RanGTP and the actin cytoskeleton keep paternal and maternal chromosomes apart during fertilization.
Journal
The Journal of cell biology
ISSN: 1540-8140
Titre abrégé: J Cell Biol
Pays: United States
ID NLM: 0375356
Informations de publication
Date de publication:
04 10 2021
04 10 2021
Historique:
received:
01
12
2020
revised:
18
06
2021
accepted:
06
08
2021
entrez:
23
8
2021
pubmed:
24
8
2021
medline:
1
12
2021
Statut:
ppublish
Résumé
Zygotes require two accurate sets of parental chromosomes, one each from the mother and the father, to undergo normal embryogenesis. However, upon egg-sperm fusion in vertebrates, the zygote has three sets of chromosomes, one from the sperm and two from the egg. The zygote therefore eliminates one set of maternal chromosomes (but not the paternal chromosomes) into the polar body through meiosis, but how the paternal chromosomes are protected from maternal meiosis has been unclear. Here we report that RanGTP and F-actin dynamics prevent egg-sperm fusion in proximity to maternal chromosomes. RanGTP prevents the localization of Juno and CD9, egg membrane proteins that mediate sperm fusion, at the cell surface in proximity to maternal chromosomes. Following egg-sperm fusion, F-actin keeps paternal chromosomes away from maternal chromosomes. Disruption of these mechanisms causes the elimination of paternal chromosomes during maternal meiosis. This study reveals a novel critical mechanism that prevents aneuploidy in zygotes.
Identifiants
pubmed: 34424312
pii: 212591
doi: 10.1083/jcb.202012001
pmc: PMC8404465
pii:
doi:
Substances chimiques
ran GTP-Binding Protein
EC 3.6.5.2
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Bill & Melinda Gates Foundation
ID : INV-001902
Pays : United States
Commentaires et corrections
Type : CommentIn
Informations de copyright
© 2021 Mori et al.
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