Proteomic profiling dataset of chemical perturbations in multiple biological backgrounds.
Journal
Scientific data
ISSN: 2052-4463
Titre abrégé: Sci Data
Pays: England
ID NLM: 101640192
Informations de publication
Date de publication:
25 08 2021
25 08 2021
Historique:
received:
08
01
2021
accepted:
07
05
2021
entrez:
26
8
2021
pubmed:
27
8
2021
medline:
1
10
2021
Statut:
epublish
Résumé
While gene expression profiling has traditionally been the method of choice for large-scale perturbational profiling studies, proteomics has emerged as an effective tool in this context for directly monitoring cellular responses to perturbations. We previously reported a pilot library containing 3400 profiles of multiple perturbations across diverse cellular backgrounds in the reduced-representation phosphoproteome (P100) and chromatin space (Global Chromatin Profiling, GCP). Here, we expand our original dataset to include profiles from a new set of cardiotoxic compounds and from astrocytes, an additional neural cell model, totaling 5300 proteomic signatures. We describe filtering criteria and quality control metrics used to assess and validate the technical quality and reproducibility of our data. To demonstrate the power of the library, we present two case studies where data is queried using the concept of "connectivity" to obtain biological insight. All data presented in this study have been deposited to the ProteomeXchange Consortium with identifiers PXD017458 (P100) and PXD017459 (GCP) and can be queried at https://clue.io/proteomics .
Identifiants
pubmed: 34433823
doi: 10.1038/s41597-021-01008-4
pii: 10.1038/s41597-021-01008-4
pmc: PMC8387426
doi:
Substances chimiques
Antineoplastic Agents
0
Cardiotoxins
0
Protein Kinase Inhibitors
0
Proteome
0
Types de publication
Dataset
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
226Subventions
Organisme : NCI NIH HHS
ID : U24 CA210986
Pays : United States
Organisme : U.S. Department of Health & Human Services | NIH | National Human Genome Research Institute (NHGRI)
ID : U54-HG008097
Organisme : NHGRI NIH HHS
ID : U54 HG008097
Pays : United States
Organisme : NCI NIH HHS
ID : U01 CA214125
Pays : United States
Organisme : U.S. Department of Health & Human Services | NIH | National Cancer Institute (NCI)
ID : U01-CA214125
Organisme : U.S. Department of Health & Human Services | NIH | National Cancer Institute (NCI)
ID : U24-CA210986
Informations de copyright
© 2021. The Author(s).
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