Optimization of Crude Oil Biodegradation by
Associated bacteria
Dictyonella Sp
Gas Chromatography
Taguchi method
Journal
Iranian journal of biotechnology
ISSN: 1728-3043
Titre abrégé: Iran J Biotechnol
Pays: Iran
ID NLM: 101276796
Informations de publication
Date de publication:
Apr 2021
Apr 2021
Historique:
entrez:
26
8
2021
pubmed:
27
8
2021
medline:
27
8
2021
Statut:
epublish
Résumé
The native sponges of Persian Gulf are unique species facing difficult climate conditions and environmental contamination. It is necessary to investigate these native sponges because global warming most probably destroyed many of these creatures. Therefore, the study of the microorganisms associated with sponges will introduce new bacterial strains with various industrial and environmental applications and, in this way, a part of the Persian Gulf biodiversity will be preserved for posterity. The aim of this study was the isolation and molecular identification of bacteria associated with the ability of biodegrading crude oil from the native sponges of the Persian Gulf. Also, optimization of crude oil biodegradation was done for one of the most efficient bacterial strains. Isolated species were compared in terms of E24 index and growth rate in a culture medium containing at least 2% of oil as the sole carbon source. Molecular identification was done for five bacterial strains. Using the Taguchi experimental design, the effects of 4 factors, namely, carbon source auxiliary, organic and inorganic nitrogen sources, salinity and pH, were evaluated at 3 levels. GC-Mass analysis was performed on the remaining oil in the culture medium. In the initial screening of two native species of sponges, 22 bacterial strains were isolated which were capable of decomposing oil. Five bacterial strains showed the best results and were recorded in NCBI with access numbers KY283126, KY283128, KY285290, KY285289, and KY285288. This bacterial strain can be used for biodegradation in oil-contaminated areas and oil refineries. By isolating the oil degrading gene in this bacterial strain and cloning it in other bacterial strains, the efficiency of eliminating oil contamination can be increased.
Sections du résumé
BACKGROUND
BACKGROUND
The native sponges of Persian Gulf are unique species facing difficult climate conditions and environmental contamination. It is necessary to investigate these native sponges because global warming most probably destroyed many of these creatures. Therefore, the study of the microorganisms associated with sponges will introduce new bacterial strains with various industrial and environmental applications and, in this way, a part of the Persian Gulf biodiversity will be preserved for posterity.
OBJECTIVE
OBJECTIVE
The aim of this study was the isolation and molecular identification of bacteria associated with the ability of biodegrading crude oil from the native sponges of the Persian Gulf. Also, optimization of crude oil biodegradation was done for one of the most efficient bacterial strains.
MATERIALS AND METHODS
METHODS
Isolated species were compared in terms of E24 index and growth rate in a culture medium containing at least 2% of oil as the sole carbon source. Molecular identification was done for five bacterial strains. Using the Taguchi experimental design, the effects of 4 factors, namely, carbon source auxiliary, organic and inorganic nitrogen sources, salinity and pH, were evaluated at 3 levels. GC-Mass analysis was performed on the remaining oil in the culture medium.
RESULTS
RESULTS
In the initial screening of two native species of sponges, 22 bacterial strains were isolated which were capable of decomposing oil. Five bacterial strains showed the best results and were recorded in NCBI with access numbers KY283126, KY283128, KY285290, KY285289, and KY285288.
CONCLUSIONS
CONCLUSIONS
This bacterial strain can be used for biodegradation in oil-contaminated areas and oil refineries. By isolating the oil degrading gene in this bacterial strain and cloning it in other bacterial strains, the efficiency of eliminating oil contamination can be increased.
Identifiants
pubmed: 34435057
doi: 10.30498/IJB.2021.2690
pii: IJB-19-2
pmc: PMC8358178
doi:
Types de publication
Journal Article
Langues
eng
Pagination
e2690Informations de copyright
Copyright: © 2021 The Author(s); Published by Iranian Journal of Biotechnology.
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