Integrated single-cell analysis unveils diverging immune features of COVID-19, influenza, and other community-acquired pneumonia.
COVID-19
PBMC
Pneumonia
human
immunology
infectious disease
inflammation
microbiology
Journal
eLife
ISSN: 2050-084X
Titre abrégé: Elife
Pays: England
ID NLM: 101579614
Informations de publication
Date de publication:
23 08 2021
23 08 2021
Historique:
received:
23
04
2021
accepted:
13
08
2021
entrez:
23
8
2021
pubmed:
24
8
2021
medline:
3
9
2021
Statut:
epublish
Résumé
The exact immunopathophysiology of community-acquired pneumonia (CAP) caused by SARS-CoV-2 (COVID-19) remains clouded by a general lack of relevant disease controls. The scarcity of single-cell investigations in the broader population of patients with CAP renders it difficult to distinguish immune features unique to COVID-19 from the common characteristics of a dysregulated host response to pneumonia. We performed integrated single-cell transcriptomic and proteomic analyses in peripheral blood mononuclear cells from a matched cohort of eight patients with COVID-19, eight patients with CAP caused by Influenza A or other pathogens, and four non-infectious control subjects. Using this balanced, multi-omics approach, we describe shared and diverging transcriptional and phenotypic patterns-including increased levels of type I interferon-stimulated natural killer cells in COVID-19, cytotoxic CD8 T EMRA cells in both COVID-19 and influenza, and distinctive monocyte compositions between all groups-and thereby expand our understanding of the peripheral immune response in different etiologies of pneumonia.
Identifiants
pubmed: 34424199
doi: 10.7554/eLife.69661
pii: 69661
pmc: PMC8382293
doi:
pii:
Banques de données
GEO
['GSE164948']
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : NWO
ID : 16447
Organisme : European Commission
ID : 847786
Organisme : AMC
ID : Corona Research Fund
Informations de copyright
© 2021, Schuurman et al.
Déclaration de conflit d'intérêts
AS, TR, AS, IR, MS, Jd, Cv, LV, BS, WW, FV, Tv No competing interests declared
Références
Immunology. 2020 Apr;159(4):365-372
pubmed: 31792954
Nature. 2012 Mar 25;484(7395):519-23
pubmed: 22446628
JAMA Intern Med. 2020 Oct 1;180(10):1336-1344
pubmed: 32609310
Thorax. 2012 Jan;67(1):71-9
pubmed: 20729232
Sci Immunol. 2020 Jun 26;5(48):
pubmed: 32591408
Nat Rev Microbiol. 2011 Oct 17;9(12):860-75
pubmed: 22002165
Proc Natl Acad Sci U S A. 2019 Jun 4;116(23):11370-11379
pubmed: 31113877
Nat Rev Nephrol. 2018 Feb;14(2):121-137
pubmed: 29225343
Ann N Y Acad Sci. 2020 Mar 23;:
pubmed: 32202669
Nat Med. 2020 Oct;26(10):1623-1635
pubmed: 32807934
Lancet. 2020 Feb 15;395(10223):497-506
pubmed: 31986264
Cell. 2019 Jun 13;177(7):1888-1902.e21
pubmed: 31178118
Cell. 2020 Sep 17;182(6):1419-1440.e23
pubmed: 32810438
Radiology. 2021 Jan;298(1):E30-E37
pubmed: 32776832
Cell Host Microbe. 2020 Jun 10;27(6):992-1000.e3
pubmed: 32320677
N Engl J Med. 2015 Jul 30;373(5):415-27
pubmed: 26172429
JAMA. 2020 Aug 25;324(8):782-793
pubmed: 32648899
PLoS One. 2012;7(2):e31535
pubmed: 22363665
QJM. 2001 Oct;94(10):521-6
pubmed: 11588210
Nat Rev Immunol. 2013 Dec;13(12):862-74
pubmed: 24232462
Infect Immun. 2019 Dec 17;88(1):
pubmed: 31611276
Emerg Infect Dis. 2008 Aug;14(8):1187-92
pubmed: 18680640
Nat Med. 2021 May;27(5):904-916
pubmed: 33879890
Nat Med. 2020 Jun;26(6):842-844
pubmed: 32398875
Sci Immunol. 2020 Jul 10;5(49):
pubmed: 32651212
Immunity. 2020 Sep 15;53(3):685-696.e3
pubmed: 32783921
JAMA. 2020 May 26;323(20):2052-2059
pubmed: 32320003
Nat Methods. 2017 Sep;14(9):865-868
pubmed: 28759029
PLoS One. 2017 May 8;12(5):e0177405
pubmed: 28481945
Nat Commun. 2015 Apr 21;6:6881
pubmed: 25898173
Virus Res. 2011 Dec;162(1-2):12-8
pubmed: 22027189
Nat Commun. 2020 Jul 6;11(1):3434
pubmed: 32632085
Front Immunol. 2018 Mar 05;9:320
pubmed: 29556226
Am J Respir Crit Care Med. 2017 Apr 1;195(7):906-911
pubmed: 27649072
Cell. 2021 Apr 1;184(7):1895-1913.e19
pubmed: 33657410
Nat Rev Immunol. 2020 Sep;20(9):529-536
pubmed: 32728222
Nat Rev Immunol. 2017 Jul;17(7):407-420
pubmed: 28436424
PLoS One. 2011;6(9):e25060
pubmed: 21966414
J Virol. 2012 Dec;86(24):13445-55
pubmed: 23015724
PLoS One. 2012;7(1):e29443
pubmed: 22238612
Front Immunol. 2018 Nov 20;9:2692
pubmed: 30515169
Proc Natl Acad Sci U S A. 2020 Mar 24;117(12):6686-6696
pubmed: 32161126
Science. 2020 Sep 4;369(6508):
pubmed: 32669297
Front Immunol. 2018 Apr 09;9:678
pubmed: 29686673
Nat Rev Immunol. 2020 Jun;20(6):355-362
pubmed: 32376901
Sci Signal. 2019 Oct 29;12(605):
pubmed: 31662487
Respir Res. 2019 May 2;20(1):82
pubmed: 31046764
Immunity. 2015 Feb 17;42(2):216-226
pubmed: 25692699
Nat Med. 2020 Jul;26(7):1070-1076
pubmed: 32514174
Sci Immunol. 2020 Jul 15;5(49):
pubmed: 32669287
Front Immunol. 2020 Jun 23;11:1512
pubmed: 32655581
Cell Rep Med. 2020 Sep 22;1(6):100081
pubmed: 32839763
Front Immunol. 2018 Jul 30;9:1726
pubmed: 30105020
Nat Immunol. 2020 Sep;21(9):1107-1118
pubmed: 32788748