The related coactivator complexes SAGA and ATAC control embryonic stem cell self-renewal through acetyltransferase-independent mechanisms.

Animals Cell Self Renewal / physiology Embryonic Stem Cells / metabolism Histone Acetyltransferases / metabolism Histones / metabolism Mice Protein Processing, Post-Translational / physiology RNA Polymerase II / metabolism Saccharomyces cerevisiae / metabolism Saccharomyces cerevisiae Proteins / metabolism Trans-Activators / metabolism
4sU labeling ATAC HAT-independent function Pol II transcription SAGA coactivator complexes histone acetyltransferase mouse embryonic stem cells newly synthesized RNA

Journal

Cell reports
ISSN: 2211-1247
Titre abrégé: Cell Rep
Pays: United States
ID NLM: 101573691

Informations de publication

Date de publication:
24 08 2021
Historique:
received: 27 01 2021
revised: 17 06 2021
accepted: 03 08 2021
entrez: 25 8 2021
pubmed: 26 8 2021
medline: 12 2 2022
Statut: ppublish

Résumé

SAGA (Spt-Ada-Gcn5 acetyltransferase) and ATAC (Ada-two-A-containing) are two related coactivator complexes, sharing the same histone acetyltransferase (HAT) subunit. The HAT activities of SAGA and ATAC are required for metazoan development, but the role of these complexes in RNA polymerase II transcription is less understood. To determine whether SAGA and ATAC have redundant or specific functions, we compare the effects of HAT inactivation in each complex with that of inactivation of either SAGA or ATAC core subunits in mouse embryonic stem cells (ESCs). We show that core subunits of SAGA or ATAC are required for complex assembly and mouse ESC growth and self-renewal. Surprisingly, depletion of HAT module subunits causes a global decrease in histone H3K9 acetylation, but does not result in significant phenotypic or transcriptional defects. Thus, our results indicate that SAGA and ATAC are differentially required for self-renewal of mouse ESCs by regulating transcription through different pathways in a HAT-independent manner.

Identifiants

DOI: 10.1016/j.celrep.2021.109598 PMID: 34433046 PMC: PMC8430043
pubmed: 34433046
pii: S2211-1247(21)01036-6
doi: 10.1016/j.celrep.2021.109598
pmc: PMC8430043
mid: NIHMS1735653
pii:
doi:

Substances chimiques

Histones 0
Saccharomyces cerevisiae Proteins 0
Trans-Activators 0
Histone Acetyltransferases EC 2.3.1.48
RNA Polymerase II EC 2.7.7.-

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

109598

Subventions

Organisme : NIGMS NIH HHS
ID : R01 GM131626
Pays : United States

Informations de copyright

Copyright © 2021 The Authors. Published by Elsevier Inc. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of interests The authors declare no competing interests.

Références

Bioinformatics. 2013 Jan 1;29(1):15-21
pubmed: 23104886
Cell. 2016 Feb 11;164(4):668-80
pubmed: 26871632
J Biol Chem. 2015 Nov 27;290(48):28997-9009
pubmed: 26468280
Cell. 2006 Jun 2;125(5):957-69
pubmed: 16751104
Epigenomics. 2014 Jun;6(3):329-39
pubmed: 25111486
Regen Med. 2010 Nov;5(6):947-59
pubmed: 21082893
Cell. 2007 Feb 23;128(4):707-19
pubmed: 17320508
Cell. 2007 Feb 23;128(4):693-705
pubmed: 17320507
Bioinformatics. 2015 Jan 15;31(2):166-9
pubmed: 25260700
Mol Cell. 2017 May 18;66(4):568-576.e4
pubmed: 28483418
Cell. 1994 Oct 7;79(1):107-17
pubmed: 7923369
Mol Cell Biol. 1995 Apr;15(4):1999-2009
pubmed: 7891695
Proc Natl Acad Sci U S A. 2000 Oct 10;97(21):11303-6
pubmed: 11027331
Annu Rev Cell Dev Biol. 2014;30:647-75
pubmed: 25288119
Nature. 2016 Jan 28;529(7587):490-5
pubmed: 26735016
J Biol Chem. 2008 Dec 5;283(49):33808-15
pubmed: 18838386
Proc Natl Acad Sci U S A. 2015 Oct 6;112(40):E5461-70
pubmed: 26401015
J Mol Biol. 2017 May 19;429(10):1476-1503
pubmed: 27988225
Nucleic Acids Res. 2002 Jan 1;30(1):207-10
pubmed: 11752295
Mol Cell Biol. 2002 Aug;22(15):5367-79
pubmed: 12101232
Mol Cell Biol. 2005 Sep;25(18):8215-27
pubmed: 16135810
BMC Bioinformatics. 2013 Apr 15;14:128
pubmed: 23586463
Nucleic Acids Res. 2013 Jul;41(Web Server issue):W77-83
pubmed: 23703215
Cell Cycle. 2003 Jul-Aug;2(4):333-8
pubmed: 12851485
Nature. 1996 Jul 25;382(6589):319-24
pubmed: 8684459
Biochim Biophys Acta Gene Regul Mech. 2021 Feb;1864(2):194609
pubmed: 32730897
Genes Dev. 1997 Jul 1;11(13):1640-50
pubmed: 9224714
Mol Cell. 2017 Dec 7;68(5):955-969.e10
pubmed: 29220657
BMC Evol Biol. 2005 Feb 13;5:15
pubmed: 15707503
Cell Stem Cell. 2012 Aug 3;11(2):163-78
pubmed: 22862943
Genome Biol. 2014;15(12):550
pubmed: 25516281
Mol Cell. 2017 Oct 5;68(1):130-143.e5
pubmed: 28918903
Genes Dev. 2020 Oct 1;34(19-20):1287-1303
pubmed: 33004486
Nature. 2020 Jan;577(7792):717-720
pubmed: 31969703
Nature. 1981 Jul 9;292(5819):154-6
pubmed: 7242681
Cell Mol Life Sci. 2010 Feb;67(4):611-28
pubmed: 19936620
EMBO J. 2011 Jun 03;30(14):2843-52
pubmed: 21642955
Nat Commun. 2017 Oct 20;8(1):1088
pubmed: 29057918
Mol Cell Biol. 2010 Sep;30(17):4254-66
pubmed: 20584983
Mol Cell Biol. 2018 Nov 28;38(24):
pubmed: 30275344
J Biol Chem. 2002 Mar 8;277(10):7989-95
pubmed: 11773077
Dev Dyn. 2007 Jun;236(6):1547-57
pubmed: 17440986
EMBO J. 2010 Jul 21;29(14):2381-94
pubmed: 20562830
Front Cell Dev Biol. 2017 Feb 03;5:7
pubmed: 28217689
BMC Genomics. 2018 Mar 1;19(1):169
pubmed: 29490630
Nat Methods. 2012 Jun 28;9(7):676-82
pubmed: 22743772
Elife. 2017 Mar 13;6:
pubmed: 28287392
Nat Rev Mol Cell Biol. 2015 Mar;16(3):129-43
pubmed: 25693126
Mol Cell Biol. 2007 May;27(9):3405-16
pubmed: 17325035
Nat Commun. 2016 Oct 31;7:13227
pubmed: 27796307
Behav Brain Res. 2001 Nov 1;125(1-2):279-84
pubmed: 11682119
Curr Protoc Mol Biol. 2015 Jan 05;109:21.29.1-21.29.9
pubmed: 25559105
Curr Protoc. 2021 Mar;1(3):e90
pubmed: 33780170
Mol Cell Biol. 2009 Mar;29(5):1176-88
pubmed: 19103755
G3 (Bethesda). 2018 Jan 4;8(1):79-89
pubmed: 29118030
Genes Dev. 2012 Mar 15;26(6):527-41
pubmed: 22426530
Cold Spring Harb Perspect Med. 2013 Dec 01;3(12):a014381
pubmed: 24296349
EMBO J. 1995 Apr 3;14(7):1520-31
pubmed: 7729427
Mol Cell. 2011 Nov 4;44(3):410-423
pubmed: 22055187
Nat Genet. 2017 Nov;49(11):1647-1653
pubmed: 28967912
Genome Biol. 2010;11(10):R106
pubmed: 20979621
Nat Rev Cancer. 2009 Nov;9(11):785-97
pubmed: 19851314
Mol Cell Biol. 2009 Jan;29(1):266-80
pubmed: 18936164
J Biol Chem. 2012 Aug 24;287(35):29442-56
pubmed: 22736770
J Vis Exp. 2013 Aug 08;(78):
pubmed: 23963265
Nat Methods. 2013 Dec;10(12):1213-8
pubmed: 24097267
Differentiation. 2010 Jul;80(1):9-19
pubmed: 20537458
Genome Biol. 2008;9(9):R137
pubmed: 18798982
Genes Dev. 2003 Nov 1;17(21):2648-63
pubmed: 14563679
Nat Methods. 2012 Mar 04;9(4):357-9
pubmed: 22388286
Nucleic Acids Res. 2019 Jan 8;47(D1):D442-D450
pubmed: 30395289
Stem Cell Reports. 2018 Jan 9;10(1):287-299
pubmed: 29249668
Nucleic Acids Res. 2001 May 1;29(9):e45
pubmed: 11328886
Mol Cell. 2010 May 28;38(4):576-89
pubmed: 20513432
Science. 2016 Jun 3;352(6290):1225-8
pubmed: 27257258
Cell Rep. 2017 Apr 25;19(4):671-679
pubmed: 28445719
Proc Natl Acad Sci U S A. 1981 Dec;78(12):7634-8
pubmed: 6950406
PLoS One. 2009;4(5):e5553
pubmed: 19436741
Trends Biochem Sci. 2017 Nov;42(11):850-861
pubmed: 28964624
Nucleic Acids Res. 2016 Jul 8;44(W1):W90-7
pubmed: 27141961
Cell Rep. 2016 Apr 5;15(1):210-218
pubmed: 27052166
Development. 2017 Oct 15;144(20):3808-3818
pubmed: 28893950
Nature. 2020 Jan;577(7792):711-716
pubmed: 31969704
Nat Genet. 2000 Oct;26(2):229-32
pubmed: 11017084
Mol Cell. 2008 Jan 18;29(1):92-101
pubmed: 18206972
EMBO J. 2014 Nov 3;33(21):2534-46
pubmed: 25216679
Cell. 2011 Mar 18;144(6):940-54
pubmed: 21414485
Biochim Biophys Acta Gene Regul Mech. 2021 Feb;1864(2):194614
pubmed: 32739556
Mol Cell Biol. 1999 Jan;19(1):86-98
pubmed: 9858534
J Biol Chem. 2013 Aug 16;288(33):24234-46
pubmed: 23760504
Nat Commun. 2018 Sep 14;9(1):3759
pubmed: 30217978
Mol Syst Biol. 2011 Jul 05;7:503
pubmed: 21734642
EMBO J. 2001 Jun 15;20(12):3187-96
pubmed: 11406595
Nat Biotechnol. 2011 May;29(5):436-42
pubmed: 21516085
Stem Cell Reports. 2018 Jul 10;11(1):1-3
pubmed: 29996086
Bioinformatics. 2010 Mar 15;26(6):841-2
pubmed: 20110278
Development. 2019 Feb 22;146(4):
pubmed: 30718289
Mol Cell. 2019 Jun 20;74(6):1148-1163.e7
pubmed: 31005419
Cell Death Differ. 2021 Aug;28(8):2385-2403
pubmed: 33731875
Nat Commun. 2019 Jul 3;10(1):2939
pubmed: 31270324
EMBO J. 1993 Feb;12(2):489-99
pubmed: 8440239
Nat Commun. 2019 Nov 20;10(1):5237
pubmed: 31748520

Auteurs

Veronique Fischer (V)

Institut de Génétique et de Biologie Moléculaire et Cellulaire, 67404 Illkirch Cedex, France; Centre National de la Recherche Scientifique, UMR7104, 67404 Illkirch Cedex, France; Institut National de la Santé et de la Recherche Médicale, U964, 67404 Illkirch Cedex, France; Université de Strasbourg, 67000 Strasbourg, France.

Damien Plassard (D)

Institut de Génétique et de Biologie Moléculaire et Cellulaire, 67404 Illkirch Cedex, France; Centre National de la Recherche Scientifique, UMR7104, 67404 Illkirch Cedex, France; Institut National de la Santé et de la Recherche Médicale, U964, 67404 Illkirch Cedex, France; Université de Strasbourg, 67000 Strasbourg, France; Plateforme GenomEast, infrastructure France Génomique, Illkirch, France.

Tao Ye (T)

Institut de Génétique et de Biologie Moléculaire et Cellulaire, 67404 Illkirch Cedex, France; Centre National de la Recherche Scientifique, UMR7104, 67404 Illkirch Cedex, France; Institut National de la Santé et de la Recherche Médicale, U964, 67404 Illkirch Cedex, France; Université de Strasbourg, 67000 Strasbourg, France; Plateforme GenomEast, infrastructure France Génomique, Illkirch, France.

Bernardo Reina-San-Martin (B)

Institut de Génétique et de Biologie Moléculaire et Cellulaire, 67404 Illkirch Cedex, France; Centre National de la Recherche Scientifique, UMR7104, 67404 Illkirch Cedex, France; Institut National de la Santé et de la Recherche Médicale, U964, 67404 Illkirch Cedex, France; Université de Strasbourg, 67000 Strasbourg, France.

Matthieu Stierle (M)

Institut de Génétique et de Biologie Moléculaire et Cellulaire, 67404 Illkirch Cedex, France; Centre National de la Recherche Scientifique, UMR7104, 67404 Illkirch Cedex, France; Institut National de la Santé et de la Recherche Médicale, U964, 67404 Illkirch Cedex, France; Université de Strasbourg, 67000 Strasbourg, France.

Laszlo Tora (L)

Institut de Génétique et de Biologie Moléculaire et Cellulaire, 67404 Illkirch Cedex, France; Centre National de la Recherche Scientifique, UMR7104, 67404 Illkirch Cedex, France; Institut National de la Santé et de la Recherche Médicale, U964, 67404 Illkirch Cedex, France; Université de Strasbourg, 67000 Strasbourg, France.

Didier Devys (D)

Institut de Génétique et de Biologie Moléculaire et Cellulaire, 67404 Illkirch Cedex, France; Centre National de la Recherche Scientifique, UMR7104, 67404 Illkirch Cedex, France; Institut National de la Santé et de la Recherche Médicale, U964, 67404 Illkirch Cedex, France; Université de Strasbourg, 67000 Strasbourg, France. Electronic address: devys@igbmc.fr.

Articles similaires

Evaluating the efficacy of telesurgery with dual console SSI Mantra Surgical Robotic System: experiment on animal model and clinical trials.

Sudhir Prem Srivastava, Vishwajyoti Pascual Srivastava, Avinesh Singh et al.
1.00
Robotic Surgical Procedures Animals Humans Telemedicine Models, Animal

Odour generalisation and detection dog training.

Lyn Caldicott, Thomas W Pike, Helen E Zulch et al.
1.00
Animals Odorants Dogs Generalization, Psychological Smell

FBXO22 inhibits colitis and colorectal carcinogenesis by regulating the degradation of the S2448-phosphorylated form of mTOR.

Minle Li, Xuan Chen, Pengfei Qu et al.
1.00
Animals TOR Serine-Threonine Kinases Colorectal Neoplasms Colitis Mice

Use of organic material provided by an automatic enrichment device by weaner pigs and its influence on tail lesions.

Philipp Heseker, Jeanette Probst, Stefanie Ammer et al.
1.00
Animals Tail Swine Behavior, Animal Animal Husbandry

Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › The related coactivator complexes SAGA and...
questionsmedicales.fr › Phénomènes physiologiques › Croissance et développement › Croissance › Processus de croissance cellulaire › Prolifération cellulaire › Division cellulaire › Auto-renouvellement cellulaire › The related coactivator complexes SAGA and...
questionsmedicales.fr › Cellules › Cellules souches › Cellules souches pluripotentes › Cellules souches embryonnaires › The related coactivator complexes SAGA and...
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Transferases › Acyltransferases › Acetyltransferases › Lysine acetyltransferases › Histone acetyltransferases › The related coactivator complexes SAGA and...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Nucléoprotéines › Histone › The related coactivator complexes SAGA and...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Souris › The related coactivator complexes SAGA and...
questionsmedicales.fr › Phénomènes génétiques › Régulation de l'expression des gènes › Modification traductionnelle des protéines › Maturation post-traductionnelle des protéines › The related coactivator complexes SAGA and...
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Transferases › Phosphotransferases › Nucleotidyltransferases › RNA nucleotidyltransferases › DNA-directed RNA polymerases › RNA polymerase II › The related coactivator complexes SAGA and...
questionsmedicales.fr › Eucaryotes › Champignons › Levures › Saccharomyces › Saccharomyces cerevisiae › The related coactivator complexes SAGA and...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines fongiques › Protéines de Saccharomyces cerevisiae › The related coactivator complexes SAGA and...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines virales › Protéines virales régulatrices ou accessoires › Transactivateurs › The related coactivator complexes SAGA and...