Antibiotic resistance and random amplified polymorphic DNA typing of Klebsiella pneumoniae isolated from clinical and water samples.
Klebsiella pneumoniae
PCR
RAPD
antibiotics
environmental health
microbial contamination
wastewater
Journal
Water environment research : a research publication of the Water Environment Federation
ISSN: 1554-7531
Titre abrégé: Water Environ Res
Pays: United States
ID NLM: 9886167
Informations de publication
Date de publication:
Nov 2021
Nov 2021
Historique:
revised:
15
08
2021
received:
27
04
2021
accepted:
17
08
2021
pubmed:
26
8
2021
medline:
4
11
2021
entrez:
25
8
2021
Statut:
ppublish
Résumé
The study aimed to screen for the presence of multidrug-resistant Klebsiella pneumoniae from diarrheal stool and environmental water samples and to check the epidemiological link between the two categories. Isolates obtained after culturing on different media were tested for antibiotic resistance and extended-spectrum beta-lactamase (ESBL) production. Polymerase chain reaction (PCR) analysis was done for important β-lactamase encoding genes. Random amplified polymorphic DNA (RAPD) typing was done using two primers. Results showed a high prevalence of K. pneumoniae from fish market effluents compared with stool and well water. Stool isolates showed high resistance to ceftazidime (80.0%) and cefepime (80.0%), fish market effluent isolates to cefoperazone-sulbactam (92.1%), and erythromycin (78.9%), while well water isolates to erythromycin (72.7%) and cefuroxime (54.4%). The ESBL genes bla
Substances chimiques
Anti-Bacterial Agents
0
Water
059QF0KO0R
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
2740-2753Subventions
Organisme : NITTE (Deemed to be University)
ID : NUFR3/2017/06/03
Informations de copyright
© 2021 Water Environment Federation.
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