from pre-existing vasculature. The heparan sulfate chains from endothelial cell proteoglycans interact with the major angiogenic factors, regulating blood vessels´ formation. Since the FDA´s first approval, anti-angiogenic therapy has shown tumor progression inhibition and increased patient survival. Previous work in our group has selected an HS-binding peptide using a phage display system. Therefore, we investigated the effect of the selected peptide in angiogenesis and tumor progression. The HS-binding peptide showed a higher affinity for heparin N-sulfated. The HS-binding peptide was able to inhibit the proliferation of human endothelial umbilical cord cells (HUVEC) by modulation of FGF-2. It was verified a significant decrease in the tube formation of human endothelial cells and capillary formation of mice aorta treated with HS-binding peptide. HS-binding peptide also inhibited the formation of sub-intestinal blood vessels in zebrafish embryos. Additionally, in zebrafish embryos, the tumor size decreased after treatment with HS-binding peptide.

Identifiants

pubmed: 34422650
doi: 10.3389/fonc.2021.697626
pmc: PMC8372403
doi:

Types de publication

Journal Article

Langues

eng

Pagination

697626

Informations de copyright

Copyright © 2021 Melo, Wang, Fujimura, Strnadel, Meneghetti, Nader, Klemke and Pinhal.

Déclaration de conflit d'intérêts

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

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Auteurs

Carina Mucciolo Melo (CM)

Department of Biochemistry/Molecular Biology, Universidade Federal de São Paulo, São Paulo, Brazil.
Department of Biochemistry, Faculdade de Medicina do ABC, Santo André, Brazil.

Huawei Wang (H)

Department of Pathology, School of Medicine, University of California San Diego, La Jolla, CA, United States.

Ken Fujimura (K)

Department of Pathology, School of Medicine, University of California San Diego, La Jolla, CA, United States.

Jan Strnadel (J)

Department of Molecular Medicine, Biomedical Center Martin, Jessenius Faculty of Medicine in Martin, Comenius University in Bratislava, Martin, Slovakia.

Maria Cecília Zorél Meneghetti (MCZ)

Department of Biochemistry/Molecular Biology, Universidade Federal de São Paulo, São Paulo, Brazil.

Helena Bonciani Nader (HB)

Department of Biochemistry/Molecular Biology, Universidade Federal de São Paulo, São Paulo, Brazil.

Richard L Klemke (RL)

Department of Pathology, School of Medicine, University of California San Diego, La Jolla, CA, United States.

Maria Aparecida Silva Pinhal (MAS)

Department of Biochemistry/Molecular Biology, Universidade Federal de São Paulo, São Paulo, Brazil.
Department of Biochemistry, Faculdade de Medicina do ABC, Santo André, Brazil.

Classifications MeSH