Elevated Expression of MiR-17 in Microglia of Alzheimer's Disease Patients Abrogates Autophagy-Mediated Amyloid-β Degradation.


Journal

Frontiers in immunology
ISSN: 1664-3224
Titre abrégé: Front Immunol
Pays: Switzerland
ID NLM: 101560960

Informations de publication

Date de publication:
2021
Historique:
received: 05 05 2021
accepted: 30 06 2021
entrez: 24 8 2021
pubmed: 25 8 2021
medline: 21 12 2021
Statut: epublish

Résumé

Autophagy is a proposed route of amyloid-β (Aβ) clearance by microglia that is halted in Alzheimer's Disease (AD), though mechanisms underlying this dysfunction remain elusive. Here, primary microglia from adult AD (5xFAD) mice were utilized to demonstrate that 5xFAD microglia fail to degrade Aβ and express low levels of autophagy cargo receptor NBR1. In 5xFAD mouse brains, we show for the first time that AD microglia express elevated levels of microRNA cluster Mirc1/Mir17-92a, which is known to downregulate autophagy proteins. By

Identifiants

pubmed: 34426734
doi: 10.3389/fimmu.2021.705581
pmc: PMC8379081
doi:

Substances chimiques

Amyloid beta-Peptides 0
MIRN17 microRNA, human 0
MicroRNAs 0
Mirn17 microRNA, mouse 0

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

705581

Subventions

Organisme : NIA NIH HHS
ID : R21 AG067755
Pays : United States
Organisme : NIA NIH HHS
ID : RF1 AG028271
Pays : United States
Organisme : NHLBI NIH HHS
ID : R01 HL127651
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI124121
Pays : United States
Organisme : NIA NIH HHS
ID : R21 AG064899
Pays : United States
Organisme : NCI NIH HHS
ID : P30 CA016058
Pays : United States

Informations de copyright

Copyright © 2021 Estfanous, Daily, Eltobgy, Deems, Anne, Krause, Badr, Hamilton, Carafice, Hegazi, Abu Khweek, Kelani, Nimjee, Awad, Zhang, Cormet-Boyaka, Haffez, Soror, Mikhail, Nuovo, Barrientos, Gavrilin and Amer.

Déclaration de conflit d'intérêts

AM and GN were employed by Gnome Diagnostic. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Références

Autophagy. 2014 Oct 1;10(10):1761-75
pubmed: 25126727
Cell. 2019 Jul 25;178(3):536-551.e14
pubmed: 31257024
Int J Mol Sci. 2021 Mar 24;22(7):
pubmed: 33805142
J Cyst Fibros. 2018 Jul;17(4):454-461
pubmed: 29241629
J Neurosci. 2014 Oct 22;34(43):14484-501
pubmed: 25339759
PLoS One. 2015 May 20;10(5):e0126423
pubmed: 25992776
Proc Natl Acad Sci U S A. 2006 Jan 3;103(1):141-6
pubmed: 16373510
Front Aging Neurosci. 2018 Jan 30;10:04
pubmed: 29441009
Cell. 2013 Sep 12;154(6):1257-68
pubmed: 24034249
J Neural Transm (Vienna). 2010 Aug;117(8):949-60
pubmed: 20552234
Cancer Biol Ther. 2013 Jul;14(7):574-86
pubmed: 23792642
Nucleic Acids Res. 2018 Jan 4;46(D1):D239-D245
pubmed: 29156006
J Alzheimers Dis. 2016;51(3):915-27
pubmed: 26923027
Ann Diagn Pathol. 2017 Jun;28:24-29
pubmed: 28648936
J Biol Chem. 2010 Nov 26;285(48):37405-14
pubmed: 20876579
Cell Rep. 2018 Sep 4;24(10):2773-2783.e6
pubmed: 30184509
Neuroreport. 2007 Feb 12;18(3):297-300
pubmed: 17314675
Brain Pathol. 2021 Jan;31(1):84-102
pubmed: 32654284
EMBO J. 2020 Dec 15;39(24):e104948
pubmed: 33226137
Cell Cycle. 2009 Jul 1;8(13):1986-90
pubmed: 19502794
J Clin Neurosci. 2006 Jun;13(5):563-8
pubmed: 16540327
STAR Protoc. 2020 Jun 19;1(1):
pubmed: 32783030
Autophagy. 2007 Nov-Dec;3(6):581-5
pubmed: 17700057
Carcinogenesis. 2012 Nov;33(11):2018-25
pubmed: 22902544
EMBO J. 2019 Feb 15;38(4):
pubmed: 30617086
Adv Sci (Weinh). 2019 Nov 22;7(2):1901555
pubmed: 31993283
Mol Neurodegener. 2011 Oct 07;6:70
pubmed: 21982160
Gerontology. 2017;63(1):20-28
pubmed: 27577994
J Vis Exp. 2012 Jul 30;(65):
pubmed: 22871843
Geroscience. 2020 Apr;42(2):613-632
pubmed: 31975051
Nat Commun. 2018 Oct 1;9(1):4011
pubmed: 30275444
Nat Neurosci. 2020 Oct;23(10):1183-1193
pubmed: 32778792
Autophagy. 2016;12(1):1-222
pubmed: 26799652
J Neurosci. 2008 Aug 13;28(33):8354-60
pubmed: 18701698
EMBO J. 2017 Sep 1;36(17):2473-2487
pubmed: 28768718
Proc Natl Acad Sci U S A. 1994 Dec 6;91(25):12243-7
pubmed: 7991613
Curr Genomics. 2009 May;10(3):154-68
pubmed: 19881909
Am J Pathol. 2005 May;166(5):1475-85
pubmed: 15855647
Ann Transl Med. 2018 May;6(10):176
pubmed: 29951498
PLoS One. 2012;7(11):e50837
pubmed: 23226399
J Neurosci. 2006 Oct 4;26(40):10129-40
pubmed: 17021169
Autophagy. 2021 Jan;17(1):1-382
pubmed: 33634751
Genomics. 2020 Mar;112(2):1290-1299
pubmed: 31377428
Brain Res. 2016 Oct 15;1649(Pt B):143-150
pubmed: 27038755
Mol Cell. 2014 Jun 19;54(6):1042-1054
pubmed: 24857550
J Neurosci. 2012 Oct 17;32(42):14641-8
pubmed: 23077050
Eur J Pharmacol. 2021 Mar 15;895:173873
pubmed: 33460611
Curr Opin Microbiol. 2013 Jun;16(3):339-48
pubmed: 23623150
Brain Behav Immun. 2014 Nov;42:22-32
pubmed: 24998196
Neuron. 2013 Sep 4;79(5):873-86
pubmed: 24012002
Curr Protoc Immunol. 2019 Jun;125(1):e70
pubmed: 30414379
Autophagy. 2016 Nov;12(11):2026-2037
pubmed: 27541364
Cell Mol Neurobiol. 2017 Apr;37(3):377-388
pubmed: 27260250
Cell Mol Life Sci. 2017 Jun;74(12):2167-2201
pubmed: 28197669

Auteurs

Shady Estfanous (S)

Department of Microbial Infection and Immunity, Infectious Diseases Institute, The Heart and Lung Research Institute, Ohio State University, Columbus, OH, United States.
Biochemistry and Molecular Biology Department, Faculty of Pharmacy, Helwan University, Cairo, Egypt.

Kylene P Daily (KP)

Department of Microbial Infection and Immunity, Infectious Diseases Institute, The Heart and Lung Research Institute, Ohio State University, Columbus, OH, United States.

Mostafa Eltobgy (M)

Department of Microbial Infection and Immunity, Infectious Diseases Institute, The Heart and Lung Research Institute, Ohio State University, Columbus, OH, United States.

Nicholas P Deems (NP)

Institute for Behavioral Medicine Research, Ohio State University, Columbus, OH, United States.

Midhun N K Anne (MNK)

Department of Microbial Infection and Immunity, Infectious Diseases Institute, The Heart and Lung Research Institute, Ohio State University, Columbus, OH, United States.

Kathrin Krause (K)

Department of Microbial Infection and Immunity, Infectious Diseases Institute, The Heart and Lung Research Institute, Ohio State University, Columbus, OH, United States.
Max Planck Unit for the Science of Pathogens, Berlin, Germany.

Asmaa Badr (A)

Department of Microbial Infection and Immunity, Infectious Diseases Institute, The Heart and Lung Research Institute, Ohio State University, Columbus, OH, United States.

Kaitlin Hamilton (K)

Department of Microbial Infection and Immunity, Infectious Diseases Institute, The Heart and Lung Research Institute, Ohio State University, Columbus, OH, United States.

Cierra Carafice (C)

Department of Microbial Infection and Immunity, Infectious Diseases Institute, The Heart and Lung Research Institute, Ohio State University, Columbus, OH, United States.

Ahmad Hegazi (A)

Department of Microbial Infection and Immunity, Infectious Diseases Institute, The Heart and Lung Research Institute, Ohio State University, Columbus, OH, United States.

Arwa Abu Khweek (A)

Department of Microbial Infection and Immunity, Infectious Diseases Institute, The Heart and Lung Research Institute, Ohio State University, Columbus, OH, United States.
Department of Biology and Biochemistry, Birzeit University, West Bank, Palestine.

Hesham Kelani (H)

Department of Anesthesiology, Wexner Medical Center, The Ohio State University, Columbus, OH, United States.

Shahid Nimjee (S)

Department of Neurological Surgery, Wexner Medical Center, The Ohio State University, Columbus, OH, United States.

Hamdy Awad (H)

Department of Anesthesiology, Wexner Medical Center, The Ohio State University, Columbus, OH, United States.

Xiaoli Zhang (X)

Center for Biostatistics, Ohio State University, Columbus, OH, United States.

Estelle Cormet-Boyaka (E)

Department of Veterinary Biosciences, The Ohio State University, Columbus, OH, United States.

Hesham Haffez (H)

Biochemistry and Molecular Biology Department, Faculty of Pharmacy, Helwan University, Cairo, Egypt.
Center of Excellence, Helwan Structure Biology Research, Cairo, Egypt.

Sameh Soror (S)

Biochemistry and Molecular Biology Department, Faculty of Pharmacy, Helwan University, Cairo, Egypt.
Center of Excellence, Helwan Structure Biology Research, Cairo, Egypt.

Adel Mikhail (A)

GNOME DIAGNOSTICS, Department of Scientific Research, Powell, OH, United States.

Gerard Nuovo (G)

GNOME DIAGNOSTICS, Department of Scientific Research, Powell, OH, United States.

Ruth M Barrientos (RM)

Institute for Behavioral Medicine Research, Ohio State University, Columbus, OH, United States.

Mikhail A Gavrilin (MA)

Department of Internal Medicine, Ohio State University, Columbus, OH, United States.

Amal O Amer (AO)

Department of Microbial Infection and Immunity, Infectious Diseases Institute, The Heart and Lung Research Institute, Ohio State University, Columbus, OH, United States.

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