Depletion of TAX1BP1 Amplifies Innate Immune Responses during Respiratory Syncytial Virus Infection.
Animals
Cell Line
Cricetinae
Humans
Immunity, Innate
Intracellular Signaling Peptides and Proteins
/ immunology
Mice
Mice, Knockout
Neoplasm Proteins
/ immunology
Nucleocapsid Proteins
/ immunology
Respiratory Syncytial Virus Infections
/ immunology
Respiratory Syncytial Virus, Human
/ immunology
Virus Replication
RSV
TAX1BP1
innate immunity
interferons
lung
nucleoprotein
yeast two-hybrid screening
Journal
Journal of virology
ISSN: 1098-5514
Titre abrégé: J Virol
Pays: United States
ID NLM: 0113724
Informations de publication
Date de publication:
27 10 2021
27 10 2021
Historique:
pubmed:
26
8
2021
medline:
25
12
2021
entrez:
25
8
2021
Statut:
ppublish
Résumé
Respiratory syncytial virus (RSV) is the main cause of acute respiratory infections in young children and also has a major impact on the elderly and immunocompromised people. In the absence of a vaccine or efficient treatment, a better understanding of RSV interactions with the host antiviral response during infection is needed. Previous studies revealed that cytoplasmic inclusion bodies (IBs), where viral replication and transcription occur, could play a major role in the control of innate immunity during infection by recruiting cellular proteins involved in the host antiviral response. We recently showed that the morphogenesis of IBs relies on a liquid-liquid-phase separation mechanism depending on the interaction between viral nucleoprotein (N) and phosphoprotein (P). These scaffold proteins are expected to play a central role in the recruitment of cellular proteins to IBs. Here, we performed a yeast two-hybrid screen using RSV N protein as bait and identified the cellular protein TAX1BP1 as a potential partner of this viral protein. This interaction was validated by pulldown and immunoprecipitation assays. We showed that TAX1BP1 suppression has only a limited impact on RSV infection in cell cultures. However, RSV replication is decreased in TAX1BP1-deficient (TAX1BP1 knockout [TAX1BP1
Identifiants
pubmed: 34431698
doi: 10.1128/JVI.00912-21
pmc: PMC8549506
doi:
Substances chimiques
Intracellular Signaling Peptides and Proteins
0
Neoplasm Proteins
0
Nucleocapsid Proteins
0
TAX1BP1 protein, human
0
TAX1BP1 protein, mouse
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0091221Subventions
Organisme : Agence Nationale de la Recherche (ANR)
ID : ANR-13-IVS3-0007
Organisme : Governo Brasil (Brazilian Government)
ID : RESPISYNCELL 2013/50299-2
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